This long-pass set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from DAPI and other similar fluorophores while offering superb blocking of popular 405 nm or 375 nm solid-state laser diodes. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.
Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF405/LP-C
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,315.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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0.000.00 -
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- Notify me when available
We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
Set Components
BLP01-405R-25FL-008529
Each
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FL-008529 |
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Di02-R405-25x36FL-007134
Each
Custom sizing delivery confirmed ARO (sizing fee applies). |
FL-007134 |
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FF01-390/40-25FL-004666
Each
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FL-004666 |
total selected
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LF405/LP-C-000
S-000885 / 0
1λ P-V RWE super-resolution laser optimized long-pass filter set. Ideal for use with: DAPI, Alexa Fluor® 405, ATTO 390, BFP, BV421, Cascade Blue™, DyLight405
LF405/LP-B-NQF
S-001768 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
LF405/LP-B-NTE
S-000446 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
LF405/LP-B-OMF
S-000447 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
LF405/LP-B-ZHE
S-001770 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
LF405/LP-B-OFF
S-001769 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63
1 of 1
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
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| 4-methylumbelliferone | 357 | 441 |
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| 7-hydroxy-4-methylcoumarin | 360 | 442 |
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| Alexa Fluor® 350 | 343 | 441 |
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| Alexa Fluor® 405 | 401 | 422 |
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| Alexa Fluor® 430 | 431 | 541 |
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| AMCA (Aminomethylcoumarin) | 350 | 448 |
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| Aqua 431 | 430 | 478 |
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| ATTO 390 | 392 | 477 |
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| ATTO 425 | 436 | 484 |
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| BD Horizon V450 | 406 | 449 |
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| BD Horizon V500 | 410 | 500 |
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| BFP (EBFP) | 380 | 440 |
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| Calcofluor White | 351 | 444 |
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| Cascade Blue™ | 401 | 419 |
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| Cascade Yellow™ | 399 | 549 |
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| CellTrace calcein violet | 400 | 452 |
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| CellTracker Violet BMQC+GSH | 406 | 526 |
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| CFP (ECFP) | 433 | 475 |
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| ChlorophyllA | 480 | 650 |
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| DAPI | 359 | 461 |
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| Dapoxyl | 373 | 579 |
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| DEAC | 432 | 472 |
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| DiFMU-pH 9.0 | 358 | 450 |
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| DY-415 | 418 | 470 |
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| DyLight 405 | 398 | 420 |
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| ER-Tracker™ Blue-White DPX | 372 | 557 |
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| Fura Red™ (high pH) | 572 | 657 |
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| Fura-2 (calcium free) | 366 | 515 |
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| GFPuv | 395 | 509 |
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| HiLyte Fluor™ 405 | 403 | 428 |
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| Hoechst 33258 | 352 | 455 |
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| Hoechst 33342 | 352 | 455 |
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| Hoechst 34580 | 392 | 440 |
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| LIVE-DEAD Fixable Aqua Dead Cell Stain | 367 | 526 |
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| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
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| Lucifer yellow | 428 | 544 |
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| LysoSensor Blue | 374 | 424 |
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| LysoTracker Blue | 373 | 422 |
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| Marina Blue | 363 | 461 |
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| mBBr+GSH | 394 | 490 |
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| Pacific Blue™ | 404 | 455 |
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| Pacific Orange | 400 | 551 |
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| Qdot® 525 Nanocrystals | 300 | 525 |
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| Qdot® 545 Nanocrystals | 300 | 543 |
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| Qdot® 565 Nanocrystals | 300 | 564 |
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| Qdot® 585 Nanocrystals | 300 | 588 |
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| Qdot® 605 Nanocrystals | 300 | 602 |
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| Qdot® 625 Nanocrystals | 300 | 621 |
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| Qdot® 655 Nanocrystals | 300 | 654 |
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| Qdot® 705 Nanocrystals | 300 | 702 |
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| Qdot® 800 Nanocrystals | 300 | 792 |
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| sgBFP™ | 387 | 451 |
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| Sirius | 355 | 424 |
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| SpectrumAqua | 434 | 481 |
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| SpectrumBlue | 405 | 449 |
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| SYPRO Ruby protein gel stain | 469 | 631 |
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| TagBFP | 402 | 457 |
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| ThiolTracker Violet+GSH | 404 | 526 |
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| Vybrant DyeCycle Violet | 369 | 437 |
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