BrightLine® single-band 1λ P-V RWE super-resolution laser filter set, optimized for 405 nm laser sources

Semrock Part Number: LF405-C-OMF

IDEX Part Number: S-000454  /

Semrock

This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from DAPI and other similar fluorophores while offering superb blocking of popular 405 nm or 375 nm solid-state laser diodes. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.

Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.

Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?
Our previous version is still available for those cube configurations LF405-B

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,842.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • 0.00
    0.00
  • Notify me when available

FF01-452/45-25

FL-004300 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 429.5 – 474.5 nm
  • Center Wavelength of 452 nm and GMBW of 45 nm
  • FWHM of 50.5 nm

FF01-390/40-25

FL-004666 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 370 – 410 nm
  • Center Wavelength of 390 nm and GMBW of 40 nm
  • FWHM of 44.6 nm

Di03-R405-t1-25x36

FL-007078 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 414 nm,
  • Rabs > 94% 372 – 410 nm
  • Ravg > 90% 350 – 372 nm
  • Tavg > 93% 417.4 – 1200 nm
  •  Flatness / RWE: Super-resolution / TIRF

1 of 1

This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
4-methylumbelliferone 357 441
7-hydroxy-4-methylcoumarin 360 442
Alexa Fluor® 350 343 441
Alexa Fluor® 405 401 422
AMCA (Aminomethylcoumarin) 350 448
ATTO 390 392 477
BD Horizon V450 406 449
BFP (EBFP) 380 440
BV421 - BD Horizon Brilliant™ Violet 421 407 421
Calcofluor White 351 444
Cascade Blue™ 401 419
CellTrace calcein violet 400 452
CFP (ECFP) 433 475
DAPI 359 461
DEAC 432 472
DiFMU-pH 9.0 358 450
DY-415 418 470
DyLight 350 351 434
DyLight 405 398 420
ER-Tracker™ Blue-White DPX 372 557
HiLyte Fluor™ 405 403 428
Hoechst 33258 352 455
Hoechst 33342 352 455
Hoechst 34580 392 440
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
LysoSensor Blue 374 424
LysoTracker Blue 373 422
Marina Blue 363 461
mBBr+GSH 394 490
Pacific Blue™ 404 455
sgBFP™ 387 451
Sirius 355 424
SpectrumBlue 405 449
TagBFP 402 457
Vybrant DyeCycle Violet 369 437

Related Products

FF01-452/45-25

FL-004300 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 429.5 – 474.5 nm
  • Center Wavelength of 452 nm and GMBW of 45 nm
  • FWHM of 50.5 nm

FF01-390/40-25

FL-004666 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 370 – 410 nm
  • Center Wavelength of 390 nm and GMBW of 40 nm
  • FWHM of 44.6 nm

Di03-R405-t1-25x36

FL-007078 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 414 nm,
  • Rabs > 94% 372 – 410 nm
  • Ravg > 90% 350 – 372 nm
  • Tavg > 93% 417.4 – 1200 nm
  •  Flatness / RWE: Super-resolution / TIRF

1 of 1

This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.