This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from DAPI and other similar fluorophores while offering superb blocking of popular 405 nm or 375 nm solid-state laser diodes. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.
Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF405-C
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,744.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-452/45-25
FL-004300 / 0
- Tavg > 93% 429.5 – 474.5 nm
- Center Wavelength of 452 nm and GMBW of 45 nm
- FWHM of 50.5 nm
FF01-390/40-25
FL-004666 / 0
- Tavg > 93% 370 – 410 nm
- Center Wavelength of 390 nm and GMBW of 40 nm
- FWHM of 44.6 nm
Di02-R405-25x36
FL-007134 / 0
- Laser Dichroic Beamsplitter with edge at 414 nm
- Rabs > 94% 372 – 410 nm
- Ravg > 90% 350 – 372 nm
- Tavg > 93% 417.4 – 900 nm
- Flatness / RWE: Laser
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in a Leica Microsystems DMi8 (“P-Cube”) Fluorescence Filter Holder, which is compatible with the DMi8 Leica microscope.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
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| 4-methylumbelliferone | 357 | 441 |
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| 7-hydroxy-4-methylcoumarin | 360 | 442 |
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| Alexa Fluor® 350 | 343 | 441 |
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| Alexa Fluor® 405 | 401 | 422 |
|
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| AMCA (Aminomethylcoumarin) | 350 | 448 |
|
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| ATTO 390 | 392 | 477 |
|
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| BD Horizon V450 | 406 | 449 |
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| BFP (EBFP) | 380 | 440 |
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| Calcofluor White | 351 | 444 |
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| Cascade Blue™ | 401 | 419 |
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| CellTrace calcein violet | 400 | 452 |
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| CFP (ECFP) | 433 | 475 |
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| DAPI | 359 | 461 |
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| DEAC | 432 | 472 |
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| DiFMU-pH 9.0 | 358 | 450 |
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| DY-415 | 418 | 470 |
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| DyLight 405 | 398 | 420 |
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| ER-Tracker™ Blue-White DPX | 372 | 557 |
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| HiLyte Fluor™ 405 | 403 | 428 |
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| Hoechst 33258 | 352 | 455 |
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| Hoechst 33342 | 352 | 455 |
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| Hoechst 34580 | 392 | 440 |
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| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
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| LysoSensor Blue | 374 | 424 |
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| LysoTracker Blue | 373 | 422 |
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| Marina Blue | 363 | 461 |
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| mBBr+GSH | 394 | 490 |
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| Pacific Blue™ | 404 | 455 |
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| sgBFP™ | 387 | 451 |
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| Sirius | 355 | 424 |
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| SpectrumBlue | 405 | 449 |
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| TagBFP | 402 | 457 |
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| Vybrant DyeCycle Violet | 369 | 437 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-452/45-25
FL-004300 / 0
- Tavg > 93% 429.5 – 474.5 nm
- Center Wavelength of 452 nm and GMBW of 45 nm
- FWHM of 50.5 nm
FF01-390/40-25
FL-004666 / 0
- Tavg > 93% 370 – 410 nm
- Center Wavelength of 390 nm and GMBW of 40 nm
- FWHM of 44.6 nm
Di02-R405-25x36
FL-007134 / 0
- Laser Dichroic Beamsplitter with edge at 414 nm
- Rabs > 94% 372 – 410 nm
- Ravg > 90% 350 – 372 nm
- Tavg > 93% 417.4 – 900 nm
- Flatness / RWE: Laser
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in a Leica Microsystems DMi8 (“P-Cube”) Fluorescence Filter Holder, which is compatible with the DMi8 Leica microscope.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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