This full-multiband set is optimized for laser excitation utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, and a 593.5, 594.0 or 594.1 nm laser source for mCherry. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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2,099.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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| Laser Wavelength 1 (high) | 491 nm, 594.3 nm |
|---|---|
| Laser Wavelength 1 (low) | 473 nm, 593.5 nm |
| Laser Wavelength 2 | 488 nm |
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FF01-390/482/587-25
FL-004251 / 0
- Tavg > 85% 370 – 410 nm, Tavg > 93% 473 – 491 nm & Tavg > 93% 579.3 – 594.3 nm
- Center Wavelengths of 390 nm, 482 nm & 587 nm and GMBWs of 40 nm, 18 nm & 15 nm
FF01-446/532/646-25
FL-004254 / 0
- Tavg > 93% 429.5 – 462 nm, Tavg > 93% 502.5 – 561.0 nm & Tavg > 93% 612 – 680 nm
- Center Wavelengths of 446 nm, 532 nm & 646 nm and GMBWs of 36 nm, 23 nm & 61 nm
Di01-R405/488/594-25x36
FL-006958 / 0
- Laser Dichroic Beamsplitter with edges at 420 nm, 497 nm & 603 nm
- Rabs > 94% 370 – 410 nm, Rabs > 94% 473 – 491 nm & Ravg > 94% 588.3 – 594.3 nm
- Tavg > 93% 429.5 – 462.0 nm, Tavg > 93% 502.5 – 574.5 nm & Tavg > 93% 612.0 – 800.0 nm
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This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
|
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| 2-dodecylresorufin-lipid | 582 | 595 |
|
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| 4-methylumbelliferone | 357 | 441 |
|
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
|
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| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
|
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
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| 6-carboxyrhodamine 6G | 525 | 555 |
|
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
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| 7-hydroxy-4-methylcoumarin | 360 | 442 |
|
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| Acridine Orange (+DNA) | 502 | 526 |
|
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| Alexa Fluor® 405 | 401 | 422 |
|
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| Alexa Fluor® 430 | 431 | 541 |
|
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| Alexa Fluor® 488 | 499 | 520 |
|
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| Alexa Fluor® 500 | 503 | 525 |
|
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| Alexa Fluor® 568 | 579 | 603 |
|
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| Alexa Fluor® 594 | 590 | 618 |
|
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| AMCA (Aminomethylcoumarin) | 350 | 448 |
|
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| AmCyan1 | 458 | 489 |
|
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| APC | 651 | 660 |
|
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| APC (Allophycocyanin) | 651 | 660 |
|
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| AsRed 2 | 576 | 592 |
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| ATTO 390 | 392 | 477 |
|
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| ATTO 488 | 500 | 525 |
|
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| ATTO 495 | 495 | 527 |
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| ATTO 590 | 594 | 624 |
|
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| ATTO 594 | 601 | 624 |
|
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| ATTO 610 | 614 | 634 |
|
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| BCECF (pH 5.5) | 481 | 518 |
|
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| BCECF (pH 9.0) | 502 | 528 |
|
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| BD Horizon V450 | 406 | 449 |
|
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| BFP (EBFP) | 380 | 440 |
|
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| BOBO™-3 | 570 | 605 |
|
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| BODIPY 630/650-X | 625 | 641 |
|
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| BODIPY FL | 505 | 512 |
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| BODIPY FL-MeOH | 502 | 511 |
|
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| BODIPY TR-X phallacidin | 590 | 621 |
|
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| Calcein | 494 | 514 |
|
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| Calcium Crimson | 589 | 609 |
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| Calcium Green-1 | 507 | 529 |
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| Calcofluor White | 351 | 444 |
|
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| Carboxynaphthofluorescein | 599 | 674 |
|
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| Cascade Blue™ | 401 | 419 |
|
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| CellTrace BODIPY TR methyl ester | 598 | 625 |
|
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| CellTrace calcein violet | 400 | 452 |
|
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| CellTracker Red CMTPX | 586 | 614 |
|
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| CFP2 | 490 | 510 |
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| ChlorophyllA | 480 | 650 |
|
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| Cy2™ | 492 | 507 |
|
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| Cy3.5™ | 578 | 591 |
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| CyQUANT GR-DNA | 502 | 523 |
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| DAF-FM-NO | 495 | 519 |
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| DAPI | 359 | 461 |
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| DDAO | 648 | 657 |
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| Dendra2 (Green) | 491 | 507 |
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| DiA | 457 | 586 |
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| DiFMU-pH 9.0 | 358 | 450 |
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| DiO | 475 | 500 |
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| Dronpa | 502 | 516 |
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| DY-415 | 418 | 470 |
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| DY-505-Phalloidin | 503 | 530 |
|
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| DY-590 | 582 | 599 |
|
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| DyLight 405 | 398 | 420 |
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| DyLight 594 | 593 | 618 |
|
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| DyLight 633 | 620 | 649 |
|
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| ecliptic pHluorin pH5.5 | 473 | 507 |
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| Emerald | 491 | 511 |
|
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| ER-Tracker™ Blue-White DPX | 372 | 557 |
|
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| evoglow-Bs1 | 449 | 496 |
|
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| FITC (Fluorescein) | 495 | 519 |
|
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| FlAsH-CCPFCC | 511 | 530 |
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| Fluo-3 | 506 | 527 |
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| Fluo-4 | 494 | 516 |
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| Fluorescein dextran | 501 | 524 |
|
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| Fluorescein-pH 8.0 | 489 | 517 |
|
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| Fluoro-Emerald | 494 | 518 |
|
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| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
|
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| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
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| FM 1-43 | 473 | 579 |
|
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| GFP (EGFP) | 489 | 511 |
|
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| Green 496 | 496 | 520 |
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| Green 500 | 501 | 524 |
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| HcRed1 | 588 | 618 |
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| HCS CellMask Red cytoplasmic | 622 | 644 |
|
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| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
|
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| HCS LipidTOX Green neutral lipid stain | 498 | 507 |
|
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
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| HiLyte Fluor™ 405 | 403 | 428 |
|
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| HiLyte Fluor™ 488 | 497 | 526 |
|
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| HiLyte Fluor™ 594 | 592 | 616 |
|
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| Hoechst 33258 | 352 | 455 |
|
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| Hypericin | 600 | 603 |
|
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| KFP-Red | 575 | 599 |
|
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| LDS 751 | 560 | 711 |
|
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
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| LIVE-DEAD Fixable Red Dead Cell Stain | 595 | 613 |
|
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| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
|
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| LysoSensor Blue | 374 | 424 |
|
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| LysoSensor Green | 448 | 502 |
|
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| LysoTracker Blue | 373 | 422 |
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| LysoTracker Green | 501 | 509 |
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| LysoTracker Yellow HCK-123 | 488 | 565 |
|
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| Magnesium Green | 507 | 531 |
|
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| mApple | 566 | 594 |
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| Marina Blue | 363 | 461 |
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| mCherry | 587 | 610 |
|
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| mHoneyDew | 478 | 562 |
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| MitoTracker™ Deep Red | 641 | 662 |
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| MitoTracker™ Green | 490 | 512 |
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| MitoTracker™ Red | 578 | 598 |
|
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| mKate2 | 588 | 633 |
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| mPlum | 589 | 649 |
|
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| mRFP1 | 584 | 700 |
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| mStrawberry | 574 | 596 |
|
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| mWasabi | 493 | 509 |
|
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| NBD-X (MeOH) | 467 | 538 |
|
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
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| Nile blue-EtOH | 631 | 660 |
|
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| Nile red-phospolipid | 553 | 637 |
|
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| Oregon Green™ 488 | 498 | 526 |
|
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| Oregon Green™ 514 | 513 | 532 |
|
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| Pacific Blue™ | 404 | 455 |
|
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| PicoGreen® | 502 | 522 |
|
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| PKH67 | 488 | 500 |
|
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| Qdot® 525 Nanocrystals | 300 | 525 |
|
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| Qdot® 545 Nanocrystals | 300 | 543 |
|
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| Qdot® 625 Nanocrystals | 300 | 621 |
|
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| Qdot® 655 Nanocrystals | 300 | 654 |
|
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| ratiometric pHluorin pH5 | 500 | 509 |
|
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| ReAsH-CCPGCC | 592 | 606 |
|
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| Red 580 | 580 | 603 |
|
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| Rhodamine 110 | 497 | 519 |
|
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| Rhodamine 123 | 507 | 529 |
|
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| Rhodamine Green | 497 | 523 |
|
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| Rhodol Green | 497 | 524 |
|
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| sgBFP™ | 387 | 451 |
|
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| sgGFP™ (super glow GFP) | 472 | 506 |
|
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| Sirius | 355 | 424 |
|
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| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
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| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
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| SNARF-1 514nm (ph 9.0) | 576 | 638 |
|
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| Sodium Green | 507 | 532 |
|
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| SpectrumBlue | 405 | 449 |
|
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| SpectrumGreen | 497 | 538 |
|
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| SpectrumRed | 587 | 615 |
|
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
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| SYTO 11 | 506 | 525 |
|
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| SYTO 13 | 448 | 506 |
|
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| SYTO 16 | 489 | 520 |
|
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| SYTO 17 | 619 | 638 |
|
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| SYTO 59 | 622 | 644 |
|
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| SYTO 61 | 618 | 650 |
|
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| SYTO 9 | 483 | 500 |
|
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
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| SYTOX Green-DNA | 504 | 524 |
|
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| TagBFP | 402 | 457 |
|
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| Texas Red dextran | 592 | 613 |
|
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| Texas Red DHPE | 584 | 608 |
|
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| Texas Red® | 592 | 614 |
|
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| TO-PRO-1 | 515 | 531 |
|
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| TOTO-1 | 514 | 531 |
|
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| TurboFP635(Katushka) | 591 | 638 |
|
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| TurboGFP | 482 | 503 |
|
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| Vybrant DyeCycle Green | 506 | 534 |
|
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| Vybrant DyeCycle Orange | 519 | 563 |
|
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| Vybrant DyeCycle Violet | 369 | 437 |
|
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| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
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| X-Rhod-1 Indicator | 580 | 601 |
|
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| YO-PRO-1 | 491 | 506 |
|
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| YO-PRO-3 | 613 | 629 |
|
|
| YOYO-1 | 491 | 508 |
|
|
| YOYO-3 | 611 | 631 |
|
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
total selected
Clear selection
Select all 3 products from all pages.
FF01-390/482/587-25
FL-004251 / 0
- Tavg > 85% 370 – 410 nm, Tavg > 93% 473 – 491 nm & Tavg > 93% 579.3 – 594.3 nm
- Center Wavelengths of 390 nm, 482 nm & 587 nm and GMBWs of 40 nm, 18 nm & 15 nm