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BrightLine® full-multiband laser filter set, optimized for 405, 488, & 594 nm laser sources

Semrock Part Number: LF405/488/594-A-NTE

IDEX Part Number: S-000443  /

Semrock
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
  • Price Not Available.
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    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 2,099.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Laser Wavelength 1 (high) 491 nm, 594.1 nm
Laser Wavelength 1 (low) 473 nm, 593.5 nm
Laser Wavelength 2 488 nm

This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept a 2mm dichroic)
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
2-dodecylresorufin-lipid 582 595
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-carboxynapthofluorescein (pH 10) 555 615
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
6-carboxyrhodamine 6G 525 555
7-AAD (7-aminoactinomycin D) 548 648
7-hydroxy-4-methylcoumarin 360 442
Acridine Orange (+DNA) 502 526
Alexa Fluor® 405 401 422
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
AMCA (Aminomethylcoumarin) 350 448
AmCyan1 458 489
APC 651 660
APC (Allophycocyanin) 651 660
AsRed 2 576 592
ATTO 390 392 477
ATTO 488 500 525
ATTO 495 495 527
ATTO 590 594 624
ATTO 594 601 624
ATTO 610 614 634
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BD Horizon V450 406 449
BFP (EBFP) 380 440
BOBO™-3 570 605
BODIPY 630/650-X 625 641
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
BODIPY TR-X phallacidin 590 621
Calcein 494 514
Calcium Crimson 589 609
Calcium Green-1 507 529
Calcofluor White 351 444
Carboxynaphthofluorescein 599 674
Cascade Blue™ 401 419
CellTrace BODIPY TR methyl ester 598 625
CellTrace calcein violet 400 452
CellTracker Red CMTPX 586 614
CFP2 490 510
ChlorophyllA 480 650
Cy2™ 492 507
Cy3.5™ 578 591
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
DAPI 359 461
DDAO 648 657
Dendra2 (Green) 491 507
DiA 457 586
DiFMU-pH 9.0 358 450
DiO 475 500
Dronpa 502 516
DY-415 418 470
DY-505-Phalloidin 503 530
DY-590 582 599
DyLight 405 398 420
DyLight 594 593 618
DyLight 633 620 649
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
ER-Tracker™ Blue-White DPX 372 557
evoglow-Bs1 449 496
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Crimson fluorescent microspheres 620 646
FluoSpheres Red fluorescent microspheres 577 607
FluoSpheres Yellow-Green fluorescent microspheres 503 514
FM 1-43 473 579
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HcRed1 588 618
HCS CellMask Red cytoplasmic 622 644
HCS LipidTOX Deep Red neutral lipid stain 635 652
HCS LipidTOX Green neutral lipid stain 498 507
HCS LipidTOX Green phospholipidosis 504 536
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 405 403 428
HiLyte Fluor™ 488 497 526
HiLyte Fluor™ 594 592 616
Hoechst 33258 352 455
Hypericin 600 603
KFP-Red 575 599
LDS 751 560 711
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LIVE-DEAD Fixable Red Dead Cell Stain 595 613
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
LysoSensor Blue 374 424
LysoSensor Green 448 502
LysoTracker Blue 373 422
LysoTracker Green 501 509
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
mApple 566 594
Marina Blue 363 461
mCherry 587 610
mHoneyDew 478 562
MitoTracker™ Deep Red 641 662
MitoTracker™ Green 490 512
MitoTracker™ Red 578 598
mKate2 588 633
mPlum 589 649
mRFP1 584 700
mStrawberry 574 596
mWasabi 493 509
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Nile blue-EtOH 631 660
Nile red-phospolipid 553 637
Oregon Green™ 488 498 526
Oregon Green™ 514 513 532
Pacific Blue™ 404 455
PicoGreen® 502 522
PKH67 488 500
Qdot® 525 Nanocrystals 300 525
Qdot® 545 Nanocrystals 300 543
Qdot® 625 Nanocrystals 300 621
Qdot® 655 Nanocrystals 300 654
ratiometric pHluorin pH5 500 509
ReAsH-CCPGCC 592 606
Red 580 580 603
Rhodamine 110 497 519
Rhodamine 123 507 529
Rhodamine Green 497 523
Rhodol Green 497 524
sgBFP™ 387 451
sgGFP™ (super glow GFP) 472 506
Sirius 355 424
SNARF (carboxy) 514 Excitation pH9 576 638
SNARF-1 488nm (ph 9.0) 576 638
SNARF-1 514nm (ph 9.0) 576 638
Sodium Green 507 532
SpectrumBlue 405 449
SpectrumGreen 497 538
SpectrumRed 587 615
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYTO 11 506 525
SYTO 13 448 506
SYTO 16 489 520
SYTO 17 619 638
SYTO 59 622 644
SYTO 61 618 650
SYTO 9 483 500
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
TagBFP 402 457
Texas Red dextran 592 613
Texas Red DHPE 584 608
Texas Red® 592 614
TO-PRO-1 515 531
TOTO-1 514 531
TurboFP635(Katushka) 591 638
TurboGFP 482 503
Vybrant DyeCycle Green 506 534
Vybrant DyeCycle Orange 519 563
Vybrant DyeCycle Violet 369 437
wtGFP (wild type GFP, non-UV excitation) 474 509
X-Rhod-1 Indicator 580 601
YO-PRO-1 491 506
YO-PRO-3 613 629
YOYO-1 491 508
YOYO-3 611 631

Specifications

This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept a 2mm dichroic)

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