This "Pinkel" multiband set is optimized for laser excitation utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, and a 593.5, 594.0 or 594.1 nm laser source for mCherry. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio.
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Laser Wavelength 1 (high) | 491 nm, 594.3 nm |
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Laser Wavelength 1 (low) | 473 nm, 593.5 nm |
Laser Wavelength 2 | 488 nm |
FL-004254 / 0
FL-004666 / 0
FL-004689 / 0
FL-004712 / 0
FL-006958 / 0
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This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 5.0 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
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1,8-ANS | 372 | 477 |
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4-methylumbelliferone | 357 | 441 |
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5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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5-FAM (5-carboxyfluorescein) | 492 | 518 |
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5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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6-carboxyrhodamine 6G | 525 | 555 |
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7-AAD (7-aminoactinomycin D) | 548 | 648 |
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7-hydroxy-4-methylcoumarin | 360 | 442 |
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Acridine Orange (+DNA) | 502 | 526 |
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Alexa Fluor® 405 | 401 | 422 |
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Alexa Fluor® 430 | 431 | 541 |
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Alexa Fluor® 488 | 499 | 520 |
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Alexa Fluor® 500 | 503 | 525 |
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Alexa Fluor® 568 | 579 | 603 |
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Alexa Fluor® 594 | 590 | 618 |
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AMCA (Aminomethylcoumarin) | 350 | 448 |
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AmCyan1 | 458 | 489 |
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APC | 651 | 660 |
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APC (Allophycocyanin) | 651 | 660 |
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AsRed 2 | 576 | 592 |
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ATTO 390 | 392 | 477 |
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ATTO 488 | 500 | 525 |
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ATTO 495 | 495 | 527 |
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ATTO 590 | 594 | 624 |
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ATTO 594 | 601 | 624 |
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ATTO 610 | 614 | 634 |
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ATTO 620 | 618 | 642 |
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ATTO 635 | 635 | 658 |
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ATTO 647 | 644 | 670 |
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BCECF (pH 5.5) | 481 | 518 |
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BCECF (pH 9.0) | 502 | 528 |
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BD Horizon V450 | 406 | 449 |
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BFP (EBFP) | 380 | 440 |
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BOBO™-3 | 570 | 605 |
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BODIPY 630/650-X | 625 | 641 |
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BODIPY FL | 505 | 512 |
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BODIPY FL-MeOH | 502 | 511 |
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BODIPY TR-X phallacidin | 590 | 621 |
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Calcein | 494 | 514 |
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Calcium Crimson | 589 | 609 |
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Calcium Green-1 | 507 | 529 |
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Calcofluor White | 351 | 444 |
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Carboxynaphthofluorescein | 599 | 674 |
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Cascade Blue™ | 401 | 419 |
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CellTrace BODIPY TR methyl ester | 598 | 625 |
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CellTrace calcein violet | 400 | 452 |
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CellTracker Red CMTPX | 586 | 614 |
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CFP2 | 490 | 510 |
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ChlorophyllA | 480 | 650 |
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Cy2™ | 492 | 507 |
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CyQUANT GR-DNA | 502 | 523 |
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DAF-FM-NO | 495 | 519 |
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DAPI | 359 | 461 |
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DDAO | 648 | 657 |
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Dendra2 (Green) | 491 | 507 |
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DiA | 457 | 586 |
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DiFMU-pH 9.0 | 358 | 450 |
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DiO | 475 | 500 |
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Dronpa | 502 | 516 |
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DY-415 | 418 | 470 |
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DY-505-Phalloidin | 503 | 530 |
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DyLight 405 | 398 | 420 |
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DyLight 594 | 593 | 618 |
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DyLight 633 | 620 | 649 |
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ecliptic pHluorin pH5.5 | 473 | 507 |
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Emerald | 491 | 511 |
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ER-Tracker™ Blue-White DPX | 372 | 557 |
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evoglow-Bs1 | 449 | 496 |
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FITC (Fluorescein) | 495 | 519 |
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FlAsH-CCPFCC | 511 | 530 |
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Fluo-3 | 506 | 527 |
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Fluo-4 | 494 | 516 |
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Fluorescein dextran | 501 | 524 |
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Fluorescein-pH 8.0 | 489 | 517 |
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Fluoro-Emerald | 494 | 518 |
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FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
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FluoSpheres Red fluorescent microspheres | 577 | 607 |
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FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
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FM 1-43 | 473 | 579 |
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GFP (EGFP) | 489 | 511 |
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Green 496 | 496 | 520 |
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Green 500 | 501 | 524 |
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HcRed1 | 588 | 618 |
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HCS CellMask Red cytoplasmic | 622 | 644 |
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HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
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HCS LipidTOX Green neutral lipid stain | 498 | 507 |
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HCS LipidTOX Green phospholipidosis | 504 | 536 |
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HCS LipidTOX Red neutral lipid stain | 582 | 616 |
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HCS LipidTOX Red phospholipidosis | 584 | 608 |
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HiLyte Fluor™ 405 | 403 | 428 |
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HiLyte Fluor™ 488 | 497 | 526 |
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HiLyte Fluor™ 594 | 592 | 616 |
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Hoechst 33258 | 352 | 455 |
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Hypericin | 600 | 603 |
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KFP-Red | 575 | 599 |
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LDS 751 | 560 | 711 |
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LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
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LIVE-DEAD Fixable Red Dead Cell Stain | 595 | 613 |
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LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
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LysoSensor Blue | 374 | 424 |
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LysoSensor Green | 448 | 502 |
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LysoTracker Blue | 373 | 422 |
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LysoTracker Green | 501 | 509 |
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LysoTracker Yellow HCK-123 | 488 | 565 |
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Magnesium Green | 507 | 531 |
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Marina Blue | 363 | 461 |
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mCherry | 587 | 610 |
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mHoneyDew | 478 | 562 |
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MitoTracker™ Deep Red | 641 | 662 |
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MitoTracker™ Green | 490 | 512 |
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mKate2 | 588 | 633 |
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mPlum | 589 | 649 |
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mRFP1 | 584 | 700 |
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mStrawberry | 574 | 596 |
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mWasabi | 493 | 509 |
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NBD-X (MeOH) | 467 | 538 |
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NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
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Nile blue-EtOH | 631 | 660 |
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Nile red-phospolipid | 553 | 637 |
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Oregon Green™ 488 | 498 | 526 |
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Oregon Green™ 514 | 513 | 532 |
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