BrightLine® Sedat laser filter set, optimized for 405, 488, & 594 nm laser sources

Semrock Part Number: LF405/488/594-3X3M-B-ZHE

IDEX Part Number: S-001761  /

Semrock

This "Sedat" multiband set is optimized for laser excitation utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, and a 593.5, 594.0 or 594.1 nm laser source for mCherry. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 0.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • 0.00
    0.00
  • Notify me when available
Laser Wavelength 1 (high) 491 nm, 594.3 nm
Laser Wavelength 1 (low) 473 nm, 593.5 nm
Laser Wavelength 2 488 nm

FF01-647/57-25

FL-004142 / 0

Out of Stock Backordered In Stock
  • Tavg > 92% 618.5 – 675.5 nm
  • Center Wavelength of 647 nm and GMBW of 57 nm
  • FWHM of 70 nm

FF01-445/20-25

FL-004531 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 435 – 455 nm
  • Center Wavelength of 445 nm and GMBW of 20 nm
  • FWHM of 25 nm

FF01-390/40-25

FL-004666 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 370 – 410 nm
  • Center Wavelength of 390 nm and GMBW of 40 nm
  • FWHM of 44.6 nm

FF01-525/45-25

FL-004680 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 502.5 – 547.5 nm
  • Center Wavelength of 525 nm and GMBW of 45 nm
  • FWHM of 50.6 nm

FF01-591/6-25

FL-004689 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 588 – 594.5 nm
  • Center Wavelength of 591 nm and GMBW of 6 nm
  • FWHM of 10.0 nm

FF02-482/18-25

FL-004712 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 473 – 491 nm
  • Center Wavelength of 482 nm and GMBW of 18 nm
  • FWHM of 24.4 nm

Di01-R405/488/594-25x36

FL-006958 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edges at 420 nm, 497 nm & 603 nm
  • Rabs > 94% 370 – 410 nm, Rabs > 94% 473 – 491 nm & Ravg > 94% 588.3 – 594.3 nm
  • Tavg > 93% 429.5 – 462.0 nm, Tavg > 93% 502.5 – 574.5 nm & Tavg > 93% 612.0 – 800.0 nm

1 of 1

This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-carboxynapthofluorescein (pH 10) 555 615
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
6-carboxyrhodamine 6G 525 555
7-AAD (7-aminoactinomycin D) 548 648
7-hydroxy-4-methylcoumarin 360 442
Acridine Orange (+DNA) 502 526
Alexa Fluor® 405 401 422
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 594 590 618
AMCA (Aminomethylcoumarin) 350 448
AmCyan1 458 489
APC 651 660
APC (Allophycocyanin) 651 660
ATTO 488 500 525
ATTO 495 495 527
ATTO 590 594 624