BrightLine® Sedat laser filter set, optimized for 405, 488, & 594 nm laser sources

Semrock Part Number: LF405/488/594-3X3M-B-OMF

IDEX Part Number: S-001760  /

Semrock

This "Sedat" multiband set is optimized for laser excitation utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, and a 593.5, 594.0 or 594.1 nm laser source for mCherry. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio.

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Laser Wavelength 1 (high) 491 nm, 594.3 nm
Laser Wavelength 1 (low) 473 nm, 593.5 nm
Laser Wavelength 2 488 nm

FF01-647/57-25

FL-004142 / 0

Out of Stock Backordered In Stock
  • Tavg > 92% 618.5 – 675.5 nm
  • Center Wavelength of 647 nm and GMBW of 57 nm
  • FWHM of 70 nm

FF01-445/20-25

FL-004531 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 435 – 455 nm
  • Center Wavelength of 445 nm and GMBW of 20 nm
  • FWHM of 25 nm

FF01-390/40-25

FL-004666 / 0

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  • Tavg > 93% 370 – 410 nm
  • Center Wavelength of 390 nm and GMBW of 40 nm
  • FWHM of 44.6 nm

FF01-525/45-25

FL-004680 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 502.5 – 547.5 nm
  • Center Wavelength of 525 nm and GMBW of 45 nm
  • FWHM of 50.6 nm

FF01-591/6-25

FL-004689 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 588 – 594.5 nm
  • Center Wavelength of 591 nm and GMBW of 6 nm
  • FWHM of 10.0 nm

FF02-482/18-25

FL-004712 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 473 – 491 nm
  • Center Wavelength of 482 nm and GMBW of 18 nm
  • FWHM of 24.4 nm

Di01-R405/488/594-25x36

FL-006958 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edges at 420 nm, 497 nm & 603 nm
  • Rabs > 94% 370 – 410 nm, Rabs > 94% 473 – 491 nm & Ravg > 94% 588.3 – 594.3 nm
  • Tavg > 93% 429.5 – 462.0 nm, Tavg > 93% 502.5 – 574.5 nm & Tavg > 93% 612.0 – 800.0 nm

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This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-carboxynapthofluorescein (pH 10) 555 615
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
6-carboxyrhodamine 6G 525 555
7-AAD (7-aminoactinomycin D) 548 648
7-hydroxy-4-methylcoumarin 360 442
Acridine Orange (+DNA) 502 526
Alexa Fluor® 405 401 422
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 594 590 618
AMCA (Aminomethylcoumarin) 350 448
AmCyan1 458 489
APC 651 660
APC (Allophycocyanin) 651 660
ATTO 488 500 525
ATTO 495 495 527
ATTO 590 594 624