This "Sedat" multiband set is optimized for laser excitation utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, and a 593.5, 594.0 or 594.1 nm laser source for mCherry. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio.
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Laser Wavelength 1 (high) | 491 nm, 594.3 nm |
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Laser Wavelength 1 (low) | 473 nm, 593.5 nm |
Laser Wavelength 2 | 488 nm |
FL-004142 / 0
FL-004531 / 0
FL-004666 / 0
FL-004680 / 0
FL-004689 / 0
FL-004712 / 0
FL-006958 / 0
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This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 3.5 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
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1,8-ANS | 372 | 477 |
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4-methylumbelliferone | 357 | 441 |
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5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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5-FAM (5-carboxyfluorescein) | 492 | 518 |
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5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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6-carboxyrhodamine 6G | 525 | 555 |
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7-AAD (7-aminoactinomycin D) | 548 | 648 |
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7-hydroxy-4-methylcoumarin | 360 | 442 |
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Acridine Orange (+DNA) | 502 | 526 |
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Alexa Fluor® 405 | 401 | 422 |
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Alexa Fluor® 430 | 431 | 541 |
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Alexa Fluor® 488 | 499 | 520 |
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Alexa Fluor® 500 | 503 | 525 |
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Alexa Fluor® 594 | 590 | 618 |
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AMCA (Aminomethylcoumarin) | 350 | 448 |
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AmCyan1 | 458 | 489 |
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APC | 651 | 660 |
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APC (Allophycocyanin) | 651 | 660 |
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ATTO 488 | 500 | 525 |
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ATTO 495 | 495 | 527 |
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ATTO 590 | 594 | 624 |
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