This "Sedat" multiband set is optimized for laser excitation utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, and a 593.5, 594.0 or 594.1 nm laser source for mCherry. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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0.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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| Laser Wavelength 1 (high) | 491 nm, 594.3 nm |
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| Laser Wavelength 1 (low) | 473 nm, 593.5 nm |
| Laser Wavelength 2 | 488 nm |
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FF01-647/57-25
FL-004142 / 0
- Tavg > 92% 618.5 – 675.5 nm
- Center Wavelength of 647 nm and GMBW of 57 nm
- FWHM of 70 nm
FF01-445/20-25
FL-004531 / 0
- Tavg > 93% 435 – 455 nm
- Center Wavelength of 445 nm and GMBW of 20 nm
- FWHM of 25 nm
FF01-390/40-25
FL-004666 / 0
- Tavg > 93% 370 – 410 nm
- Center Wavelength of 390 nm and GMBW of 40 nm
- FWHM of 44.6 nm
FF01-525/45-25
FL-004680 / 0
- Tavg > 93% 502.5 – 547.5 nm
- Center Wavelength of 525 nm and GMBW of 45 nm
- FWHM of 50.6 nm
FF01-591/6-25
FL-004689 / 0
- Tavg > 93% 588 – 594.5 nm
- Center Wavelength of 591 nm and GMBW of 6 nm
- FWHM of 10.0 nm
FF02-482/18-25
FL-004712 / 0
- Tavg > 93% 473 – 491 nm
- Center Wavelength of 482 nm and GMBW of 18 nm
- FWHM of 24.4 nm
Di01-R405/488/594-25x36
FL-006958 / 0
- Laser Dichroic Beamsplitter with edges at 420 nm, 497 nm & 603 nm
- Rabs > 94% 370 – 410 nm, Rabs > 94% 473 – 491 nm & Ravg > 94% 588.3 – 594.3 nm
- Tavg > 93% 429.5 – 462.0 nm, Tavg > 93% 502.5 – 574.5 nm & Tavg > 93% 612.0 – 800.0 nm
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
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| 1,8-ANS | 372 | 477 |
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| 4-methylumbelliferone | 357 | 441 |
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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| 6-carboxyrhodamine 6G | 525 | 555 |
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
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| 7-hydroxy-4-methylcoumarin | 360 | 442 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Alexa Fluor® 405 | 401 | 422 |
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| Alexa Fluor® 430 | 431 | 541 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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| Alexa Fluor® 594 | 590 | 618 |
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| AMCA (Aminomethylcoumarin) | 350 | 448 |
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| AmCyan1 | 458 | 489 |
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| APC | 651 | 660 |
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| APC (Allophycocyanin) | 651 | 660 |
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| ATTO 488 | 500 | 525 |
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| ATTO 495 | 495 | 527 |
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| ATTO 590 | 594 | 624 |
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