This "Sedat" multiband set is optimized for laser excitation utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, and a 593.5, 594.0 or 594.1 nm laser source for mCherry. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio.
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LF405/488/594-3X3M-B-NQF
S-001757 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
LF405/488/594-3X3M-B-NTE
S-001758 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
LF405/488/594-3X3M-B-OMF
S-001760 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
LF405/488/594-3X3M-B-ZHE
S-001761 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
LF405/488/594-3X3M-B-OFF
S-001759 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63
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| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
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| 4-methylumbelliferone | 357 | 441 |
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
|
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| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
|
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
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| 6-carboxyrhodamine 6G | 525 | 555 |
|
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
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| 7-hydroxy-4-methylcoumarin | 360 | 442 |
|
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| Acridine Orange (+DNA) | 502 | 526 |
|
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| Alexa Fluor® 405 | 401 | 422 |
|
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| Alexa Fluor® 430 | 431 | 541 |
|
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| Alexa Fluor® 488 | 499 | 520 |
|
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| Alexa Fluor® 500 | 503 | 525 |
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| Alexa Fluor® 594 | 590 | 618 |
|
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| AMCA (Aminomethylcoumarin) | 350 | 448 |
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| AmCyan1 | 458 | 489 |
|
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| APC | 651 | 660 |
|
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| APC (Allophycocyanin) | 651 | 660 |
|
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| ATTO 488 | 500 | 525 |
|
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| ATTO 495 | 495 | 527 |
|
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| ATTO 590 | 594 | 624 |
|
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| ATTO 594 | 601 | 624 |
|
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| ATTO 610 | 614 | 634 |
|
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| ATTO 620 | 618 | 642 |
|
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| ATTO 635 | 635 | 658 |
|
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| ATTO 647 | 644 | 670 |
|
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| BCECF (pH 5.5) | 481 | 518 |
|
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| BCECF (pH 9.0) | 502 | 528 |
|
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| BD Horizon V450 | 406 | 449 |
|
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| BFP (EBFP) | 380 | 440 |
|
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| BODIPY 630/650-X | 625 | 641 |
|
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| BODIPY FL | 505 | 512 |
|
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| BODIPY FL-MeOH | 502 | 511 |
|
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| BODIPY TR-X phallacidin | 590 | 621 |
|
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| Calcein | 494 | 514 |
|
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| Calcium Crimson | 589 | 609 |
|
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| Calcium Green-1 | 507 | 529 |
|
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| Calcofluor White | 351 | 444 |
|
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| Carboxynaphthofluorescein | 599 | 674 |
|
|
| Cascade Blue™ | 401 | 419 |
|
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| CellTrace BODIPY TR methyl ester | 598 | 625 |
|
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| CellTrace calcein violet | 400 | 452 |
|
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| CellTracker Red CMTPX | 586 | 614 |
|
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| CFP2 | 490 | 510 |
|
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| ChlorophyllA | 480 | 650 |
|
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| Cy2™ | 492 | 507 |
|
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| CyQUANT GR-DNA | 502 | 523 |
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| DAF-FM-NO | 495 | 519 |
|
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| DAPI | 359 | 461 |
|
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| DDAO | 648 | 657 |
|
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| Dendra2 (Green) | 491 | 507 |
|
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| DiA | 457 | 586 |
|
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| DiFMU-pH 9.0 | 358 | 450 |
|
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| DiO | 475 | 500 |
|
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| Dronpa | 502 | 516 |
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| DY-505-Phalloidin | 503 | 530 |
|
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| DyLight 405 | 398 | 420 |
|
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| DyLight 594 | 593 | 618 |
|
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| DyLight 633 | 620 | 649 |
|
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| ecliptic pHluorin pH5.5 | 473 | 507 |
|
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| Emerald | 491 | 511 |
|
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| ER-Tracker™ Blue-White DPX | 372 | 557 |
|
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| evoglow-Bs1 | 449 | 496 |
|
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| FITC (Fluorescein) | 495 | 519 |