BrightLine® Sedat laser filter set, optimized for 405, 488, & 594 nm laser sources

Semrock Part Number: LF405/488/594-3X3M-B-000

IDEX Part Number: S-003072  /

Semrock

This "Sedat" multiband set is optimized for laser excitation utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, and a 593.5, 594.0 or 594.1 nm laser source for mCherry. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio.

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  • Each
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  • Price Not Available.
  • 3,000.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
Set Components

Di01-R405/488/594-25x36

FL-006958

Each
  • Laser Dichroic Beamsplitter with edges at 420 nm, 497 nm & 603 nm
  • Rabs > 94% 370 – 410 nm, Rabs > 94% 473 – 491 nm & Ravg > 94% 588.3 – 594.3 nm
  • Tavg > 93% 429.5 – 462.0 nm, Tavg > 93% 502.5 – 574.5 nm & Tavg > 93% 612.0 – 800.0 nm

FL-006958

FF01-390/40-25

FL-004666

Each
  • Tavg > 93% 370 – 410 nm
  • Center Wavelength of 390 nm and GMBW of 40 nm
  • FWHM of 44.6 nm

FL-004666

FF01-445/20-25

FL-004531

Each
  • Tavg > 93% 435 – 455 nm
  • Center Wavelength of 445 nm and GMBW of 20 nm
  • FWHM of 25 nm

FL-004531

FF01-525/45-25

FL-004680

Each
  • Tavg > 93% 502.5 – 547.5 nm
  • Center Wavelength of 525 nm and GMBW of 45 nm
  • FWHM of 50.6 nm

FL-004680

FF01-591/6-25

FL-004689

Each
  • Tavg > 93% 588 – 594.5 nm
  • Center Wavelength of 591 nm and GMBW of 6 nm
  • FWHM of 10.0 nm

FL-004689

FF01-647/57-25

FL-004142

Each
  • Tavg > 92% 618.5 – 675.5 nm
  • Center Wavelength of 647 nm and GMBW of 57 nm
  • FWHM of 70 nm

FL-004142

FF02-482/18-25

FL-004712

Each
  • Tavg > 93% 473 – 491 nm
  • Center Wavelength of 482 nm and GMBW of 18 nm
  • FWHM of 24.4 nm

FL-004712

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
Center Wavelength 1 375 nm
Edge Wavelength 1 405 nm
Laser Wavelength 1 (high) 491 nm, 594.3 nm
Laser Wavelength 1 (low) 473 nm, 593.5 nm
Laser Wavelength 2 488 nm
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

LF405/488/594-3X3M-B-NQF

S-001757 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

LF405/488/594-3X3M-B-NTE

S-001758 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

LF405/488/594-3X3M-B-OMF

S-001760 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

LF405/488/594-3X3M-B-ZHE

S-001761 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

LF405/488/594-3X3M-B-OFF

S-001759 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

LF405/488/594-3X3M-B-LDMK

S-001755 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6

LF405/488/594-3X3M-B-LDMP

S-001756 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Leica DMi8 (P-Cube) for microscopes: DMi8