This "Pinkel" multiband set is optimized for laser excitation over four bands - utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, a 559, 561.4 or 568.2 nm laser source for mCherry, and a 632.8, 635, or 647.1 nm laser source for Cy5. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio.
Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF405/488/561/635-4X-B
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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0.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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| Laser Wavelength 1 (high) | 491 nm, 568.2 nm |
|---|---|
| Laser Wavelength 1 (low) | 473 nm, 632.8 nm, 647.1 nm |
| Laser Wavelength 2 | 488 nm |
| Laser Wavelength 3 | 561.4 nm |
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FF01-446/523/600/677-25
FL-004532 / 0
- Tavg > 90% 429.5 – 462.0 nm, Tavg > 90% 502.5 – 544.5 nm, Tavg > 90% 582 – 617.5 nm & Tavg > 90% 663 – 690.5 nm
- Center Wavelengths of 446 nm, 523.5 nm, 600 nm & 677 nm and GMBWs of 32.5 nm, 42 nm, 35.5 nm & 27.5 nm
FF01-390/40-25
FL-004666 / 0
- Tavg > 93% 370 – 410 nm
- Center Wavelength of 390 nm and GMBW of 40 nm
- FWHM of 44.6 nm
FF01-563/9-25
FL-004686 / 0
- Tavg > 93% 559 – 568 nm
- Center Wavelength of 563.5 nm and GMBW of 9 nm
- FWHM of 14.5 nm
FF01-640/14-25
FL-004697 / 0
- Tavg > 93% 633 – 647 nm
- Center Wavelength of 640 nm and GMBW of 14 nm
- FWHM of 20.2 nm
FF02-482/18-25
FL-004712 / 0
- Tavg > 93% 473 – 491 nm
- Center Wavelength of 482 nm and GMBW of 18 nm
- FWHM of 24.4 nm
Di01-R405/488/561/635-25x36
FL-007046 / 0
• Laser Dichroic Beamsplitter with edges at 426.3 nm, 498.3 nm, 575.4 nm & 655.3 nm
• Rabs > 94% 370 – 410 nm, Rabs > 94% 473 – 491 nm, Rabs > 94% 559 – 568.2 nm & Rabs > 94% 632.8 – 647.1 nm
• Tavg > 93% 429.5 – 462.0 nm, Tavg > 93% 502.5 – 544.5 nm, Tavg > 93% 582 – 617.5 nm & Tavg > 93% 663 – 800 nm
• Flatness / RWE: Laser
• Rabs > 94% 370 – 410 nm, Rabs > 94% 473 – 491 nm, Rabs > 94% 559 – 568.2 nm & Rabs > 94% 632.8 – 647.1 nm
• Tavg > 93% 429.5 – 462.0 nm, Tavg > 93% 502.5 – 544.5 nm, Tavg > 93% 582 – 617.5 nm & Tavg > 93% 663 – 800 nm
• Flatness / RWE: Laser
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This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
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| 2-dodecylresorufin-lipid | 582 | 595 |
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| 4-methylumbelliferone | 357 | 441 |
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
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| 7-hydroxy-4-methylcoumarin | 360 | 442 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Alexa Fluor® 405 | 401 | 422 |
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| Alexa Fluor® 430 | 431 | 541 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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| Alexa Fluor® 546 | 556 | 572 |
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| Alexa Fluor® 555 | 553 | 565 |
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| Alexa Fluor® 568 | 579 | 603 |
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| Alexa Fluor® 594 | 590 | 618 |
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| Alexa Fluor® 633 | 631 | 647 |
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| Alexa Fluor® 635 | 633 | 648 |
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| Alexa Fluor® 647 | 653 | 668 |
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| Alexa Fluor® 660 | 663 | 691 |
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| AMCA (Aminomethylcoumarin) | 350 | 448 |
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| AmCyan1 | 458 | 489 |
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| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
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| APC | 651 | 660 |
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| APC (Allophycocyanin) | 651 | 660 |
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| AsRed 2 | 576 | 592 |
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| ATTO 390 | 392 | 477 |
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| ATTO 488 | 500 | 525 |
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| ATTO 495 | 495 | 527 |
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| ATTO 550 | 553 | 576 |
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| ATTO 565 | 563 | 592 |
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| ATTO 633 | 629 | 658 |
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| ATTO 635 | 635 | 658 |
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| ATTO 647 | 644 | 670 |
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| ATTO 647N | 645 | 668 |
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| ATTO 655 | 663 | 683 |
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| BCECF (pH 5.5) | 481 | 518 |
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| BCECF (pH 9.0) | 502 | 528 |
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| BD Horizon V450 | 406 | 449 |
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| BFP (EBFP) | 380 | 440 |
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| BOBO™-3 | 570 | 605 |
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| BODIPY 650/665-X | 646 | 664 |
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| BODIPY FL | 505 | 512 |
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| BODIPY FL-MeOH | 502 | 511 |
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| BODIPY TMR-X | 544 | 573 |
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| BODIPY TR-X (MeOH) | 544 | 573 |
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| BODIPY TR-X phallacidin | 590 | 621 |
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| Calcein | 494 | 514 |
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| Calcium Crimson | 589 | 609 |
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| Calcium Green-1 | 507 | 529 |
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| Calcium Orange | 589 | 609 |
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