This full-multiband set is optimized for laser excitation over four bands - utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, a 532 nm laser source for TRITC, and a 632.8, 635, or 647.1 nm laser source for Cy5. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio.
Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF405/488/532/635-B
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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2,349.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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0.000.00 -
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| Center Wavelength 2 | 532 nm |
|---|---|
| Laser Wavelength 1 (high) | 491 nm |
| Laser Wavelength 1 (low) | 473 nm, 632.8 nm, 647.1 nm |
| Laser Wavelength 2 | 488 nm |
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Select all 3 products from all pages.
FF01-390/482/532/640-25
FL-004407 / 0
- Tavg > 90% 370 – 410 nm, Tavg > 90% 473 – 491 nm, Tavg > 90% 530.5 – 533.5 nm & Tavg > 90% 632.8 – 647.1 nm
- Center Wavelengths of 390 nm, 482 nm, 532 nm & 640 nm and GMBWs of 40 nm, 18 nm, 3 nm & 14 nm
FF01-446/510/581/703-25
FL-004412 / 0
- Tavg > 93% 429.5 – 462.0 nm, Tavg > 93% 502.5 – 518.5 nm, Tavg > 93% 550– 613 nm & Tavg > 93% 663 – 743 nm
- Center Wavelengths of 446 nm, 510.5 nm, 581.5 nm & 703 nm and GMBWs of 32.5 nm, 16 nm, 63 nm & 80 nm
Di01-R405/488/532/635-25x36
FL-006636 / 0
- Laser Dichroic Beamsplitter with edges at 422.3 nm, 497.8 nm, 541.6 nm & 655.9 nm
- Rabs > 94% 370 – 410 nm, Rabs > 94% 473 – 491 nm, Ravg > 94% 530.5 – 533.5 nm & Ravg > 94% 632.8 – 647.1 nm
- Tavg > 93% 429.5 – 462.0 nm, Tavg > 93% 502.5 – 518.5 nm, Tavg > 93% 550 – 613 nm & Tavg > 93% 663 – 800 nm
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
|
|
| 2-dodecylresorufin-lipid | 582 | 595 |
|
|
| 4-methylumbelliferone | 357 | 441 |
|
|
| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
|
|
| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
|
|
| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
|
| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
|
| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
|
|
| 7-hydroxy-4-methylcoumarin | 360 | 442 |
|
|
| Acridine Orange (+DNA) | 502 | 526 |
|
|
| Alexa Fluor® 405 | 401 | 422 |
|
|
| Alexa Fluor® 430 | 431 | 541 |
|
|
| Alexa Fluor® 488 | 499 | 520 |
|
|
| Alexa Fluor® 500 | 503 | 525 |
|
|
| Alexa Fluor® 546 | 556 | 572 |
|
|
| Alexa Fluor® 555 | 553 | 565 |
|
|
| Alexa Fluor® 568 | 579 | 603 |
|
|
| Alexa Fluor® 594 | 590 | 618 |
|
|
| Alexa Fluor® 633 | 631 | 647 |
|
|
| Alexa Fluor® 635 | 633 | 648 |
|
|
| Alexa Fluor® 647 | 653 | 668 |
|
|
| Alexa Fluor® 660 | 663 | 691 |
|
|
| AMCA (Aminomethylcoumarin) | 350 | 448 |
|
|
| AmCyan1 | 458 | 489 |
|
|
| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
|
|
| APC | 651 | 660 |
|
|
| APC (Allophycocyanin) | 651 | 660 |
|
|
| AsRed 2 | 576 | 592 |
|
|
| ATTO 390 | 392 | 477 |
|
|
| ATTO 488 | 500 | 525 |
|
|
| ATTO 495 | 495 | 527 |
|
|
| ATTO 550 | 553 | 576 |
|
|
| ATTO 565 | 563 | 592 |
|
|
| ATTO 633 | 629 | 658 |
|
|
| ATTO 635 | 635 | 658 |
|
|
| ATTO 647 | 644 | 670 |
|
|
| ATTO 647N | 645 | 668 |
|
|
| ATTO 655 | 663 | 683 |
|
|
| BCECF (pH 5.5) | 481 | 518 |
|
|
| BCECF (pH 9.0) | 502 | 528 |
|
|
| BD Horizon V450 | 406 | 449 |
|
|
| BFP (EBFP) | 380 | 440 |
|
|
| BOBO™-3 | 570 | 605 |
|
|
| BODIPY 650/665-X | 646 | 664 |
|
|
| BODIPY FL | 505 | 512 |
|
|
| BODIPY FL-MeOH | 502 | 511 |
|
|
| BODIPY TMR-X | 544 | 573 |
|
|
| BODIPY TR-X (MeOH) | 544 | 573 |
|
|
| BODIPY TR-X phallacidin | 590 | 621 |
|
|
| Calcein | 494 | 514 |
|
|
| Calcium Crimson | 589 | 609 |
|
|
| Calcium Green-1 | 507 | 529 |
|
|
| Calcium Orange | 589 | 609 |
|
|
| Calcofluor White | 351 | 444 |
|
|
| Carboxynaphthofluorescein | 599 | 674 |
|
|
| Cascade Blue™ | 401 | 419 |
|
|
| CellTrace calcein violet | 400 | 452 |
|
|
| CellTracker Red CMTPX | 586 | 614 |
|
|
| CFP2 | 490 | 510 |
|
|
| Cy2™ | 492 | 507 |
|
|
| Cy3.5™ | 578 | 591 |
|
|
| Cy3™ | 554 | 566 |
|
|
| Cy5.5™ | 672 | 690 |
|
|
| Cy5™ | 649 | 666 |
|
|
| CyQUANT GR-DNA | 502 | 523 |
|
|
| DAF-FM-NO | 495 | 519 |
|
|
| DAPI | 359 | 461 |
|
|
| DDAO | 648 | 657 |
|
|
| Dendra2 (Green) | 491 | 507 |
|
|
| Dendra2 (Red) | 554 | 569 |
|
|
| DiD (DiIC18(5)) - Lipophilic Tracer | 648 | 670 |
|
|
| DiFMU-pH 9.0 | 358 | 450 |
|
|
| DiI | 551 | 569 |
|
|
| DiO | 475 | 500 |
|
|
| Dronpa | 502 | 516 |
|
|
| DsRed | 559 | 583 |
|
|
| DsRed-Express | 556 | 584 |
|
|
| dTomato | 556 | 582 |
|
|
| DY-415 | 418 | 470 |
|
|
| DY-505-Phalloidin | 503 | 530 |
|
|
| DY-590 | 582 | 599 |
|
|
| DY-630 | 634 | 656 |
|
|
| DyLight 405 | 398 | 420 |
|
|
| DyLight 594 | 593 | 618 |
|
|
| DyLight 633 | 620 | 649 |
|
|
| DyLight 649 | 651 | 673 |
|
|
| ecliptic pHluorin pH5.5 | 473 | 507 |
|
|
| Emerald | 491 | 511 |
|
|
| ER-Tracker™ Blue-White DPX | 372 | 557 |
|
|
| Ethidium bromide | 518 | 603 |
|
|
| Ethidium homodimer | 527 | 617 |
|
|
| evoglow-Bs1 | 449 | 496 |
|
|
| FITC (Fluorescein) | 495 | 519 |
|
|
| FlAsH-CCPFCC | 511 | 530 |
|
|
| Fluo-3 | 506 | 527 |
|
|
| Fluo-4 | 494 | 516 |
|
|
| Fluorescein dextran | 501 | 524 |
|
|
| Fluorescein-pH 8.0 | 489 | 517 |
|
|
| Fluoro-Emerald | 494 | 518 |
|
|
| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
|
|
| FluoSpheres Dark Red fluorescent microspheres | 656 | 683 |
|
|
| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
|
| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
|
| FM 1-43 | 473 | 579 |
|
|
| GFP (EGFP) | 489 | 511 |
|
|
| Green 496 | 496 | 520 |
|
|
| Green 500 | 501 | 524 |
|
|
| HCS CellMask Red cytoplasmic | 622 | 644 |
|
|
| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
|
|
| HCS LipidTOX Green neutral lipid stain | 498 | 507 |
|
|
| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
|
| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
|
| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
|
| HiLyte Fluor™ 405 | 403 | 428 |
|
|
| HiLyte Fluor™ 488 | 497 | 526 |
|
|
| HiLyte Fluor™ 594 | 592 | 616 |
|
|
| HiLyte Fluor™ 647 | 649 | 673 |
|
|
| Hoechst 33258 | 352 | 455 |
|
|
| Hypericin | 600 | 603 |
|
|
| KFP-Red | 575 | 599 |
|
|
| LIVE-DEAD Fixable Far Red Dead Cell Stain | 653 | 668 |
|
|
| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
|
| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
|
|
| LOLO-1 | 568 | 580 |
|
|
| LysoSensor Blue | 374 | 424 |
|
|
| LysoSensor Green | 448 | 502 |
|
|
| LysoTracker Blue | 373 | 422 |
|
|
| LysoTracker Green | 501 | 509 |
|
|
| LysoTracker Red | 573 | 592 |
|
|
| LysoTracker Yellow HCK-123 | 488 | 565 |
|
|
| Magnesium Green | 507 | 531 |
|
|
| Magnesium Orange | 550 | 574 |
|
|
| mApple | 566 | 594 |
|
|
| Marina Blue | 363 | 461 |
|
|
| mCherry | 587 | 610 |
|
|
| Merocyanine 540 | 559 | 579 |
|
|
| mHoneyDew | 478 | 562 |
|
|
| MitoTracker™ Deep Red | 641 | 662 |
|
|
| MitoTracker™ Green | 490 | 512 |
|
|
| MitoTracker™ Orange | 551 | 575 |
|
|
| MitoTracker™ Red | 578 | 598 |
|
|
| mKate2 | 588 | 633 |
|
|
| mOrange2 | 551 | 565 |
|
|
| mRFP | 555 | 583 |
|
|
| mRFP1 | 584 | 700 |
|
|
| mRuby | 558 | 605 |
|
|
| mStrawberry | 574 | 596 |
|
|
| mTangerine | 568 | 585 |
|
|
| mWasabi | 493 | 509 |
|
|
| NBD-X (MeOH) | 467 | 538 |
|
|
| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
|
| Nile blue-EtOH | 631 | 660 |
|
|
| Nile red-phospolipid | 553 | 637 |
|
|
| Orange 552 | 552 | 574 |
|
|
| Oregon Green™ 488 | 498 | 526 |
|
|
| Oregon Green™ 514 | 513 | 532 |
|
|
| Pacific Blue™ | 404 | 455 |
|
|
| pHrodo™, succinimidyl ester | 560 | 587 |
|
|
| PicoGreen® | 502 | 522 |
|
|
| PKH67 | 488 | 500 |
|
|
| Propidium Iodide (PI) | 305 | 617 |
|
|
| Pro-Q Diamond | 556 | 583 |
|
|
| Qdot® 525 Nanocrystals | 300 | 525 |
|
|
| Qdot® 545 Nanocrystals | 300 | 543 |
|
|
| Qdot® 585 Nanocrystals | 300 | 588 |
|
|
| Qdot® 605 Nanocrystals | 300 | 602 |
|
|
| Qdot® 705 Nanocrystals | 300 | 702 |
|
|
| ratiometric pHluorin pH5 | 500 | 509 |
|
|
| ReAsH-CCPGCC | 592 | 606 |
|
|
| Red 580 | 580 | 603 |
|
|
| Red 650 | 649 | 665 |
|
|
| Resorufin | 571 | 584 |
|
|
| Rhod-2 | 553 | 577 |
|
|
| Rhodamine 110 | 497 | 519 |
|
|
| Rhodamine 123 | 507 | 529 |
|
|
| Rhodamine Green | 497 | 523 |
|
|
| Rhodamine phalloidin | 558 | 575 |
|
|
| Rhodamine Red-X | 573 | 591 |
|
|
| Rhodol Green | 497 | 524 |
|
|
| R-phycoerythrin (PE) | 565 | 576 |
|
|
| sgBFP™ | 387 | 451 |
|
|
| sgGFP™ (super glow GFP) | 472 | 506 |
|
|
| Sirius | 355 | 424 |
|
|
| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
|
| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
|
| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
|
| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
|
| SNARF-1 514nm (ph 9.0) | 576 | 638 |
|
|
| Sodium Green | 507 | 532 |
|
|
| SpectrumBlue | 405 | 449 |
|
|
| SpectrumFRed (Far Red) | 650 | 673 |
|
|
| SpectrumGreen | 497 | 538 |
|
|
| SpectrumOrange | 554 | 587 |
|
|
| Sulforhodamine 101-EtOH | 578 | 593 |
|
|
| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
|
| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
|
| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
|
| SYTO 11 | 506 | 525 |
|
|
| SYTO 13 | 448 | 506 |
|
|
| SYTO 16 | 489 | 520 |
|
|
| SYTO 59 | 622 | 644 |
|
|
| SYTO 60 | 650 | 681 |
|
|
| SYTO 61 | 618 | 650 |
|
|
| SYTO 9 | 483 | 500 |
|
|
| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
|
| SYTOX Green-DNA | 504 | 524 |
|
|
| SYTOX Orange | 547 | 570 |
|
|
| SYTOX Red-DNA | 640 | 658 |
|
|
| TagBFP |