BrightLine® full-multiband laser filter set, optimized for 405, 488, 532, & 635 nm laser sources

Semrock Part Number: LF405/488/532/635-A-000

IDEX Part Number: S-000876  /

Semrock

This full-multiband set is optimized for laser excitation over four bands - utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, a 532 nm laser source for TRITC, and a 632.8, 635, or 647.1 nm laser source for Cy5. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio.

Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF405/488/532/635-B

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  • 1,940.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
Set Components

Di01-R405/488/532/635-25x36

FL-006636

Each
  • Laser Dichroic Beamsplitter with edges at 422.3 nm, 497.8 nm, 541.6 nm & 655.9 nm
  • Rabs > 94% 370 – 410 nm, Rabs > 94% 473 – 491 nm, Ravg > 94% 530.5 – 533.5 nm & Ravg > 94% 632.8 – 647.1 nm
  • Tavg > 93% 429.5 – 462.0 nm, Tavg > 93% 502.5 – 518.5 nm, Tavg > 93% 550 – 613 nm & Tavg > 93% 663 – 800 nm

FL-006636

FF01-390/482/532/640-25

FL-004407

Each
  • Tavg > 90% 370 – 410 nm, Tavg > 90% 473 – 491 nm, Tavg > 90% 530.5 – 533.5 nm & Tavg > 90% 632.8 – 647.1 nm
  • Center Wavelengths of 390 nm, 482 nm, 532 nm & 640 nm and GMBWs of 40 nm, 18 nm, 3 nm & 14 nm

FL-004407

FF01-446/510/581/703-25

FL-004412

Each
  • Tavg > 93% 429.5 – 462.0 nm, Tavg > 93% 502.5 – 518.5 nm, Tavg > 93% 550– 613 nm & Tavg > 93% 663 – 743 nm
  • Center Wavelengths of 446 nm, 510.5 nm, 581.5 nm & 703 nm and GMBWs of 32.5 nm, 16 nm, 63 nm & 80 nm

FL-004412

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
Center Wavelength 1 375 nm
Center Wavelength 4 635 nm, 640 nm
Edge Wavelength 1 405 nm, 638 nm
Laser Wavelength 1 (high) 491 nm
Laser Wavelength 1 (low) 473 nm, 532 nm, 632.8 nm, 647.1 nm
Laser Wavelength 2 488 nm
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

LF405/488/532/635-B-000

S-000877 / 0

Out of Stock Backordered In Stock
1λ P-V RWE super-resolution laser optimized multiband set with quad-band exciter, emitter, and beamsplitter filters. Ideal for use with: Blue: DAPI; Green: FITC , GFP ; Orange: TRITC; Red: Cy5

LF405/488/532/635-A-NQF

S-001721 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

LF405/488/532/635-A-NTE

S-001722 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

LF405/488/532/635-A-OMF

S-001089 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

LF405/488/532/635-A-ZHE

S-001724 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

LF405/488/532/635-A-OFF

S-001723 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

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TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
2-dodecylresorufin-lipid 582 595
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-carboxynapthofluorescein (pH 10) 555 615
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
7-hydroxy-4-methylcoumarin 360 442
Acridine Orange (+DNA) 502 526
Alexa Fluor® 405 401 422
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 546 556 572
Alexa Fluor® 555 553 565
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
Alexa Fluor® 633 631 647
Alexa Fluor® 635 633 648
Alexa Fluor® 647 653 668
Alexa Fluor® 660 663 691
AMCA (Aminomethylcoumarin) 350 448
AmCyan1 458 489
Amplex UltraRed peroxidation product (pH 7.5) 568 581
APC 651 660
APC (Allophycocyanin) 651 660
AsRed 2 576 592
ATTO 390 392 477