BrightLine® Pinkel laser filter set, optimized for 405, 488, 532, & 635 nm laser sources

Semrock Part Number: LF405/488/532/635-4X-A-LDMP

IDEX Part Number: S-001713  /

Semrock

This "Pinkel" multiband set is optimized for laser excitation over four bands - utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, a 532 nm laser source for TRITC, and a 632.8, 635, or 647.1 nm laser source for Cy5. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio.

Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF405/488/532/635-4X-B

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  • Price Not Available.
  • 3,429.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Center Wavelength 2 532 nm
Laser Wavelength 1 (high) 491 nm
Laser Wavelength 1 (low) 473 nm, 632.8 nm, 647.1 nm
Laser Wavelength 2 488 nm

FF01-532/3-25

FL-004387 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 530.5 – 533.5 nm
  • Center Wavelength of 532 nm and GMBW of 3 nm
  • FWHM of 9.2 nm

FF01-446/510/581/703-25

FL-004412 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 429.5 – 462.0 nm, Tavg > 93% 502.5 – 518.5 nm, Tavg > 93% 550– 613 nm & Tavg > 93% 663 – 743 nm
  • Center Wavelengths of 446 nm, 510.5 nm, 581.5 nm & 703 nm and GMBWs of 32.5 nm, 16 nm, 63 nm & 80 nm

FF01-390/40-25

FL-004666 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 370 – 410 nm
  • Center Wavelength of 390 nm and GMBW of 40 nm
  • FWHM of 44.6 nm

FF01-640/14-25

FL-004697 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 633 – 647 nm
  • Center Wavelength of 640 nm and GMBW of 14 nm
  • FWHM of 20.2 nm

FF02-482/18-25

FL-004712 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 473 – 491 nm
  • Center Wavelength of 482 nm and GMBW of 18 nm
  • FWHM of 24.4 nm

Di01-R405/488/532/635-25x36

FL-006636 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edges at 422.3 nm, 497.8 nm, 541.6 nm & 655.9 nm
  • Rabs > 94% 370 – 410 nm, Rabs > 94% 473 – 491 nm, Ravg > 94% 530.5 – 533.5 nm & Ravg > 94% 632.8 – 647.1 nm
  • Tavg > 93% 429.5 – 462.0 nm, Tavg > 93% 502.5 – 518.5 nm, Tavg > 93% 550 – 613 nm & Tavg > 93% 663 – 800 nm

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This set is mounted in a Leica Microsystems DMi8 (“P-Cube”) Fluorescence Filter Holder, which is compatible with the DMi8 Leica microscope.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
2-dodecylresorufin-lipid 582 595
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-carboxynapthofluorescein (pH 10) 555 615
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
7-hydroxy-4-methylcoumarin 360 442
Acridine Orange (+DNA) 502 526
Alexa Fluor® 405 401 422
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 546 556 572
Alexa Fluor® 555 553 565
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
Alexa Fluor® 633 631 647
Alexa Fluor® 635 633 648
Alexa Fluor® 647 653 668
Alexa Fluor® 660 663 691
AMCA (Aminomethylcoumarin) 350 448
AmCyan1 458 489
Amplex UltraRed peroxidation product (pH 7.5) 568 581
APC 651 660
APC (Allophycocyanin) 651 660
AsRed 2 576 592
ATTO 390 392 477
ATTO 488 500 525
ATTO 495 495 527
ATTO 550 553 576
ATTO 565 563 592
ATTO 633 629 658
ATTO 635 635 658
ATTO 647 644 670
ATTO 647N 645 668
ATTO 655 663 683
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BD Horizon V450 406 449
BFP (EBFP) 380 440
BOBO™-3 570 605
BODIPY 650/665-X 646 664
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
BODIPY TMR-X 544 573
BODIPY TR-X (MeOH) 544 573
BODIPY TR-X phallacidin 590 621
Calcein 494 514
Calcium Crimson 589 609
Calcium Green-1 507 529
Calcium Orange 589 609
Calcofluor White 351 444
Carboxynaphthofluorescein 599 674
Cascade Blue™ 401 419
CellTrace calcein violet 400 452
CellTracker Red CMTPX 586 614
CFP2 490 510
Cy2™ 492 507
Cy3.5™ 578 591
Cy3™ 554 566
Cy5.5™ 672 690