Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.
This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring YFP (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market.
Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,648.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-509/22-25
FL-004258 / 0
- Tavg > 93% 497.6 – 519.5 nm
- Center Wavelength of 508.6 nm and GMBW of 21.9 nm
- FWHM of 26.1 nm
FF01-544/24-25
FL-004684 / 0
- Tavg > 93% 532 – 556 nm
- Center Wavelength of 544 nm and GMBW of 24 nm
- FWHM of 29.1 nm
FF526-Di01-25x36
FL-007012 / 0
- Dichroic Beamsplitter with edge at 526 nm
- Ravg > 98% 350 – 519.5 nm
- Tavg > 93% 532 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
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This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 6-carboxyrhodamine 6G | 525 | 555 |
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| 6-JOE | 520 | 548 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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| Alexa Fluor® 514 | 518 | 543 |
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| Alexa Fluor® 532 | 534 | 553 |
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| ATTO 488 | 500 | 525 |
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| ATTO 495 | 495 | 527 |
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| ATTO 520 | 516 | 538 |
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| ATTO 532 | 501 | 545 |
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| BODIPY R6G | 528 | 547 |
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| Calcein | 494 | 514 |
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| Calcium Green-1 | 507 | 529 |
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| CyQUANT GR-DNA | 502 | 523 |
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| DAF-FM-NO | 495 | 519 |
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| Dronpa | 502 | 516 |
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| DY-505-Phalloidin | 503 | 530 |
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| DyLight 488 | 493 | 518 |
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| Eosin | 525 | 546 |
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| FITC (Fluorescein) | 495 | 519 |
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| FlAsH-CCPFCC | 511 | 530 |
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| Fluo-3 | 506 | 527 |
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| Fluo-4 | 494 | 516 |
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| Fluorescein dextran | 501 | 524 |
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| Fluorescein-pH 8.0 | 489 | 517 |
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| Fluoro-Emerald | 494 | 518 |
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| FluoSpheres Orange fluorescent microspheres | 540 | 559 |
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
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| FM 1-43 | 473 | 579 |
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| Gold 525 | 521 | 551 |
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| Green 496 | 496 | 520 |
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| Green 500 | 501 | 524 |
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
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| HiLyte Fluor™ 488 | 497 | 526 |
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| JO-JO-1, JO-PRO-1 | 530 | 544 |
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
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| LysoTracker Yellow HCK-123 | 488 | 565 |
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| Magnesium Green | 507 | 531 |
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| mHoneyDew | 478 | 562 |
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| mOrange2 | 551 | 565 |
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
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| Oregon Green™ 488 | 498 | 526 |
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| Oregon Green™ 514 | 513 | 532 |
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| PicoGreen® | 502 | 522 |
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| Qdot® 525 Nanocrystals | 300 | 525 |
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| Qdot® 545 Nanocrystals | 300 | 543 |
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| Rhodamine 110 | 497 | 519 |
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| Rhodamine 123 | 507 | 529 |
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| Rhodamine Green | 497 | 523 |
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| Rhodol Green | 497 | 524 |
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| Sodium Green | 507 | 532 |
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| SpectrumGreen | 497 | 538 |
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
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| SYTO 11 | 506 | 525 |
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
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| SYTOX Green-DNA | 504 | 524 |
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| TO-PRO-1 | 515 | 531 |
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| TOTO-1 | 514 | 531 |
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| TurboYFP | 525 | 538 |
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| Venus | 516 | 528 |
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| VIC | 530 | 550 |
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| Vybrant DyeCycle Green | 506 | 534 |
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| Vybrant DyeCycle Orange | 519 | 563 |
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| YFP (yellow GFP) | 513 | 530 |
|
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| ZsYellow1 | 529 | 539 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-509/22-25
FL-004258 / 0
- Tavg > 93% 497.6 – 519.5 nm
- Center Wavelength of 508.6 nm and GMBW of 21.9 nm
- FWHM of 26.1 nm
FF01-544/24-25
FL-004684 / 0
- Tavg > 93% 532 – 556 nm
- Center Wavelength of 544 nm and GMBW of 24 nm
- FWHM of 29.1 nm
FF526-Di01-25x36
FL-007012 / 0
- Dichroic Beamsplitter with edge at 526 nm
- Ravg > 98% 350 – 519.5 nm
- Tavg > 93% 532 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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