BrightLine® single-band filter set, optimized for YFP and other like fluorophores, illuminated with LED-based light sources

Semrock Part Number: LED-YFP-A-000-ZERO

IDEX Part Number: S-000873  /

Semrock
High brightness and contrast single-band filter set. Ideal for use with: YFP (yellow GFP), Venus, Alexa Fluor 514™, Fluo-3, 6-JOE, Gold 525, TO-PRO-1
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Filter set mounted in microscope holders/cubes found below in related products section.
Set Components

FF01-509/22-25

FL-004258

Each
  • Tavg > 93% 497.6 – 519.5 nm
  • Center Wavelength of 508.6 nm and GMBW of 21.9 nm
  • FWHM of 26.1 nm

FL-004258

FF01-544/24

FL-003615

Each

FL-003615

FF526-Di01

FL-006649

Each

FL-006649

Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
6-carboxyrhodamine 6G 525 555
6-JOE 520 548
Acridine Orange (+DNA) 502 526
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 514 518 543
Alexa Fluor® 532 534 553
ATTO 488 500 525
ATTO 495 495 527
ATTO 520 516 538
ATTO 532 501 545
BODIPY R6G 528 547
Calcein 494 514
Calcium Green-1 507 529
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
Dronpa 502 516
DY-505-Phalloidin 503 530
DyLight 488 493 518
Eosin 525 546
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Orange fluorescent microspheres 540 559
FluoSpheres Yellow-Green fluorescent microspheres 503 514
FM 1-43 473 579
Gold 525 521 551
Green 496 496 520
Green 500 501 524
HCS LipidTOX Green phospholipidosis 504 536
HiLyte Fluor™ 488 497 526
JO-JO-1, JO-PRO-1 530 544
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
mHoneyDew 478 562
mOrange2 551 565
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Oregon Green™ 488 498 526
Oregon Green™ 514 513 532
PicoGreen® 502 522
Qdot® 525 Nanocrystals 300 525
Qdot® 545 Nanocrystals 300 543
Rhodamine 110 497 519
Rhodamine 123 507 529
Rhodamine Green 497 523
Rhodol Green 497 524
Sodium Green 507 532
SpectrumGreen 497 538
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYTO 11 506 525
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
TO-PRO-1 515 531
TOTO-1 514 531
TurboYFP 525 538
Venus 516 528
VIC 530 550
Vybrant DyeCycle Green 506 534
Vybrant DyeCycle Orange 519 563
YFP (yellow GFP) 513 530
ZsYellow1 529 539

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