BrightLine® single-band filter set, optimized for YFP and other like fluorophores, illuminated with LED based light sources

Semrock Part Number: LED-YFP-A-000

IDEX Part Number: S-000872  /

Semrock

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring YFP (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

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  • Price Not Available.
  • 1,130.00 USD Each
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Filter set mounted in microscope holders/cubes found below in related products section.
Set Components

FF01-509/22-25

FL-004258

Each
  • Tavg > 93% 497.6 – 519.5 nm
  • Center Wavelength of 508.6 nm and GMBW of 21.9 nm
  • FWHM of 26.1 nm

FL-004258

FF01-544/24-25

FL-004684

Each
  • Tavg > 93% 532 – 556 nm
  • Center Wavelength of 544 nm and GMBW of 24 nm
  • FWHM of 29.1 nm

FL-004684

FF526-Di01-25x36

FL-007012

Each
  • Dichroic Beamsplitter with edge at 526 nm
  • Ravg > 98% 350 – 519.5 nm
  • Tavg > 93% 532 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

FL-007012

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMakerâ„¢ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

LED-YFP-A-U03

S-001130 / 0

Out of Stock Backordered In Stock
This set is delivered unmounted and fits Olympus OFF cubes which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

LED-YFP-A-NQF

S-000420 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

LED-YFP-A-NTE

S-000421 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

LED-YFP-A-OMF

S-000424 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

LED-YFP-A-ZHE

S-000426 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

LED-YFP-A-OFF

S-000423 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

LED-YFP-A-000-ZERO

S-000873 / 0

Out of Stock Backordered In Stock

Standard size -ZERO pixel set

LED-YFP-A-NQF-ZERO

S-001705 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series