This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring Venus (or other key fluorophores – see complete list above). This state-of-the-art set has been optimized for popular LED based light sources. This set has been designed to deliver significant fluorescence signal improvements for LED based light sources compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps.
Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 5.0 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
---|---|---|---|---|
5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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5-FAM (5-carboxyfluorescein) | 492 | 518 |
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6-carboxyrhodamine 6G | 525 | 555 |
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6-JOE | 520 | 548 |
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Acridine Orange (+DNA) | 502 | 526 |
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Alexa Fluor® 488 | 499 | 520 |
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Alexa Fluor® 500 | 503 | 525 |
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Alexa Fluor® 514 | 518 | 543 |
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Alexa Fluor® 532 | 534 | 553 |
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ATTO 488 | 500 | 525 |
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ATTO 495 | 495 | 527 |
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ATTO 520 | 516 | 538 |
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ATTO 532 | 501 | 545 |
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BODIPY R6G | 528 | 547 |
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Calcein | 494 | 514 |
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Calcium Green-1 | 507 | 529 |
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CyQUANT GR-DNA | 502 | 523 |
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DAF-FM-NO | 495 | 519 |
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Dronpa | 502 | 516 |
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DY-505-Phalloidin | 503 | 530 |
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DyLight 488 | 493 | 518 |
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Eosin | 525 | 546 |
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FITC (Fluorescein) | 495 | 519 |
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FlAsH-CCPFCC | 511 | 530 |
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Fluo-3 | 506 | 527 |
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Fluo-4 | 494 | 516 |
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Fluorescein dextran | 501 | 524 |
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Fluorescein-pH 8.0 | 489 | 517 |
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Fluoro-Emerald | 494 | 518 |
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FluoSpheres Orange fluorescent microspheres | 540 | 559 |
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FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
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FM 1-43 | 473 | 579 |
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Gold 525 | 521 | 551 |
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Green 496 | 496 | 520 |
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Green 500 | 501 | 524 |
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HCS LipidTOX Green phospholipidosis | 504 | 536 |
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HiLyte Fluorâ„¢ 488 | 497 | 526 |
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JO-JO-1, JO-PRO-1 | 530 | 544 |
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LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
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LysoTracker Yellow HCK-123 | 488 | 565 |
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Magnesium Green | 507 | 531 |
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mHoneyDew | 478 | 562 |
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mOrange2 | 551 | 565 |
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NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
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Oregon Greenâ„¢ 488 | 498 | 526 |
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Oregon Greenâ„¢ 514 | 513 | 532 |
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PicoGreen® | 502 | 522 |
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Qdot® 525 Nanocrystals | 300 | 525 |
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Qdot® 545 Nanocrystals | 300 | 543 |
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Rhodamine 110 | 497 | 519 |
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Rhodamine 123 | 507 | 529 |
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Rhodamine Green | 497 | 523 |
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Rhodol Green | 497 | 524 |
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Sodium Green | 507 | 532 |
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SpectrumGreen | 497 | 538 |
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SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
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SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
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SYBR Safe DNA gel stain-DNA | 509 | 526 |
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SYTO 11 | 506 | 525 |
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SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
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SYTOX Green-DNA | 504 | 524 |
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TO-PRO-1 | 515 | 531 |
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TOTO-1 | 514 | 531 |
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TurboYFP | 525 | 538 |
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Venus | 516 | 528 |
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VIC | 530 | 550 |
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Vybrant DyeCycle Green | 506 | 534 |
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Vybrant DyeCycle Orange | 519 | 563 |
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YFP (yellow GFP) | 513 | 530 |
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ZsYellow1 | 529 | 539 |
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This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 5.0 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |