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BrightLine® single-band filter set, optimized for Venus and other like fluorophores, illuminated with LED-based light sources

Semrock Part Number: LED-Venus-A-000

IDEX Part Number: S-000870  /

Semrock

This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring Venus (or other key fluorophores – see complete list above). This state-of-the-art set has been optimized for popular LED based light sources. This set has been designed to deliver significant fluorescence signal improvements for LED based light sources compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,175.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
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Set Components

FF01-513/13-25

FL-003777

Each
  • Tavg > 93% 506.5 – 519.5 nm
  • Center Wavelength of 513 nm and GMBW of 13 nm
  • FWHM of 17.5 nm

FL-003777

FF01-544/24-25

FL-004684

Each
  • Tavg > 93% 532 – 556 nm
  • Center Wavelength of 544 nm and GMBW of 24 nm
  • FWHM of 29.1 nm

FL-004684

FF526-Di01-25x36

FL-007012

Each
  • Dichroic Beamsplitter with edge at 526 nm
  • Ravg > 98% 350 – 519.5 nm
  • Tavg > 93% 532 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

FL-007012

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

LED-Venus-A-OMF

S-002998 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

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LED-Venus-A-ZHE

S-003001 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

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LED-Venus-A-OFF

S-002995 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

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LED-Venus-A-000-ZERO

S-000871 / 0

Out of Stock Backordered In Stock

Standard size -ZERO pixel set

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LED-Venus-A-ZHE-ZERO

S-003002 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

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TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
6-carboxyrhodamine 6G 525 555
6-JOE 520 548
Acridine Orange (+DNA) 502 526
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 514 518 543
Alexa Fluor® 532 534 553
ATTO 488 500 525
ATTO 495 495 527
ATTO 520 516 538
ATTO 532 501 545
BODIPY R6G 528 547
Calcein 494 514
Calcium Green-1 507 529
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
Dronpa 502 516
DY-505-Phalloidin 503 530
DyLight 488 493 518
Eosin 525 546
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Orange fluorescent microspheres 540 559
FluoSpheres Yellow-Green fluorescent microspheres 503 514
FM 1-43 473 579
Gold 525 521 551
Green 496 496 520
Green 500 501 524
HCS LipidTOX Green phospholipidosis 504 536
HiLyte Fluor™ 488 497 526
JO-JO-1, JO-PRO-1 530 544
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
mHoneyDew 478 562
mOrange2 551 565
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Oregon Green™ 488 498 526
Oregon Green™ 514 513 532
PicoGreen® 502 522
Qdot® 525 Nanocrystals 300 525
Qdot® 545 Nanocrystals 300 543
Rhodamine 110 497 519
Rhodamine 123 507 529
Rhodamine Green 497 523
Rhodol Green 497 524
Sodium Green 507 532
SpectrumGreen 497 538
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYTO 11 506 525
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
TO-PRO-1 515 531
TOTO-1 514 531
TurboYFP 525 538
Venus 516 528
VIC 530 550
Vybrant DyeCycle Green 506 534
Vybrant DyeCycle Orange 519 563
YFP (yellow GFP) 513 530
ZsYellow1 529 539

Specifications

LED-Venus-A-OMF

S-002998 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

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