Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.
This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring TRITC (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market. Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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2,566.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-554/23-25
FL-004612 / 0
- Tavg > 93% 543.0 – 566.0 nm
- Center Wavelength of 554.5 nm and GMBW of 23.0 nm
- FWHM of 28.1 nm
FF01-609/54-25
FL-004693 / 0
- Tavg > 93% 582 – 636 nm
- Center Wavelength of 609 nm and GMBW of 54 nm
- FWHM of 60.6 nm
FF573-Di01-25x36
FL-007123 / 0
- Dichroic Beamsplitter with edge at 573 nm
- Ravg > 98% 350 – 543 nm
- Tavg > 93% 580 – 636 nm
- Flatness / RWE: Standard Epi-fluorescence
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| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 32.0 mm | 5.0 mm |
| Emitter (Diameter) | 32.0 mm | 3.5 mm |
| Dichroic (L × W) | 32.0 mm × 44.0 mm (Corners must be cut for OFX cube) | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
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| Alexa Fluor® 546 | 556 | 572 |
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| Alexa Fluor® 555 | 553 | 565 |
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| Alexa Fluor® 568 | 579 | 603 |
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| Alexa Fluor® 594 | 590 | 618 |
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| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
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| AsRed 2 | 576 | 592 |
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| ATTO 550 | 553 | 576 |
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| ATTO 565 | 563 | 592 |
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| ATTO 590 | 594 | 624 |
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| BOBO™-3 | 570 | 605 |
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| BODIPY TMR-X | 544 | 573 |
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| BODIPY TR-X (MeOH) | 544 | 573 |
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| BODIPY TR-X phallacidin | 590 | 621 |
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| Calcium Crimson | 589 | 609 |
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| Calcium Orange | 589 | 609 |
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| CellTrace BODIPY TR methyl ester | 598 | 625 |
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| CellTracker Red CMTPX | 586 | 614 |
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| Cy3.5™ | 578 | 591 |
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| Dendra2 (Red) | 554 | 569 |
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| DiI | 551 | 569 |
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| DsRed | 559 | 583 |
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| DsRed-Express | 556 | 584 |
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| dTomato | 556 | 582 |
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| DY-590 | 582 | 599 |
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| DyLight 594 | 593 | 618 |
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| Ethidium bromide | 518 | 603 |
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| Ethidium homodimer | 527 | 617 |
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| FluoSpheres Red fluorescent microspheres | 577 | 607 |
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| HcRed1 | 588 | 618 |
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
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| KFP-Red | 575 | 599 |
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| LOLO-1 | 568 | 580 |
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| LysoTracker Red | 573 | 592 |
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| Magnesium Orange | 550 | 574 |
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| mApple | 566 | 594 |
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| mCherry | 587 | 610 |
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| Merocyanine 540 | 559 | 579 |
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| MitoTracker™ Orange | 551 | 575 |
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| MitoTracker™ Red | 578 | 598 |
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| mOrange | 546 | 562 |
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| mPlum | 589 | 649 |
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| mRFP | 555 | 583 |
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| mRFP1 | 584 | 700 |
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| mRuby | 558 | 605 |
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| mStrawberry | 574 | 596 |
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| mTangerine | 568 | 585 |
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| Nile red-phospolipid | 553 | 637 |
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| Nile red-triglyceride | 510 | 583 |
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| OFP | 547 | 567 |
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| pHrodo™, succinimidyl ester | 560 | 587 |
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| POPO-3 | 533 | 574 |
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| PO-PRO-3 | 539 | 570 |
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| Propidium Iodide (PI) | 305 | 617 |
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| Pro-Q Diamond | 556 | 583 |
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| Qdot® 585 Nanocrystals | 300 | 588 |
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| Qdot® 605 Nanocrystals | 300 | 602 |
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| Qdot® 625 Nanocrystals | 300 | 621 |
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| ReAsH-CCPGCC | 592 | 606 |
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| Red 580 | 580 | 603 |
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| Resorufin | 571 | 584 |
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| Rhod-2 | 553 | 577 |
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| Rhodamine phalloidin | 558 | 575 |
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| Rhodamine Red-X | 573 | 591 |
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| R-phycoerythrin (PE) | 565 | 576 |
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| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
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| SNARF-1 488nm (ph 6.0) | 548 | 586 |
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| SNARF-1 488nm (ph 9.0) | 576 | 638 |
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| SNARF-1 514nm (ph 6.0) | 549 | 587 |
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| SNARF-1 514nm (ph 9.0) | 576 | 638 |
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| SpectrumOrange | 554 | 587 |
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| Sulforhodamine 101-EtOH | 578 | 593 |
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| SYTOX Orange | 547 | 570 |
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| Tetramethylrhodamine dextran | 554 | 582 |
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| Texas Red DHPE | 584 | 608 |
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| TRITC (Tetramethylrhodamine) | 552 | 578 |
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| TurboFP635(Katushka) | 591 | 638 |
|
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| TurboRFP | 553 | 573 |
|
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| X-Rhod-1 Indicator | 580 | 601 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-554/23-25
FL-004612 / 0
- Tavg > 93% 543.0 – 566.0 nm
- Center Wavelength of 554.5 nm and GMBW of 23.0 nm
- FWHM of 28.1 nm
FF01-609/54-25
FL-004693 / 0
- Tavg > 93% 582 – 636 nm
- Center Wavelength of 609 nm and GMBW of 54 nm
- FWHM of 60.6 nm
FF573-Di01-25x36
FL-007123 / 0
- Dichroic Beamsplitter with edge at 573 nm
- Ravg > 98% 350 – 543 nm
- Tavg > 93% 580 – 636 nm
- Flatness / RWE: Standard Epi-fluorescence
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