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BrightLine Basic™ single-band filter set, optimized for TRITC and other like fluorophores

Semrock Part Number: LED-TRITC-A-Basic-000

IDEX Part Number: S-443183  /

Semrock

Our Brightline® Basic fluorescence filter set belongs to our essential performance group. These sets are suitable for various fluorescence applications ranging from detection to imaging. These filters feature a fundamental and basic design, offered at a competitive price point. They are designed with thin-film hard-coated technology, they ensure exceptional performance and reliability.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 550.45 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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  • Notify me when available
We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
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Set Components

FFB01-543/26-25

FL-442772

Each
  • Tavg > 93% 530 – 556 nm
  • Center Wavelength of 543 nm and GMBW of 26 nm
  • FWHM of 31.4 nm

FL-442772

FFB01-610/60-25

FL-442773

Each
  • Tavg > 93% 580 – 640 nm
  • Center Wavelength of 610 nm and GMBW of 60 nm
  • FWHM of 66.1 nm

FL-442773

FF574-Di01-25x36

FL-442641

Each
  • Dichroic Beamsplitter with edge at 574 nm
  • Ravg > 98% 350 – 566 nm
  • Tavg > 95% 582 – 1100 nm
  • Flatness / RWE: Standard Epi-fluorescence

FL-442641

Optical Specifications Value
Blocking BrightLine® Basic filters offer essential performance specifications. These sets are suitable for various fluorescence applications ranging from detection to imaging.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 80-50
Dichroic Orientation Reflective coating side should face toward light source.
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
7-AAD (7-aminoactinomycin D) 548 648
Alexa Fluor® 546 556 572
Alexa Fluor® 555 553 565
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
Amplex UltraRed peroxidation product (pH 7.5) 568 581
AsRed 2 576 592
ATTO 550 553 576
ATTO 565 563 592
ATTO 590 594 624
BOBO™-3 570 605
BODIPY TMR-X 544 573
BODIPY TR-X (MeOH) 544 573
BODIPY TR-X phallacidin 590 621
Calcium Crimson 589 609
Calcium Orange 589 609
CellTrace BODIPY TR methyl ester 598 625
CellTracker Red CMTPX 586 614
Cy3.5™ 578 591
Dendra2 (Red) 554 569
DiI 551 569
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-590 582 599
DyLight 594 593 618
Ethidium bromide 518 603
Ethidium homodimer 527 617
FluoSpheres Red fluorescent microspheres 577 607
HcRed1 588 618
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
KFP-Red 575 599
LOLO-1 568 580
LysoTracker Red 573 592
Magnesium Orange 550 574
mApple 566 594
mCherry 587 610
Merocyanine 540 559 579
MitoTracker™ Orange 551 575
MitoTracker™ Red 578 598
mOrange 546 562
mPlum 589 649
mRFP 555 583
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
Nile red-phospolipid 553 637
Nile red-triglyceride 510 583
OFP 547 567
pHrodo™, succinimidyl ester 560 587
POPO-3 533 574
PO-PRO-3 539 570
Propidium Iodide (PI) 305 617
Pro-Q Diamond 556 583
Qdot® 585 Nanocrystals 300 588
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
ReAsH-CCPGCC 592 606
Red 580 580 603
Resorufin 571 584
Rhod-2 553 577
Rhodamine phalloidin 558 575
Rhodamine Red-X 573 591
R-phycoerythrin (PE) 565 576
SNARF (carboxy) 514 Excitation pH9 576 638
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 488nm (ph 9.0) 576 638
SNARF-1 514nm (ph 6.0) 549 587
SNARF-1 514nm (ph 9.0) 576 638
SpectrumOrange 554 587
Sulforhodamine 101-EtOH 578 593
SYTOX Orange 547 570
Tetramethylrhodamine dextran 554 582
Texas Red DHPE 584 608
TRITC (Tetramethylrhodamine) 552 578
TurboFP635(Katushka) 591 638
TurboRFP 553 573
X-Rhod-1 Indicator 580 601

Specifications

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

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