BrightLine® single-band filter set, optimized for mTFP (Teal) and other like fluorophores, illuminated with LED-based light sources

Semrock Part Number: LED-mTFP-A-ZHE-ZERO

IDEX Part Number: S-001142  /

Semrock

This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring mTFP (Teal) (or other key fluorophores – see complete list above). This state-of-the-art set has been optimized for popular LED based light sources. This set has been designed to deliver significant fluorescence signal improvements for LED based light sources compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

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  • 1,463.00 USD Each
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FF01-442/42-25

FL-003929 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 421 – 462.5 nm
  • Center Wavelength of 441.5 nm and GMBW of 41.5 nm
  • FWHM of 46.2 nm

FF01-494/34-25

FL-004160 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 477 - 511 nm
  • Center Wavelength of 494 nm and GMBW of 34 nm
  • FWHM of 39.3 nm

FF470-Di01-25x36

FL-007031 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 470 nm
  • Ravg > 98% 350 - 462.5 nm
  • Tavg > 93% 477 - 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

1 of 1

This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help?
Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Acridine Yellow 470 550
AmCyan1 458 489
Aqua 431 430 478
ATTO 425 436 484
ATTO 465 453 507
BD Horizon V500 410 500
BOBO™-1 461 484
Cerulean 434 473
CFP (ECFP) 433 475
DY-415 418 470
ECFP 435 475
evoglow-Bs1 449 496
LysoSensor Green 448 502
mBBr+GSH 394 490
mTFP1 462 492
POPO-1 433 457
PO-PRO-1 433 456
SpectrumAqua 434 481
SYTO 45 451 485
SYTOX Blue 444 470
TagCFP 458 480

Related Products

FF01-442/42-25

FL-003929 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 421 – 462.5 nm
  • Center Wavelength of 441.5 nm and GMBW of 41.5 nm
  • FWHM of 46.2 nm

FF01-494/34-25

FL-004160 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 477 - 511 nm
  • Center Wavelength of 494 nm and GMBW of 34 nm
  • FWHM of 39.3 nm

FF470-Di01-25x36

FL-007031 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 470 nm
  • Ravg > 98% 350 - 462.5 nm
  • Tavg > 93% 477 - 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

1 of 1

This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help?
Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.