Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.
This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring mCherry (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market. Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
-
EachUS Domestic pricing – contact your local distributor for other currency list prices
-
1,648.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
-
-
0.000.00 -
-
- Notify me when available
total selected
Clear selection
Select all 3 products from all pages.
FF02-641/75-25
FL-004436 / 0
- Tavg > 93% 604 – 679 nm
- Center Wavelength of 641.5 nm and GMBW of 75 nm
- FWHM of 81.7 nm
FF01-578/21-25
FL-004616 / 0
- Tavg > 93% 567.4 – 588.6 nm
- Center Wavelength of 578 nm and GMBW of 21.2 nm
- FWHM of 26.1 nm
FF596-Di01-25x36
FL-007156 / 0
- Dichroic Beamsplitter with edge at 596 nm
- Ravg > 98% 350 – 588.6 nm
- Tavg > 93% 603 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
|
|
| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
|
|
| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
|
| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
|
| Alexa Fluor® 568 | 579 | 603 |
|
|
| Alexa Fluor® 594 | 590 | 618 |
|
|
| APC (Allophycocyanin) | 651 | 660 |
|
|
| AsRed 2 | 576 | 592 |
|
|
| ATTO 565 | 563 | 592 |
|
|
| ATTO 590 | 594 | 624 |
|
|
| ATTO 594 | 601 | 624 |
|
|
| ATTO 610 | 614 | 634 |
|
|
| BOBO™-3 | 570 | 605 |
|
|
| BODIPY TR-X phallacidin | 590 | 621 |
|
|
| Calcium Crimson | 589 | 609 |
|
|
| Carboxynaphthofluorescein | 599 | 674 |
|
|
| CellTrace BODIPY TR methyl ester | 598 | 625 |
|
|
| CellTracker Red CMTPX | 586 | 614 |
|
|
| Cy3.5™ | 578 | 591 |
|
|
| DDAO | 648 | 657 |
|
|
| DY-590 | 582 | 599 |
|
|
| DY-630 | 634 | 656 |
|
|
| DyLight 594 | 593 | 618 |
|
|
| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
|
|
| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
|
| HcRed1 | 588 | 618 |
|
|
| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
|
|
| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
|
| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
|
| HiLyte Fluor™ 594 | 592 | 616 |
|
|
| KFP-Red | 575 | 599 |
|
|
| LIVE-DEAD Fixable Red Dead Cell Stain | 595 | 613 |
|
|
| LysoTracker Red | 573 | 592 |
|
|
| mApple | 566 | 594 |
|
|
| mCherry | 587 | 610 |
|
|
| MitoTracker™ Red | 578 | 598 |
|
|
| mPlum | 589 | 649 |
|
|
| mRFP1 | 584 | 700 |
|
|
| mRuby | 558 | 605 |
|
|
| mStrawberry | 574 | 596 |
|
|
| mTangerine | 568 | 585 |
|
|
| Nile blue-EtOH | 631 | 660 |
|
|
| Nile red-phospolipid | 553 | 637 |
|
|
| PerCP | 490 | 677 |
|
|
| Propidium Iodide (PI) | 305 | 617 |
|
|
| Qdot® 605 Nanocrystals | 300 | 602 |
|
|
| Qdot® 625 Nanocrystals | 300 | 621 |
|
|
| Qdot® 655 Nanocrystals | 300 | 654 |
|
|
| ReAsH-CCPGCC | 592 | 606 |
|
|
| Red 580 | 580 | 603 |
|
|
| Rhodamine Red-X | 573 | 591 |
|
|
| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
|
| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
|
| SNARF-1 514nm (ph 9.0) | 576 | 638 |
|
|
| SpectrumRed | 587 | 615 |
|
|
| Sulforhodamine 101-EtOH | 578 | 593 |
|
|
| SYTO 17 | 619 | 638 |
|
|
| SYTO 61 | 618 | 650 |
|
|
| Texas Red dextran | 592 | 613 |
|
|
| Texas Red DHPE | 584 | 608 |
|
|
| Texas Red-X® | 595 | 613 |
|
|
| TurboFP635(Katushka) | 591 | 638 |
|
|
| X-Rhod-1 Indicator | 580 | 601 |
|
|
| YO-PRO-3 | 613 | 629 |
|
|
| YOYO-3 | 611 | 631 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF02-641/75-25
FL-004436 / 0
- Tavg > 93% 604 – 679 nm
- Center Wavelength of 641.5 nm and GMBW of 75 nm
- FWHM of 81.7 nm
FF01-578/21-25
FL-004616 / 0
- Tavg > 93% 567.4 – 588.6 nm
- Center Wavelength of 578 nm and GMBW of 21.2 nm
- FWHM of 26.1 nm
FF596-Di01-25x36
FL-007156 / 0
- Dichroic Beamsplitter with edge at 596 nm
- Ravg > 98% 350 – 588.6 nm
- Tavg > 93% 603 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
You might also be interested in the following products
Please contact your Partner with ARV Optics to purchase.
To purchase this product, click into product details for more information.
Adding...
Removing
Processing...
{{productContent.processedProductsCount}} of {{productContent.model.matrixProducts.length}}