Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.
This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring mCherry (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market. Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,648.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF02-641/75-25
FL-004436 / 0
- Tavg > 93% 604 – 679 nm
- Center Wavelength of 641.5 nm and GMBW of 75 nm
- FWHM of 81.7 nm
FF01-578/21-25
FL-004616 / 0
- Tavg > 93% 567.4 – 588.6 nm
- Center Wavelength of 578 nm and GMBW of 21.2 nm
- FWHM of 26.1 nm
FF596-Di01-25x36
FL-007156 / 0
- Dichroic Beamsplitter with edge at 596 nm
- Ravg > 98% 350 – 588.6 nm
- Tavg > 93% 603 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
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This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
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| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
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| Alexa Fluor® 568 | 579 | 603 |
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| Alexa Fluor® 594 | 590 | 618 |
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| APC (Allophycocyanin) | 651 | 660 |
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| AsRed 2 | 576 | 592 |
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| ATTO 565 | 563 | 592 |
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| ATTO 590 | 594 | 624 |
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| ATTO 594 | 601 | 624 |
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| ATTO 610 | 614 | 634 |
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| BOBO™-3 | 570 | 605 |
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| BODIPY TR-X phallacidin | 590 | 621 |
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| Calcium Crimson | 589 | 609 |
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| Carboxynaphthofluorescein | 599 | 674 |
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| CellTrace BODIPY TR methyl ester | 598 | 625 |
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| CellTracker Red CMTPX | 586 | 614 |
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| Cy3.5™ | 578 | 591 |
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| DDAO | 648 | 657 |
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| DY-590 | 582 | 599 |
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| DY-630 | 634 | 656 |
|
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| DyLight 594 | 593 | 618 |
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| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
|
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| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
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| HcRed1 | 588 | 618 |
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| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
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| HiLyte Fluor™ 594 | 592 | 616 |
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| KFP-Red | 575 | 599 |
|
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| LIVE-DEAD Fixable Red Dead Cell Stain | 595 | 613 |
|
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| LysoTracker Red | 573 | 592 |
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| mApple | 566 | 594 |
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| mCherry | 587 | 610 |
|
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| MitoTracker™ Red | 578 | 598 |
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| mPlum | 589 | 649 |
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| mRFP1 | 584 | 700 |
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| mRuby | 558 | 605 |
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| mStrawberry | 574 | 596 |
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| mTangerine | 568 | 585 |
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| Nile blue-EtOH | 631 | 660 |
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| Nile red-phospolipid | 553 | 637 |
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| PerCP | 490 | 677 |
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| Propidium Iodide (PI) | 305 | 617 |
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| Qdot® 605 Nanocrystals | 300 | 602 |
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| Qdot® 625 Nanocrystals | 300 | 621 |
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| Qdot® 655 Nanocrystals | 300 | 654 |
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| ReAsH-CCPGCC | 592 | 606 |
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| Red 580 | 580 | 603 |
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| Rhodamine Red-X | 573 | 591 |
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| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
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| SNARF-1 488nm (ph 9.0) | 576 | 638 |
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| SNARF-1 514nm (ph 9.0) | 576 | 638 |
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| SpectrumRed | 587 | 615 |
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| Sulforhodamine 101-EtOH | 578 | 593 |
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| SYTO 17 | 619 | 638 |
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| SYTO 61 | 618 | 650 |
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| Texas Red dextran | 592 | 613 |
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| Texas Red DHPE | 584 | 608 |
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| Texas Red-X® | 595 | 613 |
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| TurboFP635(Katushka) | 591 | 638 |
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| X-Rhod-1 Indicator | 580 | 601 |
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| YO-PRO-3 | 613 | 629 |
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| YOYO-3 | 611 | 631 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF02-641/75-25
FL-004436 / 0
- Tavg > 93% 604 – 679 nm
- Center Wavelength of 641.5 nm and GMBW of 75 nm
- FWHM of 81.7 nm
FF01-578/21-25
FL-004616 / 0
- Tavg > 93% 567.4 – 588.6 nm
- Center Wavelength of 578 nm and GMBW of 21.2 nm
- FWHM of 26.1 nm
FF596-Di01-25x36
FL-007156 / 0
- Dichroic Beamsplitter with edge at 596 nm
- Ravg > 98% 350 – 588.6 nm
- Tavg > 93% 603 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
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