BrightLine® single-band filter set, optimized for mCherry and other like fluorophores, illuminated with LED-based light sources

Semrock Part Number: LED-mCherry-A-LDMK-ZERO

IDEX Part Number: S-001699  /

Semrock

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring mCherry (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market. Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

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  • 1,628.00 USD Each
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FF02-641/75-25

FL-004436 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 604 – 679 nm
  • Center Wavelength of 641.5 nm and GMBW of 75 nm
  • FWHM of 81.7 nm

FF01-578/21-25

FL-004616 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 567.4 – 588.6 nm
  • Center Wavelength of 578 nm and GMBW of 21.2 nm
  • FWHM of 26.1 nm

FF596-Di01-25x36

FL-007156 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 596 nm
  • Ravg > 98% 350 – 588.6 nm
  • Tavg > 93% 603 – 950 nm
  •  Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)21.8 mm5.0 mm
Emitter (Diameter)23.3 mm5.0 mm
Dichroic (L × W)22.0 mm × 29.0 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help?
Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-carboxynapthofluorescein (pH 10) 555 615
5-ROX (carboxy-X-rhodamine) 578 604
7-AAD (7-aminoactinomycin D) 548 648
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
APC (Allophycocyanin) 651 660
AsRed 2 576 592
ATTO 565 563 592
ATTO 590 594 624
ATTO 594 601 624
ATTO 610 614 634
BOBO™-3 570 605
BODIPY TR-X phallacidin 590 621
Calcium Crimson 589 609
Carboxynaphthofluorescein 599 674
CellTrace BODIPY TR methyl ester 598 625
CellTracker Red CMTPX 586 614
Cy3.5™ 578 591
DDAO 648 657
DY-590 582 599
DY-630 634 656
DyLight 594 593 618
FluoSpheres Crimson fluorescent microspheres 620 646
FluoSpheres Red fluorescent microspheres 577 607
HcRed1 588 618
HCS LipidTOX Deep Red neutral lipid stain 635 652
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 594 592 616
KFP-Red 575 599
LIVE-DEAD Fixable Red Dead Cell Stain 595 613
LysoTracker Red 573 592
mApple 566 594
mCherry 587 610
MitoTracker™ Red 578 598
mPlum 589 649
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
Nile blue-EtOH 631 660
Nile red-phospolipid 553 637
PerCP 490 677
Propidium Iodide (PI) 305 617
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
Qdot® 655 Nanocrystals 300 654
ReAsH-CCPGCC 592 606
Red 580 580 603
Rhodamine Red-X 573 591
SNARF (carboxy) 514 Excitation pH9 576 638
SNARF-1 488nm (ph 9.0) 576 638
SNARF-1 514nm (ph 9.0) 576 638
SpectrumRed 587 615
Sulforhodamine 101-EtOH 578 593
SYTO 17 619 638
SYTO 61 618 650
Texas Red dextran 592 613
Texas Red DHPE 584 608
Texas Red-X® 595 613
TurboFP635(Katushka) 591 638
X-Rhod-1 Indicator 580 601
YO-PRO-3 613 629
YOYO-3 611 631

Related Products

FF02-641/75-25

FL-004436 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 604 – 679 nm
  • Center Wavelength of 641.5 nm and GMBW of 75 nm
  • FWHM of 81.7 nm

FF01-578/21-25

FL-004616 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 567.4 – 588.6 nm
  • Center Wavelength of 578 nm and GMBW of 21.2 nm
  • FWHM of 26.1 nm

FF596-Di01-25x36

FL-007156 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 596 nm
  • Ravg > 98% 350 – 588.6 nm
  • Tavg > 93% 603 – 950 nm
  •  Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)21.8 mm5.0 mm
Emitter (Diameter)23.3 mm5.0 mm
Dichroic (L × W)22.0 mm × 29.0 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help?
Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.