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BrightLine® 2.0 single-band filter set, optimized for FITC and other like fluorophores, illuminated with LED-based light engines

Semrock Part Number: LED-FITC-B-000

IDEX Part Number: S-443312  /

Semrock

BrightLine™ 2.0 filter sets introduces a unified, improved design that delivers enhanced contrast and leverages the latest advances in coating technologies across this industry-leading filter family. The fluorescence bandpass filters and sets are meticulously optimized for seamless integration across a wide range of fluorescence instruments. With its all-thin-film, hard-coated construction, BrightLine 2.0 sets a new standard in performance and reliability.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 908.43 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Filter set mounted in microscope holders/cubes found below in related products section.
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Set Components

FF01-475/27-25

FL-443308

Each
  • Tavg > 95% 461 – 488 nm
  • Center Wavelength of 474.5 nm and GMBW of 27 nm
  • FWHM of 31.7 nm

FL-443308

FF01-525/46-25

FL-443311

Each
  • Tavg > 95% 502 – 548 nm
  • Center Wavelength of 525 nm and GMBW of 46 nm
  • FWHM of 51.4 nm

FL-443311

FF495-Di04-25x36

FL-439557

Each
  • Dichroic Beamsplitter with edge at 495 nm
  • Ravg > 98% 350 – 488 nm
  • Tavg > 95% 502 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

FL-439557

Optical Specifications Value
Blocking BrightLine® 2.0 filters have blocking far exceeding OD 8 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Exciter/Emitter Orientation Arrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation Reflective surface marked with part number - Orient in direction of incoming light
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
LinkDescription
Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
Access Optical Filter WarrantyFind our warranty in our Terms & Conditions of Sale.
Semrock Optical Filters RoHS CertificateRoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances.
Semrock Optical Filters REACH CertificateREACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry.
Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-FAM (5-carboxyfluorescein) 492 518
Acridine Orange (+DNA) 502 526
Acridine Yellow 470 550
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
AmCyan1 458 489
ATTO 465 453 507
ATTO 488 500 525
ATTO 495 495 527
BOBO™-1 461 484
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
Calcein 494 514
CFP2 490 510
Cy2™ 492 507
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
Dendra2 (Green) 491 507
DiA 457 586
DiO 475 500
Dronpa 502 516
DY-505-Phalloidin 503 530
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
evoglow-Bs1 449 496
FITC (Fluorescein) 495 519
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Yellow-Green fluorescent microspheres 503 514
FM 1-43 473 579
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HCS LipidTOX Green phospholipidosis 504 536
HiLyte Fluor™ 488 497 526
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LysoSensor Green 448 502
LysoTracker Green 501 509
LysoTracker Yellow HCK-123 488 565
mHoneyDew 478 562
MitoTracker™ Green 490 512
mTFP1 462 492
mWasabi 493 509
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Oregon Green™ 488 498 526
PicoGreen® 502 522
PKH67 488 500
Qdot® 525 Nanocrystals 300 525
Qdot® 545 Nanocrystals 300 543
Rhodamine 110 497 519
Rhodamine Green 497 523
Rhodol Green 497 524
sgGFP™ (super glow GFP) 472 506
Sodium Green 507 532
SpectrumGreen 497 538
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYTO 11 506 525
SYTO 13 448 506
SYTO 16 489 520
SYTO 9 483 500
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
TagCFP 458 480
TOTO-1 514 531
TurboGFP 482 503
Vybrant DyeCycle Green 506 534
wtGFP (wild type GFP, non-UV excitation) 474 509
YO-PRO-1 491 506
YOYO-1 491 508

Specifications

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
LinkDescription
Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
Access Optical Filter WarrantyFind our warranty in our Terms & Conditions of Sale.
Semrock Optical Filters RoHS CertificateRoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances.
Semrock Optical Filters REACH CertificateREACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry.
Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.

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