Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.
This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring FITC (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market. Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,623.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-474/27-25
FL-004596 / 0
- Tavg > 93% 461.0 – 487.5 nm
- Center Wavelength of 474.3 nm and GMBW of 26.5 nm
- FWHM of 30.9 nm
FF01-525/45-25
FL-004680 / 0
- Tavg > 93% 502.5 – 547.5 nm
- Center Wavelength of 525 nm and GMBW of 45 nm
- FWHM of 50.6 nm
FF495-Di03-25x36
FL-007145 / 0
- Dichroic Beamsplitter with edge at 495 nm
- Ravg > 98% 350 – 487.5 nm
- Tavg > 93% 502.5 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
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This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.5 mm |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Acridine Yellow | 470 | 550 |
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| Alexa Fluor® 430 | 431 | 541 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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| AmCyan1 | 458 | 489 |
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| ATTO 465 | 453 | 507 |
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| ATTO 488 | 500 | 525 |
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| ATTO 495 | 495 | 527 |
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| BOBO™-1 | 461 | 484 |
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| BODIPY FL | 505 | 512 |
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| BODIPY FL-MeOH | 502 | 511 |
|
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| Calcein | 494 | 514 |
|
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| CFP2 | 490 | 510 |
|
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| Cy2™ | 492 | 507 |
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| CyQUANT GR-DNA | 502 | 523 |
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| DAF-FM-NO | 495 | 519 |
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| Dendra2 (Green) | 491 | 507 |
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| DiA | 457 | 586 |
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| DiO | 475 | 500 |
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| Dronpa | 502 | 516 |
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| DY-505-Phalloidin | 503 | 530 |
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| ecliptic pHluorin pH5.5 | 473 | 507 |
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| Emerald | 491 | 511 |
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| evoglow-Bs1 | 449 | 496 |
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| FITC (Fluorescein) | 495 | 519 |
|
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| Fluo-4 | 494 | 516 |
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| Fluorescein dextran | 501 | 524 |
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| Fluorescein-pH 8.0 | 489 | 517 |
|
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| Fluoro-Emerald | 494 | 518 |
|
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
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| FM 1-43 | 473 | 579 |
|
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| GFP (EGFP) | 489 | 511 |
|
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| Green 496 | 496 | 520 |
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| Green 500 | 501 | 524 |
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
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| HiLyte Fluor™ 488 | 497 | 526 |
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
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| LysoSensor Green | 448 | 502 |
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| LysoTracker Green | 501 | 509 |
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| LysoTracker Yellow HCK-123 | 488 | 565 |
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| mHoneyDew | 478 | 562 |
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| MitoTracker™ Green | 490 | 512 |
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| mTFP1 | 462 | 492 |
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| mWasabi | 493 | 509 |
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| NBD-X (MeOH) | 467 | 538 |
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
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| Oregon Green™ 488 | 498 | 526 |
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| PicoGreen® | 502 | 522 |
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| PKH67 | 488 | 500 |
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| Qdot® 525 Nanocrystals | 300 | 525 |
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| Qdot® 545 Nanocrystals | 300 | 543 |
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| Rhodamine 110 | 497 | 519 |
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| Rhodamine Green | 497 | 523 |
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| Rhodol Green | 497 | 524 |
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| sgGFP™ (super glow GFP) | 472 | 506 |
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| Sodium Green | 507 | 532 |
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| SpectrumGreen | 497 | 538 |
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
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| SYTO 11 | 506 | 525 |
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| SYTO 13 | 448 | 506 |
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| SYTO 16 | 489 | 520 |
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| SYTO 9 | 483 | 500 |
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
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| SYTOX Green-DNA | 504 | 524 |
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| TagCFP | 458 | 480 |
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| TOTO-1 | 514 | 531 |
|
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| TurboGFP | 482 | 503 |
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| Vybrant DyeCycle Green | 506 | 534 |
|
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| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
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| YO-PRO-1 | 491 | 506 |
|
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| YOYO-1 | 491 | 508 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-474/27-25
FL-004596 / 0
- Tavg > 93% 461.0 – 487.5 nm
- Center Wavelength of 474.3 nm and GMBW of 26.5 nm
- FWHM of 30.9 nm
FF01-525/45-25
FL-004680 / 0
- Tavg > 93% 502.5 – 547.5 nm
- Center Wavelength of 525 nm and GMBW of 45 nm
- FWHM of 50.6 nm
FF495-Di03-25x36
FL-007145 / 0
- Dichroic Beamsplitter with edge at 495 nm
- Ravg > 98% 350 – 487.5 nm
- Tavg > 93% 502.5 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.5 mm |
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