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BrightLine® single-band filter set, optimized for DAPI and other like fluorophores, illuminated with 365 to 395 nm LED-based light engines

Semrock Part Number: LED-DAPI-B-NQF

IDEX Part Number: S-001145  /

Semrock
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
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    US Domestic pricing – contact your local distributor for other currency list prices
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  • 1,464.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm
The Nikon “true” cube needs a Di with corner chamfered (-QF)		1.1 mm
(Can accept a 2mm dichroic)
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
Alexa Fluor® 405 401 422
Aqua 431 430 478
ATTO 390 392 477
BD Horizon V450 406 449
BFP (EBFP) 380 440
Cascade Blue™ 401 419
CellTrace calcein violet 400 452
CFP (ECFP) 433 475
DAPI 359 461
DY-415 418 470
DyLight 405 398 420
ECFP 435 475
HiLyte Fluor™ 405 403 428
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
LysoSensor Blue 374 424
Marina Blue 363 461
mBBr+GSH 394 490
Pacific Blue™ 404 455
sgBFP™ 387 451
SpectrumBlue 405 449
TagBFP 402 457
Vybrant DyeCycle Violet 369 437

NQF Holder/Cube

This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm
The Nikon “true” cube needs a Di with corner chamfered (-QF)		1.1 mm
(Can accept a 2mm dichroic)

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