Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.
This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring DAPI (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market. Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,464.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-378/52-25
FL-004583 / 0
- Tavg > 85% 352 – 404 nm
- Center Wavelength of 378 nm and GMBW of 52 nm
- FWHM of 54.6 nm
FF02-447/60-25
FL-004651 / 0
- Tavg > 93% 417 – 477 nm
- Center Wavelength of 447 nm and GMBW of 60 nm
- FWHM of 65.3 nm
FF409-Di03-25x36
FL-007109 / 0
- Dichroic Beamsplitter with edge at 409 nm
- Ravg > 98% 327 – 404 nm
- Tavg > 93% 415 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
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| Alexa Fluor® 405 | 401 | 422 |
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| Aqua 431 | 430 | 478 |
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| ATTO 390 | 392 | 477 |
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| BD Horizon V450 | 406 | 449 |
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| BFP (EBFP) | 380 | 440 |
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| Cascade Blue™ | 401 | 419 |
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| CellTrace calcein violet | 400 | 452 |
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| CFP (ECFP) | 433 | 475 |
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| DAPI | 359 | 461 |
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| DY-415 | 418 | 470 |
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| DyLight 405 | 398 | 420 |
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| ECFP | 435 | 475 |
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| HiLyte Fluor™ 405 | 403 | 428 |
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| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
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| LysoSensor Blue | 374 | 424 |
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| Marina Blue | 363 | 461 |
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| mBBr+GSH | 394 | 490 |
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| Pacific Blue™ | 404 | 455 |
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| sgBFP™ | 387 | 451 |
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| SpectrumBlue | 405 | 449 |
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| TagBFP | 402 | 457 |
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| Vybrant DyeCycle Violet | 369 | 437 |
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total selected
Clear selection
Select all 3 products from all pages.
FF01-378/52-25
FL-004583 / 0
- Tavg > 85% 352 – 404 nm
- Center Wavelength of 378 nm and GMBW of 52 nm
- FWHM of 54.6 nm
FF02-447/60-25
FL-004651 / 0
- Tavg > 93% 417 – 477 nm
- Center Wavelength of 447 nm and GMBW of 60 nm
- FWHM of 65.3 nm
FF409-Di03-25x36
FL-007109 / 0
- Dichroic Beamsplitter with edge at 409 nm
- Ravg > 98% 327 – 404 nm
- Tavg > 93% 415 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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