Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.
This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring DAPI (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market. Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,164.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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0.000.00 -
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- Notify me when available
Filter set mounted in microscope holders/cubes found below in related products section.
Set Components
FF01-378/52-25FL-004583
Each
|
FL-004583 |
|
FF02-447/60-25-ZFL-004572
Each
|
FL-004572 |
|
FF409-Di03-25x36-ZFL-007085
Each
Custom sizing delivery confirmed ARO (sizing fee applies). |
FL-007085 |
total selected
Clear selection
Select all 3 products from all pages.
FF01-378/52-25
FL-004583 / 0
- Tavg > 85% 352 – 404 nm
- Center Wavelength of 378 nm and GMBW of 52 nm
- FWHM of 54.6 nm
FF02-447/60-25
FL-004651 / 0
- Tavg > 93% 417 – 477 nm
- Center Wavelength of 447 nm and GMBW of 60 nm
- FWHM of 65.3 nm
FF409-Di03-25x36
FL-007109 / 0
- Dichroic Beamsplitter with edge at 409 nm
- Ravg > 98% 327 – 404 nm
- Tavg > 93% 415 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
| Topic | Description | |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters | |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters | |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD | |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? |
|
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
|
|
| Alexa Fluor® 405 | 401 | 422 |
|
|
| Aqua 431 | 430 | 478 |
|
|
| ATTO 390 | 392 | 477 |
|
|
| BD Horizon V450 | 406 | 449 |
|
|
| BFP (EBFP) | 380 | 440 |
|
|
| Cascade Blue™ | 401 | 419 |
|
|
| CellTrace calcein violet | 400 | 452 |
|
|
| CFP (ECFP) | 433 | 475 |
|
|
| DAPI | 359 | 461 |
|
|
| DY-415 | 418 | 470 |
|
|
| DyLight 405 | 398 | 420 |
|
|
| ECFP | 435 | 475 |
|
|
| HiLyte Fluor™ 405 | 403 | 428 |
|
|
| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
|
|
| LysoSensor Blue | 374 | 424 |
|
|
| Marina Blue | 363 | 461 |
|
|
| mBBr+GSH | 394 | 490 |
|
|
| Pacific Blue™ | 404 | 455 |
|
|
| sgBFP™ | 387 | 451 |
|
|
| SpectrumBlue | 405 | 449 |
|
|
| TagBFP | 402 | 457 |
|
|
| Vybrant DyeCycle Violet | 369 | 437 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-378/52-25
FL-004583 / 0
- Tavg > 85% 352 – 404 nm
- Center Wavelength of 378 nm and GMBW of 52 nm
- FWHM of 54.6 nm
FF02-447/60-25
FL-004651 / 0
- Tavg > 93% 417 – 477 nm
- Center Wavelength of 447 nm and GMBW of 60 nm
- FWHM of 65.3 nm
FF409-Di03-25x36
FL-007109 / 0
- Dichroic Beamsplitter with edge at 409 nm
- Ravg > 98% 327 – 404 nm
- Tavg > 93% 415 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
| Topic | Description | |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters | |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters | |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD | |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? |
|
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