This Full-multiband triple-band set is designed for imaging DAPI, FITC, and Texas Red (and other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in triple-band performance, and has been optimized for popular LED-based light engines, including 365 nm DAPI LEDs. This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter, emitter, and exciter filter, all with "no burn-out" performance.
Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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2,139.62 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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Filter set mounted in microscope holders/cubes found below in related products section.
Set Components
FF01-378/474/575-25FL-004582
Each
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FL-004582 |
|
|
|
FL-003866 |
|
|
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FL-006719 |
|
TE 2000 Fluorescence Filter Holder for Nikon MicroscopesNTE
Each
Compatible microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
|
NTE |
total selected
Clear selection
Select all 3 products from all pages.
FF01-432/523/702-25
FL-004189 / 0
- Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 546 nm & Tavg > 93% 604 – 800 nm
- Center Wavelengths of 432 nm, 522.8 nm & 702 nm and GMBWs of 36 nm, 46.5 nm & 196 nm
FF01-378/474/575-25
FL-004582 / 0
- Tavg > 85% 352 – 404 nm, Tavg > 93% 461 – 487.5 nm & Tavg > 93% 562.5 – 587.5 nm
- Center Wavelengths of 378 nm, 474 nm & 575 nm and GMBWs of 52 nm, 26.5 nm & 25 nm
FF409/493/596-Di02-25x36
FL-007086 / 0
- Dichroic Beamsplitter with edges at 409 nm, 493 nm & 596 nm
- Ravg > 95% 350 – 404 nm, Ravg > 95% 461 – 487.5 nm & Ravg > 95% 559.5 – 589.5 nm
- Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 546 nm & Tavg > 93% 604 – 850 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
|
|
| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
|
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
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| Acridine Orange (+DNA) | 502 | 526 |
|
|
| Acridine Yellow | 470 | 550 |
|
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| Alexa Fluor® 405 | 401 | 422 |
|
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| Alexa Fluor® 430 | 431 | 541 |
|
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| Alexa Fluor® 488 | 499 | 520 |
|
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| Alexa Fluor® 500 | 503 | 525 |
|
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| Alexa Fluor® 568 | 579 | 603 |
|
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| Alexa Fluor® 594 | 590 | 618 |
|
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| Alexa Fluor® 660 | 663 | 691 |
|
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| AmCyan1 | 458 | 489 |
|
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| AsRed 2 | 576 | 592 |
|
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| ATTO 465 | 453 | 507 |
|
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| ATTO 488 | 500 | 525 |
|
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| ATTO 495 | 495 | 527 |
|
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| ATTO 565 | 563 | 592 |
|
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| ATTO 590 | 594 | 624 |
|
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| ATTO 594 | 601 | 624 |
|
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| BD Horizon V450 | 406 | 449 |
|
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| BFP (EBFP) | 380 | 440 |
|
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| BOBO™-1 | 461 | 484 |
|
|
| BOBO™-3 | 570 | 605 |
|
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| BODIPY FL | 505 | 512 |
|
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| BODIPY FL-MeOH | 502 | 511 |
|
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| BODIPY TR-X phallacidin | 590 | 621 |
|
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| Calcein | 494 | 514 |
|
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| Calcium Crimson | 589 | 609 |
|
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| Carboxynaphthofluorescein | 599 | 674 |
|
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| Cascade Blue™ | 401 | 419 |
|
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| CellTrace BODIPY TR methyl ester | 598 | 625 |
|
|
| CellTrace calcein violet | 400 | 452 |
|
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| CellTracker Red CMTPX | 586 | 614 |
|
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| CFP2 | 490 | 510 |
|
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| Cy2™ | 492 | 507 |
|
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| Cy3.5™ | 578 | 591 |
|
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| CyQUANT GR-DNA | 502 | 523 |
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| DAF-FM-NO | 495 | 519 |
|
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| DAPI | 359 | 461 |
|
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| DDAO | 648 | 657 |
|
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| Dendra2 (Green) | 491 | 507 |
|
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| DiO | 475 | 500 |
|
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| Dronpa | 502 | 516 |
|
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| DY-505-Phalloidin | 503 | 530 |
|
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| DY-590 | 582 | 599 |
|
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| DY-630 | 634 | 656 |
|
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| DyLight 405 | 398 | 420 |
|
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| DyLight 594 | 593 | 618 |
|
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| ecliptic pHluorin pH5.5 | 473 | 507 |
|
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| Emerald | 491 | 511 |
|
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| evoglow-Bs1 | 449 | 496 |
|
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| FITC (Fluorescein) | 495 | 519 |
|
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| Fluo-4 | 494 | 516 |
|
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| Fluorescein dextran | 501 | 524 |
|
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| Fluorescein-pH 8.0 | 489 | 517 |
|
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| Fluoro-Emerald | 494 | 518 |
|
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| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
|
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| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
|
| FM 4-64 | 505 | 725 |
|
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| GFP (EGFP) | 489 | 511 |
|
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| Green 496 | 496 | 520 |
|
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| Green 500 | 501 | 524 |
|
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| HcRed1 | 588 | 618 |
|
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
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| HiLyte Fluor™ 405 | 403 | 428 |
|
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| HiLyte Fluor™ 488 | 497 | 526 |
|
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| HiLyte Fluor™ 594 | 592 | 616 |
|
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| KFP-Red | 575 | 599 |
|
|
| LDS 751 | 560 | 711 |
|
|
| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
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| LIVE-DEAD Fixable Red Dead Cell Stain | 595 | 613 |
|
|
| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
|
|
| LysoSensor Blue | 374 | 424 |
|
|
| LysoSensor Green | 448 | 502 |
|
|
| LysoTracker Green | 501 | 509 |
|
|
| LysoTracker Red | 573 | 592 |
|
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| LysoTracker Yellow HCK-123 | 488 | 565 |
|
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| mApple | 566 | 594 |
|
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| Marina Blue | 363 | 461 |
|
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| mCherry | 587 | 610 |
|
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| mHoneyDew | 478 | 562 |
|
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| MitoTracker™ Green | 490 | 512 |
|
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| MitoTracker™ Red | 578 | 598 |
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| mPlum | 589 | 649 |
|
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| mRFP1 | 584 | 700 |
|
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| mRuby | 558 | 605 |
|
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| mStrawberry | 574 | 596 |
|
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| mTFP1 | 462 | 492 |
|
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| mWasabi | 493 | 509 |
|
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| NBD-X (MeOH) | 467 | 538 |
|
|
| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
|
| Nile blue-EtOH | 631 | 660 |
|
|
| Nile red-phospolipid | 553 | 637 |
|
|
| Orange 552 | 552 | 574 |
|
|
| Oregon Green™ 488 | 498 | 526 |
|
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| Pacific Blue™ | 404 | 455 |
|
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| PerCP | 490 | 677 |
|
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| PicoGreen® | 502 | 522 |
|
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| PKH67 | 488 | 500 |
|
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| Propidium Iodide (PI) | 305 | 617 |
|
|
| Qdot® 525 Nanocrystals | 300 | 525 |
|
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| Qdot® 545 Nanocrystals | 300 | 543 |
|
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| Qdot® 605 Nanocrystals | 300 | 602 |
|
|
| Qdot® 625 Nanocrystals | 300 | 621 |
|
|
| Qdot® 655 Nanocrystals | 300 | 654 |
|
|
| Qdot® 705 Nanocrystals | 300 | 702 |
|
|
| Qdot® 800 Nanocrystals | 300 | 792 |
|
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| ReAsH-CCPGCC | 592 | 606 |
|
|
| Red 580 | 580 | 603 |
|
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| Rhodamine 110 | 497 | 519 |
|
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| Rhodamine Green | 497 | 523 |
|
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| Rhodamine Red-X | 573 | 591 |
|
|
| Rhodol Green | 497 | 524 |
|
|
| sgBFP™ | 387 | 451 |
|
|
| sgGFP™ (super glow GFP) | 472 | 506 |
|
|
| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
|
| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
|
| SNARF-1 514nm (ph 9.0) | 576 | 638 |
|
|
| Sodium Green | 507 | 532 |
|
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| SpectrumBlue | 405 | 449 |
|
|
| SpectrumGreen | 497 | 538 |
|
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| SpectrumRed | 587 | 615 |
|
|
| Sulforhodamine 101-EtOH | 578 | 593 |
|
|
| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
|
| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
|
| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
|
| SYTO 11 | 506 | 525 |
|
|
| SYTO 13 | 448 | 506 |
|
|
| SYTO 16 | 489 | 520 |
|
|
| SYTO 17 | 619 | 638 |
|
|
| SYTO 61 | 618 | 650 |
|
|
| SYTO 9 | 483 | 500 |
|
|
| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
|
| SYTOX Green-DNA | 504 | 524 |
|
|
| TagBFP | 402 | 457 |
|
|
| TagCFP | 458 | 480 |
|
|
| Texas Red dextran | 592 | 613 |
|
|
| Texas Red DHPE | 584 | 608 |
|
|
| Texas Red-X® | 595 | 613 |
|
|
| TOTO-1 | 514 | 531 |
|
|
| TurboFP635(Katushka) | 591 | 638 |
|
|
| TurboGFP | 482 | 503 |
|
|
| Vybrant DyeCycle Green | 506 | 534 |
|
|
| Vybrant DyeCycle Violet | 369 | 437 |
|
|
| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
|
| X-Rhod-1 Indicator | 580 | 601 |
|
|
| YO-PRO-1 | 491 | 506 |
|
|
| YO-PRO-3 | 613 | 629 |
|
|
| YOYO-1 | 491 | 508 |
|
|
| YOYO-3 | 611 | 631 |
|
| Link | Description |
| Request a Drawing | Looking for an optical filter drawing file for this product? Request one now! |
| Request Custom Optical Filters | Contact us today to start a conversation about custom optical filters. |
| Access Optical Filter Warranty | Find our warranty in our Terms & Conditions of Sale. |
| Semrock Optical Filters RoHS Certificate | RoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances. |
| Semrock Optical Filters REACH Certificate | REACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry. |
| Download Optical Filters 101 Guide | Find an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now. |
| Request Clean Filter Checklist Poster | Looking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster. |
total selected
Clear selection
Select all 3 products from all pages.
FF01-432/523/702-25
FL-004189 / 0
- Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 546 nm & Tavg > 93% 604 – 800 nm
- Center Wavelengths of 432 nm, 522.8 nm & 702 nm and GMBWs of 36 nm, 46.5 nm & 196 nm
FF01-378/474/575-25
FL-004582 / 0
- Tavg > 85% 352 – 404 nm, Tavg > 93% 461 – 487.5 nm & Tavg > 93% 562.5 – 587.5 nm
- Center Wavelengths of 378 nm, 474 nm & 575 nm and GMBWs of 52 nm, 26.5 nm & 25 nm
FF409/493/596-Di02-25x36
FL-007086 / 0
- Dichroic Beamsplitter with edges at 409 nm, 493 nm & 596 nm
- Ravg > 95% 350 – 404 nm, Ravg > 95% 461 – 487.5 nm & Ravg > 95% 559.5 – 589.5 nm
- Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 546 nm & Tavg > 93% 604 – 850 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
| Link | Description |
| Request a Drawing | Looking for an optical filter drawing file for this product? Request one now! |
| Request Custom Optical Filters | Contact us today to start a conversation about custom optical filters. |
| Access Optical Filter Warranty | Find our warranty in our Terms & Conditions of Sale. |
| Semrock Optical Filters RoHS Certificate | RoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances. |
| Semrock Optical Filters REACH Certificate | REACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry. |
| Download Optical Filters 101 Guide | Find an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now. |
| Request Clean Filter Checklist Poster | Looking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster. |
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