Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.
This 5-filter "Pinkel" triple-band set is designed for imaging of a sample that is simultaneously labeled with DAPI, FITC, and Texas Red, (or other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines, including 365 nm DAPI LEDs, compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter and a triple-band emitter (these can be offered assembled in a microscope cube), plus three "no burn-out" single-band exciters that are intended to be mounted in the LED-based light engines, or in an exciter filter wheel or slider.
Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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2,703.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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Filter set mounted in microscope holders/cubes found below in related products section.
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FF01-432/523/702-25
FL-004189 / 0
- Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 546 nm & Tavg > 93% 604 – 800 nm
- Center Wavelengths of 432 nm, 522.8 nm & 702 nm and GMBWs of 36 nm, 46.5 nm & 196 nm
FF03-575/25-25
FL-004522 / 0
- Tavg > 93% 563 – 588 nm
- Center Wavelength of 575 nm and GMBW of 25 nm
- FWHM of 31.3 nm
FF01-378/52-25
FL-004583 / 0
- Tavg > 85% 352 – 404 nm
- Center Wavelength of 378 nm and GMBW of 52 nm
- FWHM of 54.6 nm
FF01-474/27-25
FL-004596 / 0
- Tavg > 93% 461.0 – 487.5 nm
- Center Wavelength of 474.3 nm and GMBW of 26.5 nm
- FWHM of 30.9 nm
FF409/493/596-Di02-25x36
FL-007086 / 0
- Dichroic Beamsplitter with edges at 409 nm, 493 nm & 596 nm
- Ravg > 95% 350 – 404 nm, Ravg > 95% 461 – 487.5 nm & Ravg > 95% 559.5 – 589.5 nm
- Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 546 nm & Tavg > 93% 604 – 850 nm
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
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| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
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| Acridine Orange (+DNA) | 502 | 526 |
|
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| Acridine Yellow | 470 | 550 |
|
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| Alexa Fluor® 405 | 401 | 422 |
|
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| Alexa Fluor® 430 | 431 | 541 |
|
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
|
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| Alexa Fluor® 568 | 579 | 603 |
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| Alexa Fluor® 594 | 590 | 618 |
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| Alexa Fluor® 660 | 663 | 691 |
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| AmCyan1 | 458 | 489 |
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| AsRed 2 | 576 | 592 |
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| ATTO 465 | 453 | 507 |
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| ATTO 488 | 500 | 525 |
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| ATTO 495 | 495 | 527 |
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| ATTO 565 | 563 | 592 |
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| ATTO 590 | 594 | 624 |
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| ATTO 594 | 601 | 624 |
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| BD Horizon V450 | 406 | 449 |
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| BFP (EBFP) | 380 | 440 |
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| BOBO™-1 | 461 | 484 |
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| BOBO™-3 | 570 | 605 |
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| BODIPY FL | 505 | 512 |
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| BODIPY FL-MeOH | 502 | 511 |
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| BODIPY TR-X phallacidin | 590 | 621 |
|
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| Calcein | 494 | 514 |
|
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| Calcium Crimson | 589 | 609 |
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| Carboxynaphthofluorescein | 599 | 674 |
|
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| Cascade Blue™ | 401 | 419 |
|
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| CellTrace BODIPY TR methyl ester | 598 | 625 |
|
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| CellTrace calcein violet | 400 | 452 |
|
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| CellTracker Red CMTPX | 586 | 614 |
|
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| CFP2 | 490 | 510 |
|
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| Cy2™ | 492 | 507 |
|
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| Cy3.5™ | 578 | 591 |
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| CyQUANT GR-DNA | 502 | 523 |
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| DAF-FM-NO | 495 | 519 |
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| DAPI | 359 | 461 |
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| DDAO | 648 | 657 |
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| Dendra2 (Green) | 491 | 507 |
|
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| DiO | 475 | 500 |
|
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| Dronpa | 502 | 516 |
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| DY-505-Phalloidin | 503 | 530 |
|
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| DY-590 | 582 | 599 |
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| DY-630 | 634 | 656 |
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| DyLight 405 | 398 | 420 |
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| DyLight 594 | 593 | 618 |
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| ecliptic pHluorin pH5.5 | 473 | 507 |
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| Emerald | 491 | 511 |
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| evoglow-Bs1 | 449 | 496 |
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| FITC (Fluorescein) | 495 | 519 |
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| Fluo-4 | 494 | 516 |
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| Fluorescein dextran | 501 | 524 |
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| Fluorescein-pH 8.0 | 489 | 517 |
|
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| Fluoro-Emerald | 494 | 518 |
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| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
|
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| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
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| FM 4-64 | 505 | 725 |
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| GFP (EGFP) | 489 | 511 |
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| Green 496 | 496 | 520 |
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| Green 500 | 501 | 524 |
|
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| HcRed1 | 588 | 618 |
|
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
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| HiLyte Fluor™ 405 | 403 | 428 |
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| HiLyte Fluor™ 488 | 497 | 526 |
|
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| HiLyte Fluor™ 594 | 592 | 616 |
|
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| KFP-Red | 575 | 599 |
|
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| LDS 751 | 560 | 711 |
|
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
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| LIVE-DEAD Fixable Red Dead Cell Stain | 595 | 613 |
|
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| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
|
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| LysoSensor Blue | 374 | 424 |
|
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| LysoSensor Green | 448 | 502 |
|
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| LysoTracker Green | 501 | 509 |
|
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| LysoTracker Red | 573 | 592 |
|
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| LysoTracker Yellow HCK-123 | 488 | 565 |
|
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| mApple | 566 | 594 |
|
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| Marina Blue | 363 | 461 |
|
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| mCherry | 587 | 610 |
|
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| mHoneyDew | 478 | 562 |
|
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| MitoTracker™ Green | 490 | 512 |
|
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| MitoTracker™ Red | 578 | 598 |
|
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| mPlum | 589 | 649 |
|
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| mRFP1 | 584 | 700 |
|
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| mRuby | 558 | 605 |
|
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| mStrawberry | 574 | 596 |
|
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| mTFP1 | 462 | 492 |
|
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| mWasabi | 493 | 509 |
|
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| NBD-X (MeOH) | 467 | 538 |
|
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
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| Nile blue-EtOH | 631 | 660 |
|
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| Nile red-phospolipid | 553 | 637 |
|
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| Orange 552 | 552 | 574 |
|
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| Oregon Green™ 488 | 498 | 526 |
|
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| Pacific Blue™ | 404 | 455 |
|
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| PerCP | 490 | 677 |
|
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| PicoGreen® | 502 | 522 |
|
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| PKH67 | 488 | 500 |
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| Propidium Iodide (PI) | 305 | 617 |
|
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| Qdot® 525 Nanocrystals | 300 | 525 |
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| Qdot® 545 Nanocrystals | 300 | 543 |
|
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| Qdot® 605 Nanocrystals | 300 | 602 |
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| Qdot® 625 Nanocrystals | 300 | 621 |
|
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| Qdot® 655 Nanocrystals | 300 | 654 |
|
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| Qdot® 705 Nanocrystals | 300 | 702 |
|
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| Qdot® 800 Nanocrystals | 300 | 792 |
|
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| ReAsH-CCPGCC | 592 | 606 |
|
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| Red 580 | 580 | 603 |
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| Rhodamine 110 | 497 | 519 |
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| Rhodamine Green | 497 | 523 |
|
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| Rhodamine Red-X | 573 | 591 |
|
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| Rhodol Green | 497 | 524 |
|
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| sgBFP™ | 387 | 451 |
|
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| sgGFP™ (super glow GFP) | 472 | 506 |
|
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| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
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| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
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| SNARF-1 514nm (ph 9.0) | 576 | 638 |
|
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| Sodium Green | 507 | 532 |
|
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| SpectrumBlue | 405 | 449 |
|
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| SpectrumGreen | 497 | 538 |
|
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| SpectrumRed | 587 | 615 |
|
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| Sulforhodamine 101-EtOH | 578 | 593 |
|
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
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| SYTO 11 | 506 | 525 |