Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.
This 7-filter "Sedat" quad-band set is designed for high-speed, sequential imaging of DAPI, FITC, and Texas Red (and other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines, including 365 nm DAPI LEDs, compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter with three single-band emitters intended to be mounted in filter wheels and three single-band exciters which can be installed into the LED light engine or mounted in filter wheels.
Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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This set is mounted in a Leica Microsystems DMi8 (“P-Cube”) Fluorescence Filter Holder, which is compatible with the DMi8 Leica microscope.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 3.5 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
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2-dodecylresorufin-lipid | 582 | 595 |
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5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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5-FAM (5-carboxyfluorescein) | 492 | 518 |
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5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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7-AAD (7-aminoactinomycin D) | 548 | 648 |
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Acridine Orange (+DNA) | 502 | 526 |
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Acridine Yellow | 470 | 550 |
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Alexa Fluor® 405 | 401 | 422 |
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Alexa Fluor® 430 | 431 | 541 |
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Alexa Fluor® 488 | 499 | 520 |
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Alexa Fluor® 500 | 503 | 525 |
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Alexa Fluor® 568 | 579 | 603 |
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Alexa Fluor® 594 | 590 | 618 |
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Alexa Fluor® 660 | 663 | 691 |
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AmCyan1 | 458 | 489 |
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AsRed 2 | 576 | 592 |
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ATTO 465 | 453 | 507 |
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ATTO 488 | 500 | 525 |
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ATTO 495 | 495 | 527 |
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ATTO 565 | 563 | 592 |
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ATTO 590 | 594 | 624 |
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ATTO 594 | 601 | 624 |
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BD Horizon V450 | 406 | 449 |
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BFP (EBFP) | 380 | 440 |
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BOBO™-1 | 461 | 484 |
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BOBO™-3 | 570 | 605 |
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BODIPY FL | 505 | 512 |
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BODIPY FL-MeOH | 502 | 511 |
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BODIPY TR-X phallacidin | 590 | 621 |
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BV421 - BD Horizon Brilliant™ Violet 421 | 407 | 421 |
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Calcein | 494 | 514 |
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Calcium Crimson | 589 | 609 |
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Carboxynaphthofluorescein | 599 | 674 |
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Cascade Blue™ | 401 | 419 |
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CellTrace BODIPY TR methyl ester | 598 | 625 |
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CellTrace calcein violet | 400 | 452 |
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CellTracker Red CMTPX | 586 | 614 |
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CFP2 | 490 | 510 |
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Cy2™ | 492 | 507 |
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Cy3.5™ | 578 | 591 |
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CyQUANT GR-DNA | 502 | 523 |
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DAF-FM-NO | 495 | 519 |
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DAPI | 359 | 461 |
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DDAO | 648 | 657 |
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Dendra2 (Green) | 491 | 507 |
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DiO | 475 | 500 |
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Dronpa | 502 | 516 |
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DY-505-Phalloidin | 503 | 530 |
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DY-590 | 582 | 599 |
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