This Full-multiband penta-band set is designed for imaging DAPI, FITC, TRITC, Cy5 and Cy7 (and other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in penta-band performance, and has been optimized for popular LED-based light engines including 365 nm DAPI LEDs. This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a penta-band beamsplitter, emitter, and exciter filter, all with "no burn-out" performance.
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FF01-378/474/554/635/735-25
FL-004024 / 0
- Tavg > 85% 353 – 403 nm, Tavg > 90% 462 – 486.5 nm, Tavg > 90% 544 – 565 nm, Tavg > 90% 627 – 643 nm & Tavg > 90% 722 – 748 nm
- Center Wavelengths of 378 nm, 474.3 nm, 554.5 nm, 635 nm & 735 nm and GMBWs of 50 nm, 24.5 nm, 21 nm, 16 nm & 26 nm
FF01-432/515/595/681/809-25
FL-004586 / 0
- Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 530 nm, Tavg > 93% 580 – 611 nm, Tavg > 93% 661 – 701 nm & Tavg > 93% 768.5 – 849.5 nm
- Center Wavelengths of 432 nm, 515 nm, 595 nm, 681 nm & 809 nm and GMBWs of 36 nm, 30.5 nm, 31 nm, 40 nm & 81 nm
FF409/493/573/652/759-Di01-25x36
FL-007112 / 0
- Dichroic Beamsplitter with edges at 409 nm, 493 nm, 573 nm, 652 nm & 759 nm
- Ravg > 95% 350 – 404 nm, Ravg > 95% 461 – 487.5 nm, Ravg > 95% 543 – 566 nm, Ravg > 95% 626 – 644 nm & Ravg > 95% 721 – 749 nm
- Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 530 nm, Tavg > 93% 580 – 611 nm, Tavg > 93% 661 – 701 nm & Tavg > 93% 768.5 – 849.5 nm
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This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
|
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| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
|
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
|
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| Acridine Orange (+DNA) | 502 | 526 |
|
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| Acridine Yellow | 470 | 550 |
|
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| Alexa Fluor® 405 | 401 | 422 |
|
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| Alexa Fluor® 430 | 431 | 541 |
|
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| Alexa Fluor® 488 | 499 | 520 |
|
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| Alexa Fluor® 500 | 503 | 525 |
|
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| Alexa Fluor® 546 | 556 | 572 |
|
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| Alexa Fluor® 555 | 553 | 565 |
|
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| Alexa Fluor® 568 | 579 | 603 |
|
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| Alexa Fluor® 633 | 631 | 647 |
|
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| Alexa Fluor® 647 | 653 | 668 |
|
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| Alexa Fluor® 660 | 663 | 691 |
|
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| Alexa Fluor® 680 | 679 | 702 |
|
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| Alexa Fluor® 700 | 696 | 719 |
|
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| Alexa Fluor® 750 | 751 | 776 |
|
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| Alexa Fluor® 790 | 782 | 805 |
|
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| AmCyan1 | 458 | 489 |
|
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| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
|
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| APC | 651 | 660 |
|
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| APC (Allophycocyanin) | 651 | 660 |
|
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| AsRed 2 | 576 | 592 |
|
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| ATTO 465 | 453 | 507 |
|
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| ATTO 488 | 500 | 525 |
|
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| ATTO 495 | 495 | 527 |
|
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| ATTO 550 | 553 | 576 |
|
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| ATTO 565 | 563 | 592 |
|
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| ATTO 633 | 629 | 658 |
|
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| ATTO 635 | 635 | 658 |
|
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| ATTO 647 | 644 | 670 |
|
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| ATTO 647N | 645 | 668 |
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| ATTO 655 | 663 | 683 |
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| ATTO 680 | 680 | 700 |
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| ATTO 700 | 699 | 719 |
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| ATTO 725 | 727 | 754 |
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| ATTO 740 | 740 | 764 |
|
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| BD Horizon V450 | 406 | 449 |
|
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| BFP (EBFP) | 380 | 440 |
|
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| BOBO™-1 | 461 | 484 |
|
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| BOBO™-3 | 570 | 605 |
|
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| BODIPY 650/665-X | 646 | 664 |
|
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| BODIPY FL | 505 | 512 |
|
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| BODIPY FL-MeOH | 502 | 511 |
|
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| BODIPY TMR-X | 544 | 573 |
|
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| BODIPY TR-X (MeOH) | 544 | 573 |
|
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| Calcein | 494 | 514 |
|
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| Calcium Crimson | 589 | 609 |
|
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| Calcium Orange | 589 | 609 |
|
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| Carboxynaphthofluorescein | 599 | 674 |
|
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| Cascade Blue™ | 401 | 419 |
|
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| CellTrace calcein violet | 400 | 452 |
|
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| CellTracker Red CMTPX | 586 | 614 |
|
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| CFP2 | 490 | 510 |
|
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| Cy2™ | 492 | 507 |
|
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| Cy3.5™ | 578 | 591 |
|
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| Cy3™ | 554 | 566 |
|
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| Cy5.5™ | 672 | 690 |
|
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| Cy5™ | 649 | 666 |
|
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| Cy7™ | 753 | 775 |
|
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| CyQUANT GR-DNA | 502 | 523 |
|
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| DAF-FM-NO | 495 | 519 |
|
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| DAPI | 359 | 461 |
|
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| DDAO | 648 | 657 |
|
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| Dendra2 (Green) | 491 | 507 |
|
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| Dendra2 (Red) | 554 | 569 |
|
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| DiD (DiIC18(5)) - Lipophilic Tracer | 648 | 670 |
|
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| DiI | 551 | 569 |
|
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| DiO | 475 | 500 |
|
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| DiR | 750 | 782 |
|
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| Dronpa | 502 | 516 |
|
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| DsRed | 559 | 583 |
|
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| DsRed-Express | 556 | 584 |
|
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| dTomato | 556 | 582 |
|
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| DY-505-Phalloidin | 503 | 530 |
|
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| DY-590 | 582 | 599 |
|
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| DY-630 | 634 | 656 |
|
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| DY-635 | 646 | 670 |
|
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| DY-781 | 784 | 802 |
|
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| DyLight 405 | 398 | 420 |
|
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| DyLight 649 | 651 | 673 |
|
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| DyLight 680 | 676 | 705 |
|
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| DyLight 750 | 751 | 778 |
|
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| DyLight 800 | 777 | 794 |
|
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| ecliptic pHluorin pH5.5 | 473 | 507 |
|
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| Emerald | 491 | 511 |
|
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| Ethidium bromide | 518 | 603 |
|
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| Ethidium homodimer | 527 | 617 |
|
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| evoglow-Bs1 | 449 | 496 |
|
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| FITC (Fluorescein) | 495 | 519 |
|
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| Fluo-4 | 494 | 516 |
|
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| Fluorescein dextran | 501 | 524 |
|
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| Fluorescein-pH 8.0 | 489 | 517 |
|
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| Fluoro-Emerald | 494 | 518 |
|
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| FluoSpheres Dark Red fluorescent microspheres | 656 | 683 |
|
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| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
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| GFP (EGFP) | 489 | 511 |
|
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| Gold 550 | 551 | 564 |
|
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| Green 496 | 496 | 520 |
|
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| Green 500 | 501 | 524 |
|
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| HCS CellMask Red cytoplasmic | 622 | 644 |
|
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| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
|
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
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| HiLyte Fluor™ 405 | 403 | 428 |
|
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| HiLyte Fluor™ 488 | 497 | 526 |
|
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| HiLyte Fluor™ 555 | 550 | 566 |
|
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| HiLyte Fluor™ 647 | 649 | 673 |
|
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| HiLyte Fluor™ 750 | 750 | 778 |
|
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| IRDye 800 CW-MeOH | 778 | 801 |
|
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| IRDye 800CW | 775 | 792 |
|
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| IRDye 800CW-NHS Ester | 775 | 792 |
|
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| IRDye 800Phosphoramidite | 257 | 809 |
|
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| IRDye 800RS | 787 | 812 |
|
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| KFP-Red | 575 | 599 |
|
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| LDS 751 | 560 | 711 |
|
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| LIVE-DEAD Fixable Far Red Dead Cell Stain | 653 | 668 |
|
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
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| LIVE-DEAD Fixable Near-IR Dead Cell Stain | 752 | 776 |
|
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| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
|
|
| LOLO-1 | 568 | 580 |
|
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| LysoSensor Blue | 374 | 424 |
|
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| LysoSensor Green | 448 | 502 |
|
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| LysoTracker Green | 501 | 509 |
|
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| LysoTracker Red | 573 | 592 |
|
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| LysoTracker Yellow HCK-123 | 488 | 565 |
|
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| Magnesium Orange | 550 | 574 |
|
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| mApple | 566 | 594 |
|
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| Marina Blue | 363 | 461 |
|
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| mCherry | 587 | 610 |
|
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| Merocyanine 540 | 559 | 579 |
|
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| mHoneyDew | 478 | 562 |
|
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| MitoTracker™ Deep Red | 641 | 662 |
|
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| MitoTracker™ Green | 490 | 512 |
|
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| MitoTracker™ Orange | 551 | 575 |
|
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| MitoTracker™ Red | 578 | 598 |
|
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| mOrange | 546 | 562 |
|
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| mRFP | 555 | 583 |
|
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| mRFP1 | 584 | 700 |
|
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| mRuby | 558 | 605 |
|
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| mStrawberry | 574 | 596 |
|
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| mTangerine | 568 | 585 |
|
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| mTFP1 | 462 | 492 |
|
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| mWasabi | 493 | 509 |
|
|
| NBD-X (MeOH) | 467 | 538 |
|
|
| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
|
| Nile blue-EtOH | 631 | 660 |
|
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| Nile red-triglyceride | 510 | 583 |
|
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| OFP | 547 | 567 |
|
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| Oregon Green™ 488 | 498 | 526 |
|
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| Pacific Blue™ | 404 | 455 |
|
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| pHrodo™, succinimidyl ester | 560 | 587 |
|
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| PicoGreen® | 502 | 522 |
|
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| PKH67 | 488 | 500 |
|
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| POPO-3 | 533 | 574 |
|
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| PO-PRO-3 | 539 | 570 |
|
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| Propidium Iodide (PI) | 305 | 617 |
|
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| Pro-Q Diamond | 556 | 583 |
|
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| Qdot® 525 Nanocrystals | 300 | 525 |
|
|
| Qdot® 585 Nanocrystals | 300 | 588 |
|
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| Qdot® 605 Nanocrystals | 300 | 602 |
|
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| Qdot® 705 Nanocrystals | 300 | 702 |
|
|
| Qdot® 800 Nanocrystals | 300 | 792 |
|
|
| Quasar® 705 | 688 | 706 |
|
|
| ReAsH-CCPGCC | 592 | 606 |
|
|
| Red 580 | 580 | 603 |
|
|
| Red 650 | 649 | 665 |
|
|
| Resorufin | 571 | 584 |
|
|
| Rhod-2 | 553 | 577 |
|
|
| Rhodamine 110 | 497 | 519 |
|
|
| Rhodamine Green | 497 | 523 |
|
|
| Rhodamine phalloidin | 558 | 575 |
|
|
| Rhodamine Red-X | 573 | 591 |
|
|
| Rhodol Green | 497 | 524 |
|
|
| R-phycoerythrin (PE) | 565 | 576 |
|
|
| sgBFP™ | 387 | 451 |
|
|
| sgGFP™ (super glow GFP) | 472 | 506 |
|
|
| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
|
| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
|
| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
|
| Sodium Green | 507 | 532 |
|
|
| SpectrumBlue | 405 | 449 |
|
|
| SpectrumFRed (Far Red) | 650 | 673 |
|
|
| SpectrumGreen | 497 | 538 |
|
|
| SpectrumOrange | 554 | 587 |
|
|
| Sulforhodamine 101-EtOH | 578 | 593 |
|
|
| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
|
| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
|
| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
|
| SYTO 11 | 506 | 525 |
|
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| SYTO 13 | 448 | 506 |
|
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| SYTO 16 | 489 | 520 |
|
|
| SYTO 59 | 622 | 644 |
|
|
| SYTO 60 | 650 | 681 |
|
|
| SYTO 61 | 618 | 650 |
|
|
| SYTO 82 | 540 | 559 |
|
|
| SYTO 9 | 483 | 500 |
|
|
| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
|
| SYTOX Green-DNA | 504 | 524 |
|
|
| SYTOX Orange | 547 | 570 |
|
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| SYTOX Red-DNA | 640 | 658 |
|
|
| TagBFP | 402 | 457 |
|
|
| TagCFP | 458 | 480 |
|
|
| Tetramethylrhodamine dextran | 554 | 582 |
|
|
| Texas Red DHPE | 584 | 608 |
|
|
| TO-PRO-3 | 642 | 657 |
|
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| TOTO-1 | 514 | 531 |
|