BrightLine® full-multiband filter set, optimized for DAPI, FITC, TRITC, Cy5, & Cy7 and other like fluorophores, illuminated with LED-based light engines

Semrock Part Number: LED-DA/FI/TR/Cy5/Cy7-A-000-ZERO

IDEX Part Number: S-001105  /

Semrock
This Full-multiband penta-band set is designed for imaging DAPI, FITC, TRITC, Cy5 and Cy7 (and other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in penta-band performance, and has been optimized for popular LED-based light engines including 365 nm DAPI LEDs. This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a penta-band beamsplitter, emitter, and exciter filter, all with "no burn-out" performance.
  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 2,344.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
Set Components

FF01-378/474/554/635/735-25

FL-004024

Each
  • Tavg > 85% 353 – 403 nm, Tavg > 90% 462 – 486.5 nm, Tavg > 90% 544 – 565 nm, Tavg > 90% 627 – 643 nm & Tavg > 90% 722 – 748 nm
  • Center Wavelengths of 378 nm, 474.3 nm, 554.5 nm, 635 nm & 735 nm and GMBWs of 50 nm, 24.5 nm, 21 nm, 16 nm & 26 nm

FL-004024

FL-004124

FL-006901

FF01-378/474/554/635/735-25

FL-004024 / 0

Out of Stock Backordered In Stock
  • Tavg > 85% 353 – 403 nm, Tavg > 90% 462 – 486.5 nm, Tavg > 90% 544 – 565 nm, Tavg > 90% 627 – 643 nm & Tavg > 90% 722 – 748 nm
  • Center Wavelengths of 378 nm, 474.3 nm, 554.5 nm, 635 nm & 735 nm and GMBWs of 50 nm, 24.5 nm, 21 nm, 16 nm & 26 nm

FF01-432/515/595/681/809-25

FL-004586 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 530 nm, Tavg > 93% 580 – 611 nm, Tavg > 93% 661 – 701 nm & Tavg > 93% 768.5 – 849.5 nm
  • Center Wavelengths of 432 nm, 515 nm, 595 nm, 681 nm & 809 nm and GMBWs of 36 nm, 30.5 nm, 31 nm, 40 nm & 81 nm

FF409/493/573/652/759-Di01-25x36

FL-007112 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edges at 409 nm, 493 nm, 573 nm, 652 nm & 759 nm
  • Ravg > 95% 350 – 404 nm, Ravg > 95% 461 – 487.5 nm, Ravg > 95% 543 – 566 nm, Ravg > 95% 626 – 644 nm & Ravg > 95% 721 – 749 nm
  • Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 530 nm, Tavg > 93% 580 – 611 nm, Tavg > 93% 661 – 701 nm & Tavg > 93% 768.5 – 849.5 nm

LED-DA/FI/TR/Cy5/Cy7-A-NT2-ZERO

S-434268 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope. This set is mounted in a Nikon NT2 Large Field of View Fluorescence Filter Holder, which is compatible with Nikon Eclipse Ti2 microscope.  

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TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-carboxynapthofluorescein (pH 10) 555 615
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
Acridine Orange (+DNA) 502 526
Acridine Yellow 470 550
Alexa Fluor® 405 401 422
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 546 556 572
Alexa Fluor® 555 553 565
Alexa Fluor® 568 579 603
Alexa Fluor® 633 631 647
Alexa Fluor® 647 653 668
Alexa Fluor® 660 663 691
Alexa Fluor® 680 679 702
Alexa Fluor® 700 696 719
Alexa Fluor® 750 751 776
Alexa Fluor® 790 782 805
AmCyan1 458 489
Amplex UltraRed peroxidation product (pH 7.5) 568 581
APC 651 660
APC (Allophycocyanin) 651 660
AsRed 2 576 592
ATTO 465 453 507
ATTO 488 500 525
ATTO 495 495 527
ATTO 550 553 576
ATTO 565 563 592
ATTO 633 629 658
ATTO 635 635 658
ATTO 647 644 670
ATTO 647N 645 668
ATTO 655 663 683
ATTO 680 680 700
ATTO 700 699 719
ATTO 725 727 754
ATTO 740 740 764
BD Horizon V450 406 449
BFP (EBFP) 380 440
BOBO™-1 461 484
BOBO™-3 570 605
BODIPY 650/665-X 646 664
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
BODIPY TMR-X 544 573
BODIPY TR-X (MeOH) 544 573
Calcein 494 514
Calcium Crimson 589 609
Calcium Orange 589 609
Carboxynaphthofluorescein 599 674
Cascade Blue™ 401 419
CellTrace calcein violet 400 452
CellTracker Red CMTPX 586 614
CFP2 490 510
Cy2™ 492 507
Cy3.5™ 578 591
Cy3™ 554 566
Cy5.5™ 672 690
Cy5™ 649 666
Cy7™ 753 775
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
DAPI 359 461
DDAO 648 657
Dendra2 (Green) 491 507
Dendra2 (Red) 554 569
DiD (DiIC18(5)) - Lipophilic Tracer 648 670
DiI 551 569
DiO 475 500
DiR 750 782
Dronpa 502 516
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-505-Phalloidin 503 530
DY-590 582 599
DY-630 634 656
DY-635 646 670
DY-781 784 802
DyLight 405 398 420
DyLight 649 651 673
DyLight 680 676 705
DyLight 750 751 778
DyLight 800 777 794
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
Ethidium bromide 518 603
Ethidium homodimer 527 617
evoglow-Bs1 449 496
FITC (Fluorescein) 495 519
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Dark Red fluorescent microspheres 656 683
FluoSpheres Red fluorescent microspheres 577 607
FluoSpheres Yellow-Green fluorescent microspheres 503 514
GFP (EGFP) 489 511
Gold 550 551 564
Green 496 496 520
Green 500 501 524
HCS CellMask Red cytoplasmic 622 644
HCS LipidTOX Deep Red neutral lipid stain 635 652
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 405 403 428
HiLyte Fluor™ 488 497 526
HiLyte Fluor™ 555 550 566
HiLyte Fluor™ 647 649 673
HiLyte Fluor™ 750 750 778
IRDye 800 CW-MeOH 778 801
IRDye 800CW 775 792
IRDye 800CW-NHS Ester 775 792
IRDye 800Phosphoramidite 257 809
IRDye 800RS 787 812
KFP-Red 575 599
LDS 751 560 711
LIVE-DEAD Fixable Far Red Dead Cell Stain 653 668
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LIVE-DEAD Fixable Near-IR Dead Cell Stain 752 776
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
LOLO-1 568 580
LysoSensor Blue 374 424
LysoSensor Green 448 502
LysoTracker Green 501 509
LysoTracker Red 573 592
LysoTracker Yellow HCK-123 488 565
Magnesium Orange 550 574
mApple 566 594
Marina Blue 363 461
mCherry 587 610
Merocyanine 540 559 579
mHoneyDew 478 562
MitoTracker™ Deep Red 641 662
MitoTracker™ Green 490 512
MitoTracker™ Orange 551 575
MitoTracker™ Red 578 598
mOrange 546 562
mRFP 555 583
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596