BrightLine® Pinkel filter set, optimized for DAPI, FITC, TRITC, Cy5 & Cy7 and other like fluorophores, illuminated with LED-based light sources

Semrock Part Number: LED-DA/FI/TR/Cy5/Cy7-5X-A-NQF-ZERO

IDEX Part Number: S-001668  /

Semrock

This 7-filter "Pinkel" penta-band set is designed for imaging of a sample that is simultaneously labeled with DAPI, FITC, TRITC, Cy5, and Cy7 (or other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in penta-band performance, and has been optimized for popular LED-based light sources. This set has been designed to deliver significant fluorescence signal improvements for LED-based light sources compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a penta-band beamsplitter and a penta-band emitter (these can be offered assembled in a microscope cube), plus five "no burn-out" single-band exciters that are intended to be mounted in the LED based light source, or in an exciter filter wheel or slider.

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  • Price Not Available.
  • 3,883.00 USD Each
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FF01-378/52-25

FL-004583 / 0

Out of Stock Backordered In Stock
  • Tavg > 85% 352 – 404 nm
  • Center Wavelength of 378 nm and GMBW of 52 nm
  • FWHM of 54.6 nm

FF01-432/515/595/681/809-25

FL-004586 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 530 nm, Tavg > 93% 580 – 611 nm, Tavg > 93% 661 – 701 nm & Tavg > 93% 768.5 – 849.5 nm
  • Center Wavelengths of 432 nm, 515 nm, 595 nm, 681 nm & 809 nm and GMBWs of 36 nm, 30.5 nm, 31 nm, 40 nm & 81 nm

FF01-474/27-25

FL-004596 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 461.0 – 487.5 nm
  • Center Wavelength of 474.3 nm and GMBW of 26.5 nm
  • FWHM of 30.9 nm

FF01-554/23-25

FL-004612 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 543.0 – 566.0 nm
  • Center Wavelength of 554.5 nm and GMBW of 23.0 nm
  • FWHM of 28.1 nm

FF01-635/18-25

FL-004696 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 626 – 644 nm
  • Center Wavelength of 635 nm and GMBW of 18 nm
  • FWHM of 23.9 nm

FF01-735/28-25

FL-004707 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 721 – 749 nm
  • Center Wavelength of 735 nm and GMBW of 28 nm
  • FWHM of 33.1 nm

FF409/493/573/652/759-Di01-25x36

FL-007112 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edges at 409 nm, 493 nm, 573 nm, 652 nm & 759 nm
  • Ravg > 95% 350 – 404 nm, Ravg > 95% 461 – 487.5 nm, Ravg > 95% 543 – 566 nm, Ravg > 95% 626 – 644 nm & Ravg > 95% 721 – 749 nm
  • Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 530 nm, Tavg > 93% 580 – 611 nm, Tavg > 93% 661 – 701 nm & Tavg > 93% 768.5 – 849.5 nm

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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm

1.1 mm
(Can accept a 2 mm dichroic)
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
2-dodecylresorufin-lipid 582 595
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518