This 7-filter "Pinkel" penta-band set is designed for imaging of a sample that is simultaneously labeled with DAPI, FITC, TRITC, Cy5, and Cy7 (or other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in penta-band performance, and has been optimized for popular LED-based light sources. This set has been designed to deliver significant fluorescence signal improvements for LED-based light sources compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a penta-band beamsplitter and a penta-band emitter (these can be offered assembled in a microscope cube), plus five "no burn-out" single-band exciters that are intended to be mounted in the LED based light source, or in an exciter filter wheel or slider.
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This set is mounted in a Leica Microsystems DMi8 (“P-Cube”) Fluorescence Filter Holder, which is compatible with the DMi8 Leica microscope.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 3.5 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
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1,8-ANS | 372 | 477 |
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2-dodecylresorufin-lipid | 582 | 595 |
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4-methylumbelliferone | 357 | 441 |
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5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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5-FAM (5-carboxyfluorescein) | 492 | 518 |
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5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
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7-AAD (7-aminoactinomycin D) | 548 | 648 |
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7-hydroxy-4-methylcoumarin | 360 | 442 |
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Acridine Orange (+DNA) | 502 | 526 |
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Alexa Fluor® 405 | 401 | 422 |
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Alexa Fluor® 488 | 499 | 520 |
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Alexa Fluor® 500 | 503 | 525 |
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Alexa Fluor® 546 | 556 | 572 |
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Alexa Fluor® 555 | 553 | 565 |
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Alexa Fluor® 568 | 579 | 603 |
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Alexa Fluor® 594 | 590 | 618 |
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Alexa Fluor® 647 | 653 | 668 |
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Alexa Fluor® 660 | 663 | 691 |
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Alexa Fluor® 680 | 679 | 702 |
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Alexa Fluor® 750 | 751 | 776 |
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Alexa Fluor® 790 | 782 | 805 |
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AMCA (Aminomethylcoumarin) | 350 | 448 |
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AmCyan1 | 458 | 489 |
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Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
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APC | 651 | 660 |
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APC (Allophycocyanin) | 651 | 660 |
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