BrightLine® Pinkel filter set, optimized for DAPI, FITC, TRITC, Cy5 & Cy7 and other like fluorophores, illuminated with LED-based light sources

Semrock Part Number: LED-DA/FI/TR/Cy5/Cy7-5X-A-LDMP-ZERO

IDEX Part Number: S-001666  /

Semrock

This 7-filter "Pinkel" penta-band set is designed for imaging of a sample that is simultaneously labeled with DAPI, FITC, TRITC, Cy5, and Cy7 (or other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in penta-band performance, and has been optimized for popular LED-based light sources. This set has been designed to deliver significant fluorescence signal improvements for LED-based light sources compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a penta-band beamsplitter and a penta-band emitter (these can be offered assembled in a microscope cube), plus five "no burn-out" single-band exciters that are intended to be mounted in the LED based light source, or in an exciter filter wheel or slider.

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  • Price Not Available.
  • 3,933.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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FF01-378/52-25

FL-004583 / 0

Out of Stock Backordered In Stock
  • Tavg > 85% 352 – 404 nm
  • Center Wavelength of 378 nm and GMBW of 52 nm
  • FWHM of 54.6 nm

FF01-432/515/595/681/809-25

FL-004586 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 530 nm, Tavg > 93% 580 – 611 nm, Tavg > 93% 661 – 701 nm & Tavg > 93% 768.5 – 849.5 nm
  • Center Wavelengths of 432 nm, 515 nm, 595 nm, 681 nm & 809 nm and GMBWs of 36 nm, 30.5 nm, 31 nm, 40 nm & 81 nm

FF01-474/27-25

FL-004596 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 461.0 – 487.5 nm
  • Center Wavelength of 474.3 nm and GMBW of 26.5 nm
  • FWHM of 30.9 nm

FF01-554/23-25

FL-004612 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 543.0 – 566.0 nm
  • Center Wavelength of 554.5 nm and GMBW of 23.0 nm
  • FWHM of 28.1 nm

FF01-635/18-25

FL-004696 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 626 – 644 nm
  • Center Wavelength of 635 nm and GMBW of 18 nm
  • FWHM of 23.9 nm

FF01-735/28-25

FL-004707 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 721 – 749 nm
  • Center Wavelength of 735 nm and GMBW of 28 nm
  • FWHM of 33.1 nm

FF409/493/573/652/759-Di01-25x36

FL-007112 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edges at 409 nm, 493 nm, 573 nm, 652 nm & 759 nm
  • Ravg > 95% 350 – 404 nm, Ravg > 95% 461 – 487.5 nm, Ravg > 95% 543 – 566 nm, Ravg > 95% 626 – 644 nm & Ravg > 95% 721 – 749 nm
  • Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 530 nm, Tavg > 93% 580 – 611 nm, Tavg > 93% 661 – 701 nm & Tavg > 93% 768.5 – 849.5 nm

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This set is mounted in a Leica Microsystems DMi8 (“P-Cube”) Fluorescence Filter Holder, which is compatible with the DMi8 Leica microscope.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
2-dodecylresorufin-lipid 582 595
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
7-AAD (7-aminoactinomycin D) 548 648
7-hydroxy-4-methylcoumarin 360 442
Acridine Orange (+DNA) 502 526
Alexa Fluor® 405 401 422
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 546 556 572
Alexa Fluor® 555 553 565
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
Alexa Fluor® 647 653 668
Alexa Fluor® 660 663 691
Alexa Fluor® 680 679 702
Alexa Fluor® 750 751 776
Alexa Fluor® 790 782 805
AMCA (Aminomethylcoumarin) 350 448
AmCyan1 458 489
Amplex UltraRed peroxidation product (pH 7.5) 568 581
APC 651 660
APC (Allophycocyanin) 651 660