This 7-filter "Pinkel" penta-band set is designed for imaging of a sample that is simultaneously labeled with DAPI, FITC, TRITC, Cy5, and Cy7 (or other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in penta-band performance, and has been optimized for popular LED-based light sources. This set has been designed to deliver significant fluorescence signal improvements for LED-based light sources compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a penta-band beamsplitter and a penta-band emitter (these can be offered assembled in a microscope cube), plus five "no burn-out" single-band exciters that are intended to be mounted in the LED based light source, or in an exciter filter wheel or slider.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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3,863.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-378/52-25
FL-004583 / 0
- Tavg > 85% 352 – 404 nm
- Center Wavelength of 378 nm and GMBW of 52 nm
- FWHM of 54.6 nm
FF01-432/515/595/681/809-25
FL-004586 / 0
- Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 530 nm, Tavg > 93% 580 – 611 nm, Tavg > 93% 661 – 701 nm & Tavg > 93% 768.5 – 849.5 nm
- Center Wavelengths of 432 nm, 515 nm, 595 nm, 681 nm & 809 nm and GMBWs of 36 nm, 30.5 nm, 31 nm, 40 nm & 81 nm
FF01-474/27-25
FL-004596 / 0
- Tavg > 93% 461.0 – 487.5 nm
- Center Wavelength of 474.3 nm and GMBW of 26.5 nm
- FWHM of 30.9 nm
FF01-554/23-25
FL-004612 / 0
- Tavg > 93% 543.0 – 566.0 nm
- Center Wavelength of 554.5 nm and GMBW of 23.0 nm
- FWHM of 28.1 nm
FF01-635/18-25
FL-004696 / 0
- Tavg > 93% 626 – 644 nm
- Center Wavelength of 635 nm and GMBW of 18 nm
- FWHM of 23.9 nm
FF01-735/28-25
FL-004707 / 0
- Tavg > 93% 721 – 749 nm
- Center Wavelength of 735 nm and GMBW of 28 nm
- FWHM of 33.1 nm
FF409/493/573/652/759-Di01-25x36
FL-007112 / 0
- Dichroic Beamsplitter with edges at 409 nm, 493 nm, 573 nm, 652 nm & 759 nm
- Ravg > 95% 350 – 404 nm, Ravg > 95% 461 – 487.5 nm, Ravg > 95% 543 – 566 nm, Ravg > 95% 626 – 644 nm & Ravg > 95% 721 – 749 nm
- Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 530 nm, Tavg > 93% 580 – 611 nm, Tavg > 93% 661 – 701 nm & Tavg > 93% 768.5 – 849.5 nm
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This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 21.8 mm | 5.0 mm |
| Emitter (Diameter) | 23.3 mm | 5.0 mm |
| Dichroic (L × W) | 22.0 mm × 29.0 mm | 1.1 mm |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
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| 1,8-ANS | 372 | 477 |
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| 2-dodecylresorufin-lipid | 582 | 595 |
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| 4-methylumbelliferone | 357 | 441 |
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
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| 7-hydroxy-4-methylcoumarin | 360 | 442 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Alexa Fluor® 405 | 401 | 422 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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| Alexa Fluor® 546 | 556 | 572 |
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| Alexa Fluor® 555 | 553 | 565 |
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| Alexa Fluor® 568 | 579 | 603 |
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| Alexa Fluor® 594 | 590 | 618 |
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| Alexa Fluor® 647 | 653 | 668 |
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| Alexa Fluor® 660 | 663 | 691 |
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| Alexa Fluor® 680 | 679 | 702 |
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| Alexa Fluor® 750 | 751 | 776 |
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| Alexa Fluor® 790 | 782 | 805 |
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| AMCA (Aminomethylcoumarin) | 350 | 448 |
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| AmCyan1 | 458 | 489 |
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| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
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| APC | 651 | 660 |
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| APC (Allophycocyanin) | 651 | 660 |
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