BrightLine® Sedat filter set, optimized for DAPI, FITC, TRITC, Cy5 & Cy7 and other like fluorophores, illuminated with LED-based light engines

Semrock Part Number: LED-DA/FI/TR/Cy5/Cy7-5X5M-A-000

IDEX Part Number: S-000648  /

Semrock

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This 11-filter "Sedat" penta-band set is designed for high-speed, sequential imaging of DAPI, FITC, TRITC, Cy5, and Cy7 (and other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a quad-band beamsplitter with four single-band emitters intended to be mounted in filter wheels and four single-band exciters which can be installed into the LED light engine or mounted in filter wheels.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight spectral plotting & analysis tool.

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  • Price Not Available.
  • 4,495.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
Set Components

FF01-378/52-25

FL-004583

Each
  • Tavg > 85% 352 – 404 nm
  • Center Wavelength of 378 nm and GMBW of 52 nm
  • FWHM of 54.6 nm

FL-004583

FF01-432/36-25

FL-004373

Each
  • Tavg > 93% 414 – 450 nm
  • Center Wavelength of 432 nm and GMBW of 36 nm
  • FWHM of 39.6 nm

FL-004373

FF01-474/27-25

FL-004596

Each
  • Tavg > 93% 461.0 – 487.5 nm
  • Center Wavelength of 474.3 nm and GMBW of 26.5 nm
  • FWHM of 30.9 nm

FL-004596

FF01-515/30-25

FL-004382

Each
  • Tavg > 93% 499.5 – 530 nm
  • Center Wavelength of 514.8 nm and GMBW of 30.5 nm
  • FWHM of 35 nm

FL-004382

FF01-554/23-25

FL-004612

Each
  • Tavg > 93% 543.0 – 566.0 nm
  • Center Wavelength of 554.5 nm and GMBW of 23.0 nm
  • FWHM of 28.1 nm

FL-004612

FF01-595/31-25

FL-004391

Each
  • Tavg > 93% 580 – 611 nm
  • Center Wavelength of 595.5 nm and GMBW of 31 nm
  • FWHM of 36.7 nm

FL-004391

FF01-635/18-25

FL-004696

Each
  • Tavg > 93% 626 – 644 nm
  • Center Wavelength of 635 nm and GMBW of 18 nm
  • FWHM of 23.9 nm

FL-004696

FF01-680/42-25

FL-004700

Each
  • Tavg > 93% 659.5 – 701 nm
  • Center Wavelength of 680.3 nm and GMBW of 41.5 nm
  • FWHM of 47 nm

FL-004700

FF01-735/28-25

FL-004707

Each
  • Tavg > 93% 721 – 749 nm
  • Center Wavelength of 735 nm and GMBW of 28 nm
  • FWHM of 33.1 nm

FL-004707

FF02-809/81-25

FL-004520

Each
  • Tavg > 93% 768.5 – 849.5 nm
  • Center Wavelength of 809 nm and GMBW of 81 nm
  • FWHM of 88.4 nm

FL-004520

FF409/493/573/652/759-Di01-25x36

FL-007112

Each
  • Dichroic Beamsplitter with edges at 409 nm, 493 nm, 573 nm, 652 nm & 759 nm
  • Ravg > 95% 350 – 404 nm, Ravg > 95% 461 – 487.5 nm, Ravg > 95% 543 – 566 nm, Ravg > 95% 626 – 644 nm & Ravg > 95% 721 – 749 nm
  • Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 530 nm, Tavg > 93% 580 – 611 nm, Tavg > 93% 661 – 701 nm & Tavg > 93% 768.5 – 849.5 nm

FL-007112

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

LED-DA/FI/TR/Cy5/Cy7-5X-A-000

S-000645 / 0

Out of Stock Backordered In Stock
7-filter "Pinkel" penta-band set with 5 exciters, a penta-band emitter and a penta-band dichroic beamsplitter. Ideal for use with: Blue: DAPI; Green: FITC , GFP ; Orange: TRITC ; Red: Cy5; Red/ Near-IR: Cy7

LED-DA/FI/TR/Cy5/Cy7-5X5M-A-NQF

S-002836 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

LED-DA/FI/TR/Cy5/Cy7-5X5M-A-NTE

S-002837 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

LED-DA/FI/TR/Cy5/Cy7-5X5M-A-OMF

S-000334 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

LED-DA/FI/TR/Cy5/Cy7-5X5M-A-ZHE

S-002842 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

LED-DA/FI/TR/Cy5/Cy7-5X5M-A-OFF

S-002838 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

LED-DA/FI/TR/Cy5/Cy7-5X5M-A-LDMK

S-002833 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6