This Full-multiband quad-band set is designed for imaging DAPI, FITC, TRITC, and Cy5 (and other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in quad-band performance, and has been optimized for popular LED-based light engines including 365 nm DAPI LEDs. This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a quad-band beamsplitter, emitter, and exciter filter, all with "no burn-out" performance.
Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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FL-004669 / 0
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This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 3.5 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
Topic | Description |
Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
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2-dodecylresorufin-lipid | 582 | 595 |
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5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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5-FAM (5-carboxyfluorescein) | 492 | 518 |
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5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
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Acridine Orange (+DNA) | 502 | 526 |
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Acridine Yellow | 470 | 550 |
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Alexa Fluor® 405 | 401 | 422 |
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Alexa Fluor® 430 | 431 | 541 |
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Alexa Fluor® 488 | 499 | 520 |
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Alexa Fluor® 500 | 503 | 525 |
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Alexa Fluor® 546 | 556 | 572 |
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Alexa Fluor® 555 | 553 | 565 |
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Alexa Fluor® 568 | 579 | 603 |
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Alexa Fluor® 633 | 631 | 647 |
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Alexa Fluor® 647 | 653 | 668 |
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Alexa Fluor® 660 | 663 | 691 |
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Alexa Fluor® 680 | 679 | 702 |
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Alexa Fluor® 700 | 696 | 719 |
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AmCyan1 | 458 | 489 |
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Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
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APC | 651 | 660 |
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APC (Allophycocyanin) | 651 | 660 |
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AsRed 2 | 576 | 592 |
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ATTO 465 | 453 | 507 |
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ATTO 488 | 500 | 525 |
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ATTO 495 | 495 | 527 |
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ATTO 550 | 553 | 576 |
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ATTO 565 | 563 | 592 |
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ATTO 633 | 629 | 658 |
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ATTO 635 | 635 | 658 |
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ATTO 647 | 644 | 670 |
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ATTO 647N | 645 | 668 |
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ATTO 655 | 663 | 683 |
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ATTO 680 | 680 | 700 |
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ATTO 700 | 699 | 719 |
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BD Horizon V450 | 406 | 449 |
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BFP (EBFP) | 380 | 440 |
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BOBO™-1 | 461 | 484 |
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BOBO™-3 | 570 | 605 |
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BODIPY 650/665-X | 646 | 664 |
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BODIPY FL | 505 | 512 |
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BODIPY FL-MeOH | 502 | 511 |
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BODIPY TMR-X | 544 | 573 |
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BODIPY TR-X (MeOH) | 544 | 573 |
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Calcein | 494 | 514 |
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Calcium Crimson | 589 | 609 |
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Calcium Orange | 589 | 609 |
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Carboxynaphthofluorescein | 599 | 674 |
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Cascade Blue™ | 401 | 419 |
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CellTrace calcein violet | 400 | 452 |
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CellTracker Red CMTPX | 586 | 614 |
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CFP2 | 490 | 510 |
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Cy2™ | 492 | 507 |
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Cy3.5™ | 578 | 591 |
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Cy3™ | 554 | 566 |
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Cy5.5™ | 672 | 690 |
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Cy5™ | 649 | 666 |
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CyQUANT GR-DNA | 502 | 523 |
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DAF-FM-NO | 495 | 519 |
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DAPI | 359 | 461 |
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DDAO | 648 | 657 |
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Dendra2 (Green) | 491 | 507 |
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Dendra2 (Red) | 554 | 569 |
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DiD (DiIC18(5)) - Lipophilic Tracer | 648 | 670 |
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DiI | 551 | 569 |
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DiO | 475 | 500 |
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Dronpa | 502 | 516 |
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DsRed | 559 | 583 |
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DsRed-Express | 556 | 584 |
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dTomato | 556 | 582 |
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DY-505-Phalloidin | 503 | 530 |
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DY-590 | 582 | 599 |
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DyLight 405 | 398 | 420 |
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DyLight 649 | 651 | 673 |
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DyLight 680 | 676 | 705 |
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ecliptic pHluorin pH5.5 | 473 | 507 |
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Emerald | 491 | 511 |
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Ethidium bromide | 518 | 603 |
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Ethidium homodimer | 527 | 617 |
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evoglow-Bs1 | 449 | 496 |
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FITC (Fluorescein) | 495 | 519 |
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Fluo-4 | 494 | 516 |
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Fluorescein dextran | 501 | 524 |
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Fluorescein-pH 8.0 | 489 | 517 |
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Fluoro-Emerald | 494 | 518 |
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FluoSpheres Dark Red fluorescent microspheres | 656 | 683 |
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FluoSpheres Red fluorescent microspheres | 577 | 607 |
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FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
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GFP (EGFP) | 489 | 511 |
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Gold 550 | 551 | 564 |
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Green 496 | 496 | 520 |
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Green 500 | 501 | 524 |
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HCS CellMask Red cytoplasmic | 622 | 644 |
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HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
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HCS LipidTOX Red neutral lipid stain | 582 | 616 |
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HCS LipidTOX Red phospholipidosis | 584 | 608 |
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HiLyte Fluor™ 405 | 403 | 428 |
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HiLyte Fluor™ 488 | 497 | 526 |
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HiLyte Fluor™ 555 | 550 | 566 |
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HiLyte Fluor™ 647 | 649 | 673 |
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KFP-Red | 575 | 599 |
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LDS 751 | 560 | 711 |
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LIVE-DEAD Fixable Far Red Dead Cell Stain | 653 | 668 |
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LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
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LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
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LOLO-1 | 568 | 580 |
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LysoSensor Blue | 374 | 424 |
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LysoSensor Green | 448 | 502 |
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LysoTracker Green | 501 | 509 |
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LysoTracker Red | 573 | 592 |
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LysoTracker Yellow HCK-123 | 488 | 565 |
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Magnesium Orange | 550 | 574 |
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mApple | 566 | 594 |
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Marina Blue | 363 | 461 |
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mCherry | 587 | 610 |
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Merocyanine 540 | 559 | 579 |
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mHoneyDew | 478 | 562 |
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MitoTracker™ Deep Red | 641 | 662 |
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MitoTracker™ Green | 490 | 512 |
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MitoTracker™ Orange | 551 | 575 |
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MitoTracker™ Red | 578 | 598 |
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mOrange | 546 | 562 |
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mRFP | 555 | 583 |
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mRFP1 | 584 | 700 |
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mRuby | 558 | 605 |
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mStrawberry | 574 | 596 |
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mTangerine | 568 | 585 |
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mTFP1 | 462 | 492 |
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mWasabi | 493 | 509 |
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NBD-X (MeOH) | 467 | 538 |
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NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
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Nile blue-EtOH | 631 | 660 |
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Nile red-triglyceride | 510 | 583 |
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OFP | 547 | 567 |
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Oregon Green™ 488 | 498 | 526 |
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Pacific Blue™ | 404 | 455 |
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pHrodo™, succinimidyl ester | 560 | 587 |
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PicoGreen® | 502 | 522 |
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PKH67 | 488 | 500 |
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POPO-3 | 533 | 574 |
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PO-PRO-3 | 539 | 570 |
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Propidium Iodide (PI) | 305 | 617 |
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Pro-Q Diamond | 556 | 583 |
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Qdot® 525 Nanocrystals | 300 | 525 |
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Qdot® 585 Nanocrystals | 300 | 588 |
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Qdot® 605 Nanocrystals | 300 | 602 |
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Qdot® 705 Nanocrystals | 300 | 702 |
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Qdot® 800 Nanocrystals | 300 | 792 |
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Quasar® 705 | 688 | 706 |
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ReAsH-CCPGCC | 592 | 606 |
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Red 580 | 580 | 603 |
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Red 650 | 649 | 665 |
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Resorufin | 571 | 584 |
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Rhod-2 | 553 | 577 |
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Rhodamine 110 | 497 | 519 |
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Rhodamine Green | 497 | 523 |
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Rhodamine phalloidin | 558 | 575 |
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Rhodamine Red-X | 573 | 591 |
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Rhodol Green | 497 | 524 |
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R-phycoerythrin (PE) | 565 | 576 |
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sgBFP™ | 387 | 451 |
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sgGFP™ (super glow GFP) | 472 | 506 |
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SNARF-1 488nm (ph 6.0) | 548 | 586 |
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SNARF-1 488nm (ph 9.0) | 576 | 638 |
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SNARF-1 514nm (ph 6.0) | 549 | 587 |
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Sodium Green | 507 | 532 |
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SpectrumBlue | 405 | 449 |
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SpectrumFRed (Far Red) | 650 | 673 |
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SpectrumGreen | 497 | 538 |
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SpectrumOrange | 554 | 587 |
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Sulforhodamine 101-EtOH | 578 | 593 |
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SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
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SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
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SYBR Safe DNA gel stain-DNA | 509 | 526 |
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SYTO 11 | 506 | 525 |
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SYTO 13 | 448 | 506 |
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SYTO 16 | 489 | 520 |
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SYTO 59 | 622 | 644 |
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SYTO 60 | 650 | 681 |
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SYTO 61 | 618 | 650 |
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SYTO 82 | 540 | 559 |
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SYTO 9 | 483 | 500 |
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SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
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SYTOX Green-DNA | 504 | 524 |
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SYTOX Orange | 547 | 570 |
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SYTOX Red-DNA | 640 | 658 |
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TagBFP | 402 | 457 |
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TagCFP | 458 | 480 |
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Tetramethylrhodamine dextran | 554 | 582 |
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Texas Red DHPE | 584 | 608 |
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TO-PRO-3 | 642 | 657 |
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TOTO-1 | 514 | 531 |
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TOTO-3 | 642 | 661 |
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TRITC (Tetramethylrhodamine) | 552 | 578 |
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TurboGFP | 482 | 503 |
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TurboRFP | 553 | 573 |
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Vybrant DyeCycle Violet | 369 | 437 |
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wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
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X-Rhod-1 Indicator | 580 | 601 |
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YO-PRO-1 | 491 | 506 |
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YOYO-1 | 491 | 508 |
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