This Full-multiband quad-band set is designed for imaging DAPI, FITC, TRITC, and Cy5 (and other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in quad-band performance, and has been optimized for popular LED-based light engines including 365 nm DAPI LEDs. This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a quad-band beamsplitter, emitter, and exciter filter, all with "no burn-out" performance.
Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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2,438.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-378/474/554/635-25
FL-004441 / 0
- Tavg > 85% 352 – 404 nm, Tavg > 93% 461 – 487.5 nm, Tavg > 93% 543 – 566 nm, Tavg > 93% 626 – 644 nm
- Center Wavelengths of 378 nm, 474.3 nm, 554.5 nm & 635 nm and GMBWs of 52 nm, 26.5 nm, 23 nm & 18 nm
FF01-432/515/595/730-25
FL-004669 / 0
- Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 530 nm, Tavg > 93% 580 – 611 nm & Tavg > 93% 661 – 800 nm
- Center Wavelengths of 432 nm, 515 nm, 595 nm & 730 nm and GMBWs of 36 nm, 30 nm, 31 nm & 139 nm
FF409/493/573/652-Di02-25x36
FL-007110 / 0
- Dichroic Beamsplitter with edges at 409 nm, 493 nm, 573 nm & 652 nm
- Ravg > 95% 350 – 404 nm, Ravg > 95% 461 – 487.5 nm, Ravg > 95% 543 – 566 nm & Ravg > 95% 626 – 644 nm
- Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 530 nm, Tavg > 93% 580 – 611 nm & Tavg > 93% 659.5 – 850 nm
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
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| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
|
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| Acridine Orange (+DNA) | 502 | 526 |
|
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| Acridine Yellow | 470 | 550 |
|
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| Alexa Fluor® 405 | 401 | 422 |
|
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| Alexa Fluor® 430 | 431 | 541 |
|
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| Alexa Fluor® 488 | 499 | 520 |
|
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| Alexa Fluor® 500 | 503 | 525 |
|
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| Alexa Fluor® 546 | 556 | 572 |
|
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| Alexa Fluor® 555 | 553 | 565 |
|
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| Alexa Fluor® 568 | 579 | 603 |
|
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| Alexa Fluor® 633 | 631 | 647 |
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| Alexa Fluor® 647 | 653 | 668 |
|
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| Alexa Fluor® 660 | 663 | 691 |
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| Alexa Fluor® 680 | 679 | 702 |
|
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| Alexa Fluor® 700 | 696 | 719 |
|
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| AmCyan1 | 458 | 489 |
|
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| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
|
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| APC | 651 | 660 |
|
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| APC (Allophycocyanin) | 651 | 660 |
|
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| AsRed 2 | 576 | 592 |
|
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| ATTO 465 | 453 | 507 |
|
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| ATTO 488 | 500 | 525 |
|
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| ATTO 495 | 495 | 527 |
|
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| ATTO 550 | 553 | 576 |
|
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| ATTO 565 | 563 | 592 |
|
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| ATTO 633 | 629 | 658 |
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| ATTO 635 | 635 | 658 |
|
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| ATTO 647 | 644 | 670 |
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| ATTO 647N | 645 | 668 |
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| ATTO 655 | 663 | 683 |
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| ATTO 680 | 680 | 700 |
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| ATTO 700 | 699 | 719 |
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| BD Horizon V450 | 406 | 449 |
|
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| BFP (EBFP) | 380 | 440 |
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| BOBO™-1 | 461 | 484 |
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| BOBO™-3 | 570 | 605 |
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| BODIPY 650/665-X | 646 | 664 |
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| BODIPY FL | 505 | 512 |
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| BODIPY FL-MeOH | 502 | 511 |
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| BODIPY TMR-X | 544 | 573 |
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| BODIPY TR-X (MeOH) | 544 | 573 |
|
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| Calcein | 494 | 514 |
|
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| Calcium Crimson | 589 | 609 |
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| Calcium Orange | 589 | 609 |
|
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| Carboxynaphthofluorescein | 599 | 674 |
|
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| Cascade Blue™ | 401 | 419 |
|
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| CellTrace calcein violet | 400 | 452 |
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| CellTracker Red CMTPX | 586 | 614 |
|
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| CFP2 | 490 | 510 |
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| Cy2™ | 492 | 507 |
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| Cy3.5™ | 578 | 591 |
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| Cy3™ | 554 | 566 |
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| Cy5.5™ | 672 | 690 |
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| Cy5™ | 649 | 666 |
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| CyQUANT GR-DNA | 502 | 523 |
|
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| DAF-FM-NO | 495 | 519 |
|
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| DAPI | 359 | 461 |
|
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| DDAO | 648 | 657 |
|
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| Dendra2 (Green) | 491 | 507 |
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| Dendra2 (Red) | 554 | 569 |
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| DiD (DiIC18(5)) - Lipophilic Tracer | 648 | 670 |
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| DiI | 551 | 569 |
|
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| DiO | 475 | 500 |
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| Dronpa | 502 | 516 |
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| DsRed | 559 | 583 |
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| DsRed-Express | 556 | 584 |
|
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| dTomato | 556 | 582 |
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| DY-505-Phalloidin | 503 | 530 |
|
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| DY-590 | 582 | 599 |
|
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| DyLight 405 | 398 | 420 |
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| DyLight 649 | 651 | 673 |
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| DyLight 680 | 676 | 705 |
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| ecliptic pHluorin pH5.5 | 473 | 507 |
|
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| Emerald | 491 | 511 |
|
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| Ethidium bromide | 518 | 603 |
|
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| Ethidium homodimer | 527 | 617 |
|
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| evoglow-Bs1 | 449 | 496 |
|
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| FITC (Fluorescein) | 495 | 519 |
|
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| Fluo-4 | 494 | 516 |
|
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| Fluorescein dextran | 501 | 524 |
|
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| Fluorescein-pH 8.0 | 489 | 517 |
|
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| Fluoro-Emerald | 494 | 518 |
|
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| FluoSpheres Dark Red fluorescent microspheres | 656 | 683 |
|
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| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
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| GFP (EGFP) | 489 | 511 |
|
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| Gold 550 | 551 | 564 |
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| Green 496 | 496 | 520 |
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| Green 500 | 501 | 524 |
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| HCS CellMask Red cytoplasmic | 622 | 644 |
|
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| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
|
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
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| HiLyte Fluor™ 405 | 403 | 428 |
|
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| HiLyte Fluor™ 488 | 497 | 526 |
|
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| HiLyte Fluor™ 555 | 550 | 566 |
|
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| HiLyte Fluor™ 647 | 649 | 673 |
|
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| KFP-Red | 575 | 599 |
|
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| LDS 751 | 560 | 711 |
|
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| LIVE-DEAD Fixable Far Red Dead Cell Stain | 653 | 668 |
|
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
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| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
|
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| LOLO-1 | 568 | 580 |
|
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| LysoSensor Blue | 374 | 424 |
|
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| LysoSensor Green | 448 | 502 |
|
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| LysoTracker Green | 501 | 509 |
|
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| LysoTracker Red | 573 | 592 |
|
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| LysoTracker Yellow HCK-123 | 488 | 565 |
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| Magnesium Orange | 550 | 574 |
|
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| mApple | 566 | 594 |
|
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| Marina Blue | 363 | 461 |
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| mCherry | 587 | 610 |
|
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| Merocyanine 540 | 559 | 579 |
|
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| mHoneyDew | 478 | 562 |
|
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| MitoTracker™ Deep Red | 641 | 662 |
|
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| MitoTracker™ Green | 490 | 512 |
|
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| MitoTracker™ Orange | 551 | 575 |
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| MitoTracker™ Red | 578 | 598 |
|
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| mOrange | 546 | 562 |
|
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| mRFP | 555 | 583 |
|
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| mRFP1 | 584 | 700 |
|
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| mRuby | 558 | 605 |
|
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| mStrawberry | 574 | 596 |
|
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| mTangerine | 568 | 585 |
|
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| mTFP1 | 462 | 492 |
|
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| mWasabi | 493 | 509 |
|
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| NBD-X (MeOH) | 467 | 538 |
|
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
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| Nile blue-EtOH | 631 | 660 |
|
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| Nile red-triglyceride | 510 | 583 |
|
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| OFP | 547 | 567 |
|
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| Oregon Green™ 488 | 498 | 526 |
|
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| Pacific Blue™ | 404 | 455 |
|
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| pHrodo™, succinimidyl ester | 560 | 587 |
|
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| PicoGreen® | 502 | 522 |
|
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| PKH67 | 488 | 500 |
|
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| POPO-3 | 533 | 574 |
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| PO-PRO-3 | 539 | 570 |
|
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| Propidium Iodide (PI) | 305 | 617 |
|
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| Pro-Q Diamond | 556 | 583 |
|
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| Qdot® 525 Nanocrystals | 300 | 525 |
|
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| Qdot® 585 Nanocrystals | 300 | 588 |
|
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| Qdot® 605 Nanocrystals | 300 | 602 |
|
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| Qdot® 705 Nanocrystals | 300 | 702 |
|
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| Qdot® 800 Nanocrystals | 300 | 792 |
|
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| Quasar® 705 | 688 | 706 |
|
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| ReAsH-CCPGCC | 592 | 606 |
|
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| Red 580 | 580 | 603 |
|
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| Red 650 | 649 | 665 |
|
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| Resorufin | 571 | 584 |
|
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| Rhod-2 | 553 | 577 |
|
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| Rhodamine 110 | 497 | 519 |
|
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| Rhodamine Green | 497 | 523 |
|
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| Rhodamine phalloidin | 558 | 575 |
|
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| Rhodamine Red-X | 573 | 591 |
|
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| Rhodol Green | 497 | 524 |
|
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| R-phycoerythrin (PE) | 565 | 576 |
|
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| sgBFP™ | 387 | 451 |
|
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| sgGFP™ (super glow GFP) | 472 | 506 |
|
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| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
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| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
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| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
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| Sodium Green | 507 | 532 |
|
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| SpectrumBlue | 405 | 449 |
|
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| SpectrumFRed (Far Red) | 650 | 673 |
|
|
| SpectrumGreen | 497 | 538 |
|
|
| SpectrumOrange | 554 | 587 |
|
|
| Sulforhodamine 101-EtOH | 578 | 593 |
|
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
|
| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
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| SYTO 11 | 506 | 525 |
|
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| SYTO 13 | 448 | 506 |
|
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| SYTO 16 | 489 | 520 |
|
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| SYTO 59 | 622 | 644 |
|
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| SYTO 60 | 650 | 681 |
|
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| SYTO 61 | 618 | 650 |
|
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| SYTO 82 | 540 | 559 |
|
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| SYTO 9 | 483 | 500 |
|
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
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| SYTOX Green-DNA | 504 | 524 |
|
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| SYTOX Orange | 547 | 570 |
|
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| SYTOX Red-DNA | 640 | 658 |
|
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| TagBFP | 402 | 457 |
|
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| TagCFP | 458 | 480 |
|
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| Tetramethylrhodamine dextran | 554 | 582 |
|
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| Texas Red DHPE | 584 | 608 |
|
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| TO-PRO-3 | 642 | 657 |
|
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| TOTO-1 | 514 | 531 |
|
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| TOTO-3 | 642 | 661 |
|
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| TRITC (Tetramethylrhodamine) | 552 | 578 |
|
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| TurboGFP | 482 | 503 |
|
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| TurboRFP | 553 | 573 |
|
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| Vybrant DyeCycle Violet | 369 | 437 |
|
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| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
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| X-Rhod-1 Indicator | 580 | 601 |
|
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| YO-PRO-1 | 491 | 506 |
|
|
| YOYO-1 | 491 | 508 |
|
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