Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.
This 6-filter "Pinkel" quad-band set is designed for imaging of a sample that is simultaneously labeled with DAPI, FITC, TRITC, and Cy5 (or other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines, including 365 nm DAPI LEDs, compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a quad-band beamsplitter and a quad-band emitter (these can be offered assembled in a microscope cube), plus four "no burn-out" single-band exciters that are intended to be mounted in the LED-based light engines, or in an exciter filter wheel or slider.
Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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3,348.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-378/52-25
FL-004583 / 0
- Tavg > 85% 352 – 404 nm
- Center Wavelength of 378 nm and GMBW of 52 nm
- FWHM of 54.6 nm
FF01-474/27-25
FL-004596 / 0
- Tavg > 93% 461.0 – 487.5 nm
- Center Wavelength of 474.3 nm and GMBW of 26.5 nm
- FWHM of 30.9 nm
FF01-554/23-25
FL-004612 / 0
- Tavg > 93% 543.0 – 566.0 nm
- Center Wavelength of 554.5 nm and GMBW of 23.0 nm
- FWHM of 28.1 nm
FF01-432/515/595/730-25
FL-004669 / 0
- Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 530 nm, Tavg > 93% 580 – 611 nm & Tavg > 93% 661 – 800 nm
- Center Wavelengths of 432 nm, 515 nm, 595 nm & 730 nm and GMBWs of 36 nm, 30 nm, 31 nm & 139 nm
FF01-635/18-25
FL-004696 / 0
- Tavg > 93% 626 – 644 nm
- Center Wavelength of 635 nm and GMBW of 18 nm
- FWHM of 23.9 nm
FF409/493/573/652-Di02-25x36
FL-007110 / 0
- Dichroic Beamsplitter with edges at 409 nm, 493 nm, 573 nm & 652 nm
- Ravg > 95% 350 – 404 nm, Ravg > 95% 461 – 487.5 nm, Ravg > 95% 543 – 566 nm & Ravg > 95% 626 – 644 nm
- Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 530 nm, Tavg > 93% 580 – 611 nm & Tavg > 93% 659.5 – 850 nm
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This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
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| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Acridine Yellow | 470 | 550 |
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| Alexa Fluor® 405 | 401 | 422 |
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| Alexa Fluor® 430 | 431 | 541 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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| Alexa Fluor® 546 | 556 | 572 |
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| Alexa Fluor® 555 | 553 | 565 |
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| Alexa Fluor® 568 | 579 | 603 |
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| Alexa Fluor® 633 | 631 | 647 |
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| Alexa Fluor® 647 | 653 | 668 |
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| Alexa Fluor® 660 | 663 | 691 |
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| Alexa Fluor® 680 | 679 | 702 |
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| Alexa Fluor® 700 | 696 | 719 |
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| AmCyan1 | 458 | 489 |
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| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
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| APC | 651 | 660 |
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| APC (Allophycocyanin) | 651 | 660 |
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| AsRed 2 | 576 | 592 |
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| ATTO 465 | 453 | 507 |
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| ATTO 488 | 500 | 525 |
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| ATTO 495 | 495 | 527 |
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| ATTO 550 | 553 | 576 |
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| ATTO 565 | 563 | 592 |
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| ATTO 633 | 629 | 658 |
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| ATTO 635 | 635 | 658 |
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| ATTO 647 | 644 | 670 |
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| ATTO 647N | 645 | 668 |
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| ATTO 655 | 663 | 683 |
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| ATTO 680 | 680 | 700 |
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| ATTO 700 | 699 | 719 |
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| BD Horizon V450 | 406 | 449 |
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| BFP (EBFP) | 380 | 440 |
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| BOBO™-1 | 461 | 484 |
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| BOBO™-3 | 570 | 605 |
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| BODIPY 650/665-X | 646 | 664 |
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| BODIPY FL | 505 | 512 |
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| BODIPY FL-MeOH | 502 | 511 |
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| BODIPY TMR-X | 544 | 573 |
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| BODIPY TR-X (MeOH) | 544 | 573 |
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| Calcein | 494 | 514 |
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| Calcium Crimson | 589 | 609 |
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| Calcium Orange | 589 | 609 |
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| Carboxynaphthofluorescein | 599 | 674 |
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| Cascade Blue™ | 401 | 419 |
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| CellTrace calcein violet | 400 | 452 |
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| CellTracker Red CMTPX | 586 | 614 |
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| CFP2 | 490 | 510 |
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| Cy2™ | 492 | 507 |
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| Cy3.5™ | 578 | 591 |
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| Cy3™ | 554 | 566 |
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| Cy5.5™ | 672 | 690 |
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| Cy5™ | 649 | 666 |
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| CyQUANT GR-DNA | 502 | 523 |
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| DAF-FM-NO | 495 | 519 |
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| DAPI | 359 | 461 |
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| DDAO | 648 | 657 |
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| Dendra2 (Green) | 491 | 507 |
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| Dendra2 (Red) | 554 | 569 |
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| DiD (DiIC18(5)) - Lipophilic Tracer | 648 | 670 |
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