BrightLine® Pinkel filter set, optimized for DAPI, FITC, TRITC, & Cy5 and other like fluorophores, illuminated with LED-based light engines

Semrock Part Number: LED-DA/FI/TR/Cy5-4X-B-LDMK-ZERO

IDEX Part Number: S-001109  /

Semrock

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This 6-filter "Pinkel" quad-band set is designed for imaging of a sample that is simultaneously labeled with DAPI, FITC, TRITC, and Cy5 (or other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines, including 365 nm DAPI LEDs, compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a quad-band beamsplitter and a quad-band emitter (these can be offered assembled in a microscope cube), plus four "no burn-out" single-band exciters that are intended to be mounted in the LED-based light engines, or in an exciter filter wheel or slider.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

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  • Price Not Available.
  • 3,278.00 USD Each
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FF01-378/52-25

FL-004583 / 0

Out of Stock Backordered In Stock
  • Tavg > 85% 352 – 404 nm
  • Center Wavelength of 378 nm and GMBW of 52 nm
  • FWHM of 54.6 nm

FF01-474/27-25

FL-004596 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 461.0 – 487.5 nm
  • Center Wavelength of 474.3 nm and GMBW of 26.5 nm
  • FWHM of 30.9 nm

FF01-554/23-25

FL-004612 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 543.0 – 566.0 nm
  • Center Wavelength of 554.5 nm and GMBW of 23.0 nm
  • FWHM of 28.1 nm

FF01-432/515/595/730-25

FL-004669 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 530 nm, Tavg > 93% 580 – 611 nm & Tavg > 93% 661 – 800 nm
  • Center Wavelengths of 432 nm, 515 nm, 595 nm & 730 nm and GMBWs of 36 nm, 30 nm, 31 nm & 139 nm

FF01-635/18-25

FL-004696 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 626 – 644 nm
  • Center Wavelength of 635 nm and GMBW of 18 nm
  • FWHM of 23.9 nm

FF409/493/573/652-Di02-25x36

FL-007110 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edges at 409 nm, 493 nm, 573 nm & 652 nm
  • Ravg > 95% 350 – 404 nm, Ravg > 95% 461 – 487.5 nm, Ravg > 95% 543 – 566 nm & Ravg > 95% 626 – 644 nm
  • Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 530 nm, Tavg > 93% 580 – 611 nm & Tavg > 93% 659.5 – 850 nm

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This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)21.8 mm5.0 mm
Emitter (Diameter)23.3 mm5.0 mm
Dichroic (L × W)22.0 mm × 29.0 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-carboxynapthofluorescein (pH 10) 555 615
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
Acridine Orange (+DNA) 502 526
Acridine Yellow 470 550
Alexa Fluor® 405 401 422
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 546 556 572
Alexa Fluor® 555 553 565
Alexa Fluor® 568 579 603
Alexa Fluor® 633 631 647
Alexa Fluor® 647 653 668
Alexa Fluor® 660 663 691
Alexa Fluor® 680 679 702
Alexa Fluor® 700 696 719
AmCyan1 458 489
Amplex UltraRed peroxidation product (pH 7.5) 568 581
APC 651 660
APC (Allophycocyanin) 651 660
AsRed 2 576 592
ATTO 465 453 507
ATTO 488 500 525
ATTO 495 495 527
ATTO 550 553 576
ATTO 565 563 592
ATTO 633 629 658
ATTO 635 635 658
ATTO 647 644 670
ATTO 647N 645 668
ATTO 655 663 683
ATTO 680 680 700
ATTO 700 699 719
BD Horizon V450 406 449
BFP (EBFP) 380 440
BOBO™-1 461 484
BOBO™-3 570 605
BODIPY 650/665-X 646 664
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
BODIPY TMR-X 544 573
BODIPY TR-X (MeOH) 544 573
Calcein 494 514
Calcium Crimson 589 609
Calcium Orange 589 609
Carboxynaphthofluorescein 599 674
Cascade Blue™ 401 419
CellTrace calcein violet 400 452
CellTracker Red CMTPX 586 614
CFP2 490 510
Cy2™ 492 507
Cy3.5™ 578 591
Cy3™ 554 566
Cy5.5™ 672 690
Cy5™ 649 666
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
DAPI 359 461
DDAO 648 657
Dendra2 (Green) 491 507
Dendra2 (Red) 554 569
DiD (DiIC18(5)) - Lipophilic Tracer 648 670