BrightLine® Sedat filter set, optimized for DAPI, FITC, TRITC, & Cy5 and other like fluorophores, illuminated with LED-based light engines

Semrock Part Number: LED-DA/FI/TR/Cy5-4X4M-B-000

IDEX Part Number: S-000831  /

Semrock

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This 9-filter "Sedat" quad-band set is designed for high-speed, sequential imaging of DAPI, FITC, TRITC, and Cy5 (and other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a quad-band beamsplitter with four single-band emitters intended to be mounted in filter wheels and four single-band exciters which can be installed into the LED light engine or mounted in filter wheels.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight spectral plotting & analysis tool.

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  • Price Not Available.
  • 3,640.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Filter set mounted in microscope holders/cubes found below in related products section.
Set Components

FF01-378/52-25

FL-004583

Each
  • Tavg > 85% 352 – 404 nm
  • Center Wavelength of 378 nm and GMBW of 52 nm
  • FWHM of 54.6 nm

FL-004583

FF01-432/36-25

FL-004373

Each
  • Tavg > 93% 414 – 450 nm
  • Center Wavelength of 432 nm and GMBW of 36 nm
  • FWHM of 39.6 nm

FL-004373

FF01-474/27-25

FL-004596

Each
  • Tavg > 93% 461.0 – 487.5 nm
  • Center Wavelength of 474.3 nm and GMBW of 26.5 nm
  • FWHM of 30.9 nm

FL-004596

FF01-515/30-25

FL-004382

Each
  • Tavg > 93% 499.5 – 530 nm
  • Center Wavelength of 514.8 nm and GMBW of 30.5 nm
  • FWHM of 35 nm

FL-004382

FF01-554/23-25

FL-004612

Each
  • Tavg > 93% 543.0 – 566.0 nm
  • Center Wavelength of 554.5 nm and GMBW of 23.0 nm
  • FWHM of 28.1 nm

FL-004612

FF01-595/31-25

FL-004391

Each
  • Tavg > 93% 580 – 611 nm
  • Center Wavelength of 595.5 nm and GMBW of 31 nm
  • FWHM of 36.7 nm

FL-004391

FF01-635/18-25

FL-004696

Each
  • Tavg > 93% 626 – 644 nm
  • Center Wavelength of 635 nm and GMBW of 18 nm
  • FWHM of 23.9 nm

FL-004696

FF01-698/70-25

FL-004704

Each
  • Tavg > 93% 662.5 - 732.5 nm
  • Center Wavelength of 698 nm and GMBW of 70 nm
  • FWHM of 76.9 nm

FL-004704

FF409/493/573/652-Di02-25x36

FL-007110

Each
  • Dichroic Beamsplitter with edges at 409 nm, 493 nm, 573 nm & 652 nm
  • Ravg > 95% 350 – 404 nm, Ravg > 95% 461 – 487.5 nm, Ravg > 95% 543 – 566 nm & Ravg > 95% 626 – 644 nm
  • Tavg > 93% 414 – 450 nm, Tavg > 93% 499.5 – 530 nm, Tavg > 93% 580 – 611 nm & Tavg > 93% 659.5 – 850 nm

FL-007110

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

LED-DA/FI/TR/Cy5-B-000

S-000841 / 0

Out of Stock Backordered In Stock
Multiband set with quad-band exciter, emitter, and beamsplitter filters. Ideal for use with: Blue: DAPI; Green: FITC , GFP ; Orange: TRITC ; Red: Cy5

LED-DA/FI/TR/Cy5-4X-B-000

S-000836 / 0

Out of Stock Backordered In Stock
6-filter "Pinkel" quad-band set with 4 exciters and quad-band emitter and beamsplitter filters. Ideal for use with: Blue: DAPI; Green: FITC , GFP ; Orange: TRITC ; Red: Cy5

LED-DA/FI/TR/Cy5-4X4M-B-OMF

S-002820 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

LED-DA/FI/TR/Cy5-4X4M-B-OFF

S-002818 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

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TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
2-dodecylresorufin-lipid 582 595
5-carboxynapthofluorescein (pH 10) 555 615
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
7-amino-4-methylcoumarin (AMC) 346 442
7-hydroxy-4-methylcoumarin 360 442
Acridine Orange (+DNA) 502 526
Acridine Yellow 470 550
Alexa Fluor® 350 343 441
Alexa Fluor® 405 401 422
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 546 556 572
Alexa Fluor® 555 553 565
Alexa Fluor® 568 579 603
Alexa Fluor® 633 631 647
Alexa Fluor® 647 653 668
Alexa Fluor® 660 663 691
Alexa Fluor® 680 679 702
Alexa Fluor® 700 696 719
AMCA (Aminomethylcoumarin) 350 448
AmCyan1 458 489
Amplex UltraRed peroxidation product (pH 7.5) 568 581
APC 651 660
APC (Allophycocyanin) 651 660
AsRed 2 576 592
ATTO 465 453 507
ATTO 488 500 525
ATTO 495 495 527
ATTO 550 553 576
ATTO 565 563 592
ATTO 633 629 658
ATTO 635 635 658
ATTO 647 644 670
ATTO 647N 645 668
ATTO 655 663 683
ATTO 680 680 700
ATTO 700 699 719
BD Horizon V450 406 449
BFP (EBFP) 380 440
BOBO™-1 461 484
BOBO™-3 570 605
BODIPY 650/665-X 646 664
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
BODIPY TMR-X 544 573
BODIPY TR-X (MeOH) 544 573
BUV395 - BD Horizon Brilliant™ Ultraviolet 395 348 395
Calcein 494 514
Calcium Crimson 589 609
Calcium Orange 589 609
Calcofluor White 351 444
Carboxynaphthofluorescein 599 674
Cascade Blue™ 401 419
CellTrace calcein violet 400 452
CellTracker Red CMTPX 586 614
CFP2 490 510
Cy2™ 492 507
Cy3.5™ 578 591
Cy3™ 554 566
Cy5.5™ 672 690
Cy5™ 649 666
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
DAPI 359 461
DDAO 648 657
Dendra2 (Green) 491 507
Dendra2 (Red) 554 569
DiD (DiIC18(5)) - Lipophilic Tracer 648 670
DiFMU-pH 9.0 358 450
DiI 551 569
DiO 475 500
Dronpa 502 516
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-505-Phalloidin 503 530
DY-590 582 599
DY-630 634 656
DY-675 675 699
DyLight 350 351 434
DyLight 405 398 420
DyLight 549 553 569
DyLight 649 651 673
DyLight 680 676 705
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
Ethidium bromide 518 603
Ethidium homodimer 527 617
evoglow-Bs1 449 496
FITC (Fluorescein) 495 519
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
Fluoro-Gold™ 365 418
FluoSpheres Blue fluorescent microspheres 356 414
FluoSpheres Dark Red fluorescent microspheres 656 683
FluoSpheres Red fluorescent microspheres 577 607
FluoSpheres Yellow-Green fluorescent microspheres 503 514
GFP (EGFP) 489 511
Gold 550 551 564
Green 496 496 520
Green 500 501 524
HCS CellMask Red cytoplasmic 622 644
HCS LipidTOX Deep Red neutral lipid stain 635 652
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608