This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring Venus (or other key fluorophores – see complete list above). This state-of-the-art set has been optimized for popular LED based light sources. This set has been designed to deliver significant fluorescence signal improvements for LED based light sources compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps.
Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,464.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF02-809/81-25
FL-004520 / 0
- Tavg > 93% 768.5 – 849.5 nm
- Center Wavelength of 809 nm and GMBW of 81 nm
- FWHM of 88.4 nm
FF01-735/28-25
FL-004707 / 0
- Tavg > 93% 721 – 749 nm
- Center Wavelength of 735 nm and GMBW of 28 nm
- FWHM of 33.1 nm
FF757-Di01-25x36
FL-007068 / 0
- Dichroic Beamsplitter with edge at 757 nm
- Ravg > 98% 450 – 746 nm
- Tavg > 93% 768 – 1100 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| Alexa Fluor® 750 | 751 | 776 |
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| Alexa Fluor® 790 | 782 | 805 |
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| ATTO 725 | 727 | 754 |
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| ATTO 740 | 740 | 764 |
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| Cy7™ | 753 | 775 |
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|
| DiR | 750 | 782 |
|
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| DY-781 | 784 | 802 |
|
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| DyLight 750 | 751 | 778 |
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| DyLight 800 | 777 | 794 |
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| HiLyte Fluor™ 750 | 750 | 778 |
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| IRDye 800 CW-MeOH | 778 | 801 |
|
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| IRDye 800CW | 775 | 792 |
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| IRDye 800CW-NHS Ester | 775 | 792 |
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| IRDye 800RS | 787 | 812 |
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| LIVE-DEAD Fixable Near-IR Dead Cell Stain | 752 | 776 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF02-809/81-25
FL-004520 / 0
- Tavg > 93% 768.5 – 849.5 nm
- Center Wavelength of 809 nm and GMBW of 81 nm
- FWHM of 88.4 nm
FF01-735/28-25
FL-004707 / 0
- Tavg > 93% 721 – 749 nm
- Center Wavelength of 735 nm and GMBW of 28 nm
- FWHM of 33.1 nm
FF757-Di01-25x36
FL-007068 / 0
- Dichroic Beamsplitter with edge at 757 nm
- Ravg > 98% 450 – 746 nm
- Tavg > 93% 768 – 1100 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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LPD02-532RU-25
FL-008055