Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.
This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring Cy5 (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market. Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,429.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-635/18-25
FL-004696 / 0
- Tavg > 93% 626 – 644 nm
- Center Wavelength of 635 nm and GMBW of 18 nm
- FWHM of 23.9 nm
FF01-680/42-25
FL-004700 / 0
- Tavg > 93% 659.5 – 701 nm
- Center Wavelength of 680.3 nm and GMBW of 41.5 nm
- FWHM of 47 nm
FF652-Di01-25x36
FL-007158 / 0
- Dichroic Beamsplitter with edge at 652 nm
- Ravg > 98% 350 – 644 nm
- Tavg > 93% 659.5 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
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This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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| Alexa Fluor® 633 | 631 | 647 |
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| Alexa Fluor® 635 | 633 | 648 |
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| Alexa Fluor® 647 | 653 | 668 |
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| Alexa Fluor® 660 | 663 | 691 |
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| Alexa Fluor® 680 | 679 | 702 |
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| APC | 651 | 660 |
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| APC (Allophycocyanin) | 651 | 660 |
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| ATTO 620 | 618 | 642 |
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| ATTO 633 | 629 | 658 |
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| ATTO 635 | 635 | 658 |
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| ATTO 647 | 644 | 670 |
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| ATTO 647N | 645 | 668 |
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| ATTO 655 | 663 | 683 |
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| ATTO 680 | 680 | 700 |
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| BODIPY 650/665-X | 646 | 664 |
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| Carboxynaphthofluorescein | 599 | 674 |
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| Cy5.5™ | 672 | 690 |
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| Cy5™ | 649 | 666 |
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| DDAO | 648 | 657 |
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| DiD (DiIC18(5)) - Lipophilic Tracer | 648 | 670 |
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| DyLight 649 | 651 | 673 |
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| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
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| FluoSpheres Dark Red fluorescent microspheres | 656 | 683 |
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| HCS CellMask Red cytoplasmic | 622 | 644 |
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| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
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| HiLyte Fluor™ 647 | 649 | 673 |
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| LIVE-DEAD Fixable Far Red Dead Cell Stain | 653 | 668 |
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| MitoTracker™ Deep Red | 641 | 662 |
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| Nile blue-EtOH | 631 | 660 |
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| Qdot® 655 Nanocrystals | 300 | 654 |
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| Qdot® 705 Nanocrystals | 300 | 702 |
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| Red 650 | 649 | 665 |
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| SpectrumFRed (Far Red) | 650 | 673 |
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| SYTO 59 | 622 | 644 |
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| SYTO 60 | 650 | 681 |
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| SYTO 61 | 618 | 650 |
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| SYTOX Red-DNA | 640 | 658 |
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| TO-PRO-3 | 642 | 657 |
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| TOTO-3 | 642 | 661 |
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total selected
Clear selection
Select all 3 products from all pages.
FF01-635/18-25
FL-004696 / 0
- Tavg > 93% 626 – 644 nm
- Center Wavelength of 635 nm and GMBW of 18 nm
- FWHM of 23.9 nm
FF01-680/42-25
FL-004700 / 0
- Tavg > 93% 659.5 – 701 nm
- Center Wavelength of 680.3 nm and GMBW of 41.5 nm
- FWHM of 47 nm
FF652-Di01-25x36
FL-007158 / 0
- Dichroic Beamsplitter with edge at 652 nm
- Ravg > 98% 350 – 644 nm
- Tavg > 93% 659.5 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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