Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.
This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring Cy5 (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market. Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,589.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-635/18-25
FL-004696 / 0
- Tavg > 93% 626 – 644 nm
- Center Wavelength of 635 nm and GMBW of 18 nm
- FWHM of 23.9 nm
FF01-680/42-25
FL-004700 / 0
- Tavg > 93% 659.5 – 701 nm
- Center Wavelength of 680.3 nm and GMBW of 41.5 nm
- FWHM of 47 nm
FF652-Di01-25x36
FL-007158 / 0
- Dichroic Beamsplitter with edge at 652 nm
- Ravg > 98% 350 – 644 nm
- Tavg > 93% 659.5 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
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This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.5 mm |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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| Alexa Fluor® 633 | 631 | 647 |
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| Alexa Fluor® 635 | 633 | 648 |
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| Alexa Fluor® 647 | 653 | 668 |
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| Alexa Fluor® 660 | 663 | 691 |
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| Alexa Fluor® 680 | 679 | 702 |
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| APC | 651 | 660 |
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| APC (Allophycocyanin) | 651 | 660 |
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| ATTO 620 | 618 | 642 |
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| ATTO 633 | 629 | 658 |
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| ATTO 635 | 635 | 658 |
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| ATTO 647 | 644 | 670 |
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| ATTO 647N | 645 | 668 |
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| ATTO 655 | 663 | 683 |
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| ATTO 680 | 680 | 700 |
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| BODIPY 650/665-X | 646 | 664 |
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| Carboxynaphthofluorescein | 599 | 674 |
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| Cy5.5™ | 672 | 690 |
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| Cy5™ | 649 | 666 |
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| DDAO | 648 | 657 |
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| DiD (DiIC18(5)) - Lipophilic Tracer | 648 | 670 |
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| DyLight 649 | 651 | 673 |
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| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
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| FluoSpheres Dark Red fluorescent microspheres | 656 | 683 |
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| HCS CellMask Red cytoplasmic | 622 | 644 |
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| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
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| HiLyte Fluor™ 647 | 649 | 673 |
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| LIVE-DEAD Fixable Far Red Dead Cell Stain | 653 | 668 |
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| MitoTracker™ Deep Red | 641 | 662 |
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| Nile blue-EtOH | 631 | 660 |
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| Qdot® 655 Nanocrystals | 300 | 654 |
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| Qdot® 705 Nanocrystals | 300 | 702 |
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| Red 650 | 649 | 665 |
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| SpectrumFRed (Far Red) | 650 | 673 |
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| SYTO 59 | 622 | 644 |
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| SYTO 60 | 650 | 681 |
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| SYTO 61 | 618 | 650 |
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| SYTOX Red-DNA | 640 | 658 |
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| TO-PRO-3 | 642 | 657 |
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| TOTO-3 | 642 | 661 |
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total selected
Clear selection
Select all 3 products from all pages.
FF01-635/18-25
FL-004696 / 0
- Tavg > 93% 626 – 644 nm
- Center Wavelength of 635 nm and GMBW of 18 nm
- FWHM of 23.9 nm
FF01-680/42-25
FL-004700 / 0
- Tavg > 93% 659.5 – 701 nm
- Center Wavelength of 680.3 nm and GMBW of 41.5 nm
- FWHM of 47 nm
FF652-Di01-25x36
FL-007158 / 0
- Dichroic Beamsplitter with edge at 652 nm
- Ravg > 98% 350 – 644 nm
- Tavg > 93% 659.5 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.5 mm |
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