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BrightLine® full-multiband filter set, optimized for CFP, YFP, & mCherry and other like fluorophores, illuminated with LED-based light engines

Semrock Part Number: LED-CFP/YFP/mCherry-A-000

IDEX Part Number: S-000815  /

Semrock

This Full-multiband triple-band set is designed for imaging CFP, YFP, and mCherry (and other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in triple-band performance, and has been optimized for popular LED-based light engines. This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter, emitter, and exciter filter, all with "no burn-out" performance.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,590.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
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Set Components

FF01-438/509/578-25

FL-004375

Each
  • Tavg > 93% 426 – 450 nm, Tavg > 93% 497.6 – 519.5 nm & Tavg > 93% 567.4 – 588.6 nm
  • Center Wavelengths of 438 nm, 509 nm & 578 nm and GMBWs of 24 nm, 21.9 nm & 21.2 nm

FL-004375

FF01-475/543/702-25

FL-004065

Each
  • Tavg > 93% 464 – 486 nm, Tavg > 93% 532 – 554 nm & Tavg > 93% 603 – 800 nm
  • Center Wavelengths of 475 nm, 543 nm & 701.5 nm and GMBWs of 22 nm, 22 nm & 197 nm

FL-004065

FF459/526/596-Di01-25x36

FL-007091

Each
  • Dichroic Beamsplitter with edges at 459 nm, 526 nm & 596 nm
  • Ravg > 95% 350 – 450 nm, Ravg > 95% 426 – 450 nm, Ravg > 95% 497.6 – 519.5 nm & Ravg > 95% 567.4 – 588.6 nm
  • Tavg > 93% 464 – 486 nm, Tavg > 93% 532 – 554 nm & Tavg > 93% 603 – 800 nm

FL-007091

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

LED-CFP/YFP/mCherry-A-NQF

S-001645 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

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LED-CFP/YFP/mCherry-A-NTE

S-000311 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

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LED-CFP/YFP/mCherry-A-OMF

S-001649 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

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LED-CFP/YFP/mCherry-A-ZHE

S-000313 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

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LED-CFP/YFP/mCherry-A-OFF

S-001646 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

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LED-CFP/YFP/mCherry-A-000-ZERO

S-002781 / 0

Out of Stock Backordered In Stock

Standard size -ZERO pixel set

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LED-CFP/YFP/mCherry-A-NQF-ZERO

S-000310 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

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