BrightLine® Pinkel filter set, optimized for CFP, YFP, & mCherry and other like fluorophores, illuminated with LED-based light sources

Semrock Part Number: LED-CFP/YFP/mCherry-3X-A-000

IDEX Part Number: S-001068  /

Semrock

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This 5-filter "Pinkel" triple-band set is designed for imaging of a sample that is simultaneously labeled with CFP, YFP, and mCherry, (or other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light sources compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter and a triple-band emitter (these can be offered assembled in a microscope cube), plus three "no burn-out" single-band exciters that are intended to be mounted in the LED based light source, or in an exciter filter wheel or slider.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

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  • Price Not Available.
  • 2,185.00 USD Each
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Filter set mounted in microscope holders/cubes found below in related products section.
Set Components

FF01-475/543/702-25

FL-004065

Each
  • Tavg > 93% 464 – 486 nm, Tavg > 93% 532 – 554 nm & Tavg > 93% 603 – 800 nm
  • Center Wavelengths of 475 nm, 543 nm & 701.5 nm and GMBWs of 22 nm, 22 nm & 197 nm

FL-004065

FF01-509/22-25

FL-004258

Each
  • Tavg > 93% 497.6 – 519.5 nm
  • Center Wavelength of 508.6 nm and GMBW of 21.9 nm
  • FWHM of 26.1 nm

FL-004258

FF01-578/21-25

FL-004616

Each
  • Tavg > 93% 567.4 – 588.6 nm
  • Center Wavelength of 578 nm and GMBW of 21.2 nm
  • FWHM of 26.1 nm

FL-004616

FF02-438/24-25

FL-004649

Each
  • Tavg > 93% 426 – 450 nm
  • Center Wavelength of 438 nm and GMBW of 24 nm
  • FWHM of 29.5 nm

FL-004649

FF459/526/596-Di01-25x36

FL-007091

Each
  • Dichroic Beamsplitter with edges at 459 nm, 526 nm & 596 nm
  • Ravg > 95% 350 – 450 nm, Ravg > 95% 497.6 – 519.5 nm & Ravg > 95% 567.4 – 588.6 nm
  • Tavg > 93% 464 – 486 nm, Tavg > 93% 532 – 554 nm & Tavg > 93% 603 – 800 nm

FL-007091

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

LED-CFP/YFP/mCherry-3X-A-NQF

S-000302 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

LED-CFP/YFP/mCherry-3X-A-NTE

S-000303 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

LED-CFP/YFP/mCherry-3X-A-OMF

S-001642 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

LED-CFP/YFP/mCherry-3X-A-ZHE

S-000306 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

LED-CFP/YFP/mCherry-3X-A-OFF

S-001640 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

LED-CFP/YFP/mCherry-3X-A-000-ZERO

S-001050 / 0

Out of Stock Backordered In Stock

Standard size -ZERO pixel set

LED-CFP/YFP/mCherry-3X-A-NQF-ZERO

S-001639 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series