Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.
This 7-filter "Sedat" triple-band set is designed for high-speed, sequential imaging of CFP, YFP, and mCherry (and other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter with three single-band emitters intended to be mounted in filter wheels and three single-band exciters which can be installed into the LED light engine or mounted in filter wheels.
Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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3,079.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-482/25-25
FL-004256 / 0
- Tavg > 93% 469.5 – 494.5 nm
- Center Wavelength of 482 nm and GMBW of 25 nm
- FWHM of 30.1 nm
FF01-509/22-25
FL-004258 / 0
- Tavg > 93% 497.6 – 519.5 nm
- Center Wavelength of 508.6 nm and GMBW of 21.9 nm
- FWHM of 26.1 nm
FF02-641/75-25
FL-004436 / 0
- Tavg > 93% 604 – 679 nm
- Center Wavelength of 641.5 nm and GMBW of 75 nm
- FWHM of 81.7 nm
FF01-578/21-25
FL-004616 / 0
- Tavg > 93% 567.4 – 588.6 nm
- Center Wavelength of 578 nm and GMBW of 21.2 nm
- FWHM of 26.1 nm
FF02-438/24-25
FL-004649 / 0
- Tavg > 93% 426 – 450 nm
- Center Wavelength of 438 nm and GMBW of 24 nm
- FWHM of 29.5 nm
FF01-544/24-25
FL-004684 / 0
- Tavg > 93% 532 – 556 nm
- Center Wavelength of 544 nm and GMBW of 24 nm
- FWHM of 29.1 nm
FF459/526/596-Di01-25x36
FL-007091 / 0
- Dichroic Beamsplitter with edges at 459 nm, 526 nm & 596 nm
- Ravg > 95% 350 – 450 nm, Ravg > 95% 426 – 450 nm, Ravg > 95% 497.6 – 519.5 nm & Ravg > 95% 567.4 – 588.6 nm
- Tavg > 93% 464 – 486 nm, Tavg > 93% 532 – 554 nm & Tavg > 93% 603 – 800 nm
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This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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| 6-carboxyrhodamine 6G | 525 | 555 |
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| 6-JOE | 520 | 548 |
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Acridine Yellow | 470 | 550 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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| Alexa Fluor® 514 | 518 | 543 |
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| Alexa Fluor® 532 | 534 | 553 |
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| Alexa Fluor® 568 | 579 | 603 |
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| Alexa Fluor® 594 | 590 | 618 |
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| Alexa Fluor® 660 | 663 | 691 |
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| APC (Allophycocyanin) | 651 | 660 |
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| Aqua 431 | 430 | 478 |
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| AsRed 2 | 576 | 592 |
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| ATTO 425 | 436 | 484 |
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| ATTO 465 | 453 | 507 |
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| ATTO 488 | 500 | 525 |
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| ATTO 495 | 495 | 527 |
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| ATTO 520 | 516 | 538 |