BrightLine® Sedat filter set, optimized for CFP, YFP, & mCherry and other like fluorophores, illuminated with LED-based light engines

Semrock Part Number: LED-CFP/YFP/mCherry-3X3M-A-NTE

IDEX Part Number: S-000299  /

Semrock

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This 7-filter "Sedat" triple-band set is designed for high-speed, sequential imaging of CFP, YFP, and mCherry (and other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter with three single-band emitters intended to be mounted in filter wheels and three single-band exciters which can be installed into the LED light engine or mounted in filter wheels.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

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    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 3,189.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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FF01-482/25-25

FL-004256 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 469.5 – 494.5 nm
  • Center Wavelength of 482 nm and GMBW of 25 nm
  • FWHM of 30.1 nm

FF01-509/22-25

FL-004258 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 497.6 – 519.5 nm
  • Center Wavelength of 508.6 nm and GMBW of 21.9 nm
  • FWHM of 26.1 nm

FF02-641/75-25

FL-004436 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 604 – 679 nm
  • Center Wavelength of 641.5 nm and GMBW of 75 nm
  • FWHM of 81.7 nm

FF01-578/21-25

FL-004616 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 567.4 – 588.6 nm
  • Center Wavelength of 578 nm and GMBW of 21.2 nm
  • FWHM of 26.1 nm

FF02-438/24-25

FL-004649 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 426 – 450 nm
  • Center Wavelength of 438 nm and GMBW of 24 nm
  • FWHM of 29.5 nm

FF01-544/24-25

FL-004684 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 532 – 556 nm
  • Center Wavelength of 544 nm and GMBW of 24 nm
  • FWHM of 29.1 nm

FF459/526/596-Di01-25x36

FL-007091 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edges at 459 nm, 526 nm & 596 nm
  • Ravg > 95% 350 – 450 nm, Ravg > 95% 426 – 450 nm, Ravg > 95% 497.6 – 519.5 nm & Ravg > 95% 567.4 – 588.6 nm
  • Tavg > 93% 464 – 486 nm, Tavg > 93% 532 – 554 nm & Tavg > 93% 603 – 800 nm

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This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept 2 mm dichroic)
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-carboxy-2,7-dichlorofluorescein 500 525
5-carboxynapthofluorescein (pH 10) 555 615
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
6-carboxyrhodamine 6G 525 555
6-JOE 520 548
7-AAD (7-aminoactinomycin D) 548 648
Acridine Orange (+DNA) 502 526
Acridine Yellow 470 550
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 514 518 543
Alexa Fluor® 532 534 553
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
Alexa Fluor® 660 663 691
APC (Allophycocyanin) 651 660
Aqua 431 430 478
AsRed 2 576 592
ATTO 425 436 484
ATTO 465 453 507
ATTO 488 500 525
ATTO 495 495 527
ATTO 520 516 538