Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.
This 7-filter "Sedat" triple-band set is designed for high-speed, sequential imaging of CFP, YFP, and mCherry (and other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter with three single-band emitters intended to be mounted in filter wheels and three single-band exciters which can be installed into the LED light engine or mounted in filter wheels.
Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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3,189.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-482/25-25
FL-004256 / 0
- Tavg > 93% 469.5 – 494.5 nm
- Center Wavelength of 482 nm and GMBW of 25 nm
- FWHM of 30.1 nm
FF01-509/22-25
FL-004258 / 0
- Tavg > 93% 497.6 – 519.5 nm
- Center Wavelength of 508.6 nm and GMBW of 21.9 nm
- FWHM of 26.1 nm
FF02-641/75-25
FL-004436 / 0
- Tavg > 93% 604 – 679 nm
- Center Wavelength of 641.5 nm and GMBW of 75 nm
- FWHM of 81.7 nm
FF01-578/21-25
FL-004616 / 0
- Tavg > 93% 567.4 – 588.6 nm
- Center Wavelength of 578 nm and GMBW of 21.2 nm
- FWHM of 26.1 nm
FF02-438/24-25
FL-004649 / 0
- Tavg > 93% 426 – 450 nm
- Center Wavelength of 438 nm and GMBW of 24 nm
- FWHM of 29.5 nm
FF01-544/24-25
FL-004684 / 0
- Tavg > 93% 532 – 556 nm
- Center Wavelength of 544 nm and GMBW of 24 nm
- FWHM of 29.1 nm
FF459/526/596-Di01-25x36
FL-007091 / 0
- Dichroic Beamsplitter with edges at 459 nm, 526 nm & 596 nm
- Ravg > 95% 350 – 450 nm, Ravg > 95% 497.6 – 519.5 nm & Ravg > 95% 567.4 – 588.6 nm
- Tavg > 93% 464 – 486 nm, Tavg > 93% 532 – 554 nm & Tavg > 93% 603 – 800 nm
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This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
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| 2-dodecylresorufin-lipid | 582 | 595 |
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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| 6-carboxyrhodamine 6G | 525 | 555 |
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| 6-JOE | 520 | 548 |
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Acridine Yellow | 470 | 550 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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| Alexa Fluor® 514 | 518 | 543 |
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| Alexa Fluor® 532 | 534 | 553 |
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| Alexa Fluor® 568 | 579 | 603 |
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| Alexa Fluor® 594 | 590 | 618 |
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| Alexa Fluor® 660 | 663 | 691 |
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| APC (Allophycocyanin) | 651 | 660 |
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| Aqua 431 | 430 | 478 |
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| AsRed 2 | 576 | 592 |
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| ATTO 425 | 436 | 484 |
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| ATTO 465 | 453 | 507 |
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| ATTO 488 | 500 | 525 |
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| ATTO 495 | 495 | 527 |
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| ATTO 520 | 516 | 538 |
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| ATTO 532 | 501 | 545 |
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| ATTO 565 | 563 | 592 |
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| ATTO 590 | 594 | 624 |
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| ATTO 594 | 601 | 624 |
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| ATTO 610 | 614 | 634 |
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| BD Horizon V500 | 410 | 500 |
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| BOBO™-1 | 461 | 484 |
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| BOBO™-3 | 570 | 605 |
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| BODIPY R6G | 528 | 547 |
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| BODIPY TR-X phallacidin | 590 | 621 |
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| BV480 - BD Horizon Brilliant™ Violet 480 | 440 | 479 |
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| Calcein | 494 | 514 |
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| Calcium Crimson | 589 | 609 |
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| Calcium Green-1 | 507 | 529 |
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| Carboxynaphthofluorescein | 599 | 674 |
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| CellTrace BODIPY TR methyl ester | 598 | 625 |
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| CellTracker Red CMTPX | 586 | 614 |
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| Cerulean | 434 | 473 |
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| CFP (ECFP) | 433 | 475 |
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| Cy3.5™ | 578 | 591 |
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| CyQUANT GR-DNA | 502 | 523 |
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| DAF-FM-NO | 495 | 519 |
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| DDAO | 648 | 657 |
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