BrightLine® Sedat filter set, optimized for CFP, YFP, & mCherry and other like fluorophores, illuminated with LED-based light engines

Semrock Part Number: LED-CFP/YFP/mCherry-3X3M-A-000

IDEX Part Number: S-001063  /

Semrock

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This 7-filter "Sedat" triple-band set is designed for high-speed, sequential imaging of CFP, YFP, and mCherry (and other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter with three single-band emitters intended to be mounted in filter wheels and three single-band exciters which can be installed into the LED light engine or mounted in filter wheels.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

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  • Price Not Available.
  • 2,780.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
Set Components

FF01-482/25-25

FL-004256

Each
  • Tavg > 93% 469.5 – 494.5 nm
  • Center Wavelength of 482 nm and GMBW of 25 nm
  • FWHM of 30.1 nm

FL-004256

FF01-509/22-25

FL-004258

Each
  • Tavg > 93% 497.6 – 519.5 nm
  • Center Wavelength of 508.6 nm and GMBW of 21.9 nm
  • FWHM of 26.1 nm

FL-004258

FF01-544/24-25

FL-004684

Each
  • Tavg > 93% 532 – 556 nm
  • Center Wavelength of 544 nm and GMBW of 24 nm
  • FWHM of 29.1 nm

FL-004684

FF01-578/21-25

FL-004616

Each
  • Tavg > 93% 567.4 – 588.6 nm
  • Center Wavelength of 578 nm and GMBW of 21.2 nm
  • FWHM of 26.1 nm

FL-004616

FF02-438/24-25

FL-004649

Each
  • Tavg > 93% 426 – 450 nm
  • Center Wavelength of 438 nm and GMBW of 24 nm
  • FWHM of 29.5 nm

FL-004649

FF02-641/75-25

FL-004436

Each
  • Tavg > 93% 604 – 679 nm
  • Center Wavelength of 641.5 nm and GMBW of 75 nm
  • FWHM of 81.7 nm

FL-004436

FF459/526/596-Di01-25x36

FL-007091

Each
  • Dichroic Beamsplitter with edges at 459 nm, 526 nm & 596 nm
  • Ravg > 95% 350 – 450 nm, Ravg > 95% 426 – 450 nm, Ravg > 95% 497.6 – 519.5 nm & Ravg > 95% 567.4 – 588.6 nm
  • Tavg > 93% 464 – 486 nm, Tavg > 93% 532 – 554 nm & Tavg > 93% 603 – 800 nm

FL-007091

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

LED-CFP/YFP/mCherry-3X3M-A-NQF

S-419505 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

LED-CFP/YFP/mCherry-3X3M-A-NTE

S-000299 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

LED-CFP/YFP/mCherry-3X3M-A-OMF

S-000657 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

LED-CFP/YFP/mCherry-3X3M-A-ZHE

S-000300 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

LED-CFP/YFP/mCherry-3X3M-A-OFF

S-001137 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

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TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-carboxy-2,7-dichlorofluorescein 500 525
5-carboxynapthofluorescein (pH 10) 555 615
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
6-carboxyrhodamine 6G 525 555
6-JOE 520 548
7-AAD (7-aminoactinomycin D) 548 648
Acridine Orange (+DNA) 502 526
Acridine Yellow 470 550
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 514 518 543
Alexa Fluor® 532 534 553
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
Alexa Fluor® 660 663 691
APC (Allophycocyanin) 651 660
Aqua 431 430 478
AsRed 2 576 592
ATTO 425 436 484
ATTO 465 453 507
ATTO 488 500 525
ATTO 495 495 527
ATTO 520 516 538
ATTO 532 501 545
ATTO 565 563 592
ATTO 590 594 624
ATTO 594 601 624
ATTO 610 614 634
BD Horizon V500 410 500
BOBO™-1 461 484
BOBO™-3 570 605
BODIPY R6G 528 547
BODIPY TR-X phallacidin 590 621
BV480 - BD Horizon Brilliant™ Violet 480 440 479
Calcein 494 514
Calcium Crimson 589 609
Calcium Green-1 507 529
Carboxynaphthofluorescein 599 674
CellTrace BODIPY TR methyl ester 598 625
CellTracker Red CMTPX 586 614
Cerulean 434 473
CFP (ECFP) 433 475
Cy3.5™ 578 591
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
DDAO 648 657
Dronpa 502 516
DY-415 418 470
DY-505-Phalloidin 503 530
DY-590 582 599
DY-630 634 656
DyLight 488 493 518
DyLight 594 593 618
ECFP 435 475
Eosin 525 546
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Crimson fluorescent microspheres 620 646
FluoSpheres Orange fluorescent microspheres 540 559
FluoSpheres Red fluorescent microspheres 577 607
FluoSpheres Yellow-Green fluorescent microspheres 503 514
FM 1-43 473 579
FM 4-64 505 725
Gold 525 521 551
Green 496 496 520
Green 500 501 524
HcRed1 588 618
HCS LipidTOX Deep Red neutral lipid stain 635 652
HCS LipidTOX Green phospholipidosis 504 536
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 488 497 526
HiLyte Fluor™ 594 592 616